Immobilized β-lactamase on Fe3O4 magnetic nanoparticles for degradation of β-lactam antibiotics in wastewater
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The emergence of antibiotics residues in pharmaceutical industrial wastewater has been a significant environment problem. However, current methods of treating antibiotic-polluted wastewater are inefficient, of high cost and time-consuming. In this study, highly effective enzymatic Fe3O4 magnetic nanoparticles were developed, which is extremely simple and can degrade antibiotics in a fast manner at a low cost. β-Lactamase, a representative enzyme for β-lactam antibiotic degradation, was covalently immobilized on the surface of magnetic nanoparticles modified with amino groups by a simple cross-linking process. The immobilized β-lactamase displayed a wider pH and temperature range for penicillin G degradation than the free enzyme. Meanwhile, the thermostability and storage stability of the immobilized β-lactamase were improved. Fifty milligrams magnetic nanoparticles immobilized with β-lactamase can thoroughly degrade 100 mL penicillin G (5–50 mg L−1) within 5 min. Even if the β-lactamase immobilized on the nanoparticles was reused 35 times in the 5 mg L−1 penicillin G solution, it still kept more than 95% degradation efficiency. These suggest that magnetic nanoparticles immobilized with β-lactamase have a sufficient capacity for degrading antibiotics in wastewater and will serve as a practical and economical solution to antibiotic pollution in pharmaceutical industrial wastewater treatment.
KeywordsAntibiotic pollution β-Lactamase Magnetic nanoparticles Reusability
This work was supported by the National Natural Science Foundation of China (No. 31371014).
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Conflict of interest
The authors report no conflict of interest.
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