Biosafety assessment of municipal wastewater after treatment by Serratia sp. ISTVKR1

  • A. Gupta
  • I. S. ThakurEmail author
Original Paper


The previously described potent biodegrading bacterial strain Serratia sp. ISTVKR1 isolated from sludge of Vasant Kunj Sewage Treatment Plant (VK STP) was used for the study of detoxification of municipal wastewater contaminants with the help of in vitro bioassays conducted on human liver cancer cell line HepG2. The strain has previously been shown to reduce wastewater contaminant load as revealed by a sevenfold reduction in COD and removal of some organic contaminants in the GC–MS analysis of wastewater post-72-h bacterial treatment. Cytotoxicity, genotoxicity and dioxin-like behavior of real municipal wastewater (collected from inlet of VK STP) was assessed before and after bacterial treatment using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline comet and 7-ethoxyresorufin-O-deethylase (EROD) assays, respectively. The bacterial strain was inoculated into MSM broth (pH = 7.2) containing 50% v/v filter-sterilized influent of VK STP and incubated up to 240 h at 30 °C and 130 rpm. Physicochemical and toxicological analyses were performed for the culture samples. The results of bioassays revealed an increase in cell viability by 40.16% and a reduction in the % EROD activity by 58.4% in the 240-h bacteria-treated sample as compared to the 0-h sample. Genotoxicity as indicated by tail moment (TM) and olive tail moment (OTM) was found to reduce by five- and threefold, respectively, after 240-h bacterial treatment. The results of the study confirmed the potency of the bacterial strain in detoxification of wastewater contaminants.


Wastewater Serratia MTT Comet EROD Detoxification 



This study was financially and technically supported by Jawaharlal Nehru University (JNU), New Delhi, India. The authors are thankful to VK STP and Delhi Jal Board authorities for granting permission for wastewater and sludge collection during the entire duration of this study. We express our gratitude to Dr. J.K. Tripathi, School of Environmental Sciences, JNU, New Delhi, for permitting the ICP-AES analysis. The authors are appreciative of fellow researchers of our lab Dr. Pooja Ghosh and Swati for their help in experimental design, Manish Kumar for assistance in wastewater collection and Madan Kumar and Kristina Medhi for their assistance in manuscript editing. The author (Gupta A) expresses her most sincere gratitude to University Grants Commission (UGC), New Delhi, Government of India, for the Research Fellowship.


  1. Abhishek A, Dwivedi A, Tandan N, Kumar U (2017) Comparative bacterial degradation and detoxification of model and kraft lignin from pulp paper wastewater and its metabolites. Appl Water Sci 7:757–767CrossRefGoogle Scholar
  2. Ados Santos AM, Santos D, Au C, Milatovic D, Aschner M, Batoréu MCC (2008) Antioxidants prevent the cytotoxicity of manganese in RBE4 cells. Brain Res 1236:200–205CrossRefGoogle Scholar
  3. Alimba CG, Dhillon V, Bakare AA, Fenech M (2016) Genotoxicity and cytotoxicity of chromium, copper, manganese and lead, and their mixture in WIL2-NS human B lymphoblastoid cells is enhanced by folate depletion. Mutat Res Genet Toxicol Environ Mutagen 798:35–47CrossRefGoogle Scholar
  4. Andersson E, Rotander A, von Kronhelm T, Berggren A, Ivarsson P, Hollert H, Engwall M (2009) AhR agonist and genotoxicant bioavailability in a PAH-contaminated soil undergoing biological treatment. Environ Sci Pollut Res 16:521–530CrossRefGoogle Scholar
  5. Bacchetta R, Maran B, Marelli M, Santo N, Tremolada P (2016) Role of soluble zinc in ZnO nanoparticle cytotoxicity in Daphnia magna: a morphological approach. Environ Res 148:376–385CrossRefGoogle Scholar
  6. Bae W, Chen W, Mulchandani A, Mehra RK (2000) Enhanced bioaccumulation of heavy metals by bacterial cells displaying synthetic phytochelatins. Biotechnol Bioeng 70(5):518–524CrossRefGoogle Scholar
  7. Bolong N, Ismail AF, Salim MR, Matsuura T (2009) A review of the effects of emerging contaminants in wastewater and options for their removal. Desalination 239(1–3):229–246. doi: 10.1016/j.desal.2008.03.020 (ISSN 0011-9164) CrossRefGoogle Scholar
  8. Chiu JM, Degger N, Leung JY, Po BH, Zheng GJ, Richardson BJ, Lau TC, Wu RS (2016) A novel approach for estimating the removal efficiencies of endocrine disrupting chemicals and heavy metals in wastewater treatment processes. Marine Pollut Bull 112:53–57CrossRefGoogle Scholar
  9. Cristani M, Naccari C, Nostro A, Pizzimenti A, Trombetta D, Pizzimenti F (2012) Possible use of Serratia marcescens in toxic metal biosorption (removal). Environ Sci Pollut Res 19(1):161–168CrossRefGoogle Scholar
  10. Cycoń M, Żmijowska A, Wójcik M, Piotrowska-Seget Z (2013) Biodegradation and bioremediation potential of diazinon-degrading Serratia marcescens to remove other organophosphorus pesticides from soils. J Environ Manage 117:7–16CrossRefGoogle Scholar
  11. Das MT, Budhraja V, Mishra M, Thakur IS (2012) Toxicological evaluation of paper mill sewage sediment treated by indigenous dibenzofuran-degrading Pseudomonas sp. Bioresour Technol 110:71–78CrossRefGoogle Scholar
  12. Díaz E (2010) Bacterial degradation of aromatic pollutants: a paradigm of metabolic versatility. Int Microbiol 7:173–180Google Scholar
  13. Dizer H, Wittekindt E, Fischer B, Hansen PD (2002) The cytotoxic and genotoxic potential of surface water and wastewater effluents as determined by bioluminescence, umu-assays and selected biomarkers. Chemosphere 46(2):225–233CrossRefGoogle Scholar
  14. Edwards SJ, Kjellerup BV (2013) Applications of biofilms in bioremediation and biotransformation of persistent organic pollutants, pharmaceuticals/personal care products, and heavy metals. Appl Microbiol Biotechnol 97:9909–9921CrossRefGoogle Scholar
  15. Erkekoglu P, Kocer-Gumusel B (2014) Genotoxicity of phthalates. Toxicol Mech Methods 24:616–626CrossRefGoogle Scholar
  16. Erkekoglu P, Rachidi W, Yuzugullu OG, Giray B, Favier A, Ozturk M, Hincal F (2010) Evaluation of cytotoxicity and oxidative DNA damaging effects of di(2-ethylhexyl)-phthalate (DEHP) and mono(2-ethylhexyl)-phthalate (MEHP) on MA-10 Leydig cells and protection by selenium. Toxicol Appl Pharmacol 1:52–62CrossRefGoogle Scholar
  17. Fatta-Kassinos D, Kalavrouziotis IK, Koukoulakis PH, Vasquez MI (2011) The risks associated with wastewater reuse and xenobiotics in the agroecological environment. Sci Total Environ 409:3555–3563CrossRefGoogle Scholar
  18. Fernandes D, Zanuy S, Bebianno MJ, Porte C (2008) Chemical and biochemical tools to assess pollution exposure in cultured fish. Environ Pollut 152(1):138–146CrossRefGoogle Scholar
  19. Friha I, Bradai M, Johnson D, Hilal N, Loukil S, Amor FB, Feki F, Han J, Isoda H, Sayadi S (2015) Treatment of textile wastewater by submerged membrane bioreactor: in vitro bioassays for the assessment of stress response elicited by raw and reclaimed wastewater. J Environ Manage 160:184–192CrossRefGoogle Scholar
  20. García-García JD, Sánchez-Thomas R, Moreno-Sánchez R (2016) Bio-recovery of non-essential heavy metals by intra-and extracellular mechanisms in free-living microorganisms. Biotechnol Adv. doi: 10.1016/j.biotechadv.2016.05.003 (ISSN 0734-9750) CrossRefGoogle Scholar
  21. Gautam SK, Sharma D, Tripathi JK, Ahirwar S, Singh SK (2013) A study of the effectiveness of sewage treatment plants in Delhi region. Appl Water Sci 3:57–65CrossRefGoogle Scholar
  22. Ghosh P, Das MT, Thakur IS (2014) Mammalian cell line-based bioassays for toxicological evaluation of landfill leachate treated by Pseudomonas sp. ISTDF1. Environ Sci Pollut Res 21:8084–8094CrossRefGoogle Scholar
  23. Grung M, Lichtenthaler R, Ahel M, Tollefsen KE, Langford K, Thomas KV (2007) Effects-directed analysis of organic toxicants in wastewater effluent from Zagreb, Croatia. Chemosphere 67:108–120CrossRefGoogle Scholar
  24. Gupta A, Thakur IS (2015) Biodegradation of wastewater organic contaminants using Serratia sp. ISTVKR1 isolated from sewage sludge. Biochem Eng J 102:115–124CrossRefGoogle Scholar
  25. Gupta A, Thakur IS (2016) Study of optimization of wastewater contaminant removal along with extracellular polymeric substances (EPS) production by a thermotolerant Bacillus sp. ISTVK1 isolated from heat shocked sewage sludge. Bioresour Technol 213:21–30CrossRefGoogle Scholar
  26. Haq I, Kumar S, Kumari V, Singh SK, Raj A (2016) Evaluation of bioremediation potentiality of ligninolytic Serratia liquefaciens for detoxification of pulp and paper mill effluent. J Hazard Mater 305:190–199CrossRefGoogle Scholar
  27. Harabawy AS, Mosleh YY (2014) The role of vitamins A, C, E and selenium as antioxidants against genotoxicity and cytotoxicity of cadmium, copper, lead and zinc on erythrocytes of Nile tilapia, Oreochromis niloticus. Ecotoxicol Environ Saf 104:28–35CrossRefGoogle Scholar
  28. Harrison EZ, Oakes SR, Hysell M, Hay A (2006) Organic chemicals in sewage sludges. Sci Total Environ 367:481–497CrossRefGoogle Scholar
  29. Houk VS (1992) The genotoxicity of industrial wastes and effluents: a review. Mutat Res Rev Genet Toxicol 277:91–138CrossRefGoogle Scholar
  30. Hrubik J, Glisic B, Tubic A, Ivancev-Tumbas I, Kovacevic R, Samardzija D, Andric N, Kaisarevic S (2016) Toxicological and chemical investigation of untreated municipal wastewater: fraction-and species-specific toxicity. Ecotoxicol Environ Saf 127:153–162CrossRefGoogle Scholar
  31. Humphrey CC, Codi-King S, Klumpp DW (2007) A multibiomarker approach in barramundi (Lates calcarifer) to measure exposure to contaminants in estuaries of tropical North Queensland. Mar Pollut Bull 54:1569–1581CrossRefGoogle Scholar
  32. Isquith A, Matheson D, Slesinski R (1988) Genotoxicity studies on selected organosilicon compounds: in vitro assays. Food Chem Toxicol 26:255–261CrossRefGoogle Scholar
  33. Jadhav JP, Kalyani DC, Telke AA, Phugare SS, Govindwar SP (2010) Evaluation of the efficacy of a bacterial consortium for the removal of color, reduction of heavy metals, and toxicity from textile dye effluent. Bioresour Technol 101:165–173CrossRefGoogle Scholar
  34. Jamwal P, Mittal AK, Mouchel JM (2009) Efficiency evaluation of sewage treatment plants with different technologies in Delhi (India). Environ Monit Assess 153:293–305CrossRefGoogle Scholar
  35. Karve M, Choudhary B (2016) Penicillium chrysogenum immobilised silica: flame atomic absorption spectrometric Pb determination in industrial effluent, sludge and food samples. Int J Environ Sci Technol. doi: 10.1007/s13762-016-1199-5 CrossRefGoogle Scholar
  36. Karvelas M, Katsoyiannis A, Samara C (2003) Occurrence and fate of heavy metals in the wastewater treatment process. Chemosphere 53:1201–1210CrossRefGoogle Scholar
  37. Kesalkar VP, Khedikar IP, Sudame AM (2012) Physico-chemical characteristics of wastewater from paper industry. Int J Eng Res Appl 2:137–143Google Scholar
  38. Kiran MG, Pakshirajan K, Das G (2016) Heavy metal removal from multicomponent system by sulfate reducing bacteria: mechanism and cell surface characterization. J Hazard Mater. doi: 10.1016/j.jhazmat.2015.12.042 (ISSN 0304-3894) CrossRefGoogle Scholar
  39. Kuppusamy S, Thavamani P, Megharaj M, Lee YB, Naidu R (2016) Polyaromatic hydrocarbon (PAH) degradation potential of a new acid tolerant, diazotrophic P-solubilizing and heavy metal resistant bacterium Cupriavidus sp. MTS-7 isolated from long-term mixed contaminated soil. Chemosphere 162:31–39CrossRefGoogle Scholar
  40. Lah B, Gorjanc G, Nekrep FV, Marinsek-Logar R (2004) Comet assay assessment of wastewater genotoxicity using yeast cells. Bull Environ Contam Toxicol 72:607–616CrossRefGoogle Scholar
  41. Laville N, Aït-Aïssa S, Gomez E, Casellas C, Porcher JM (2004) Effects of human pharmaceuticals on cytotoxicity, EROD activity and ROS production in fish hepatocytes. Toxicology 196:41–55CrossRefGoogle Scholar
  42. Lemieux CL, Lynes KD, White PA, Lundstedt S, Oberg L, Lambert IB (2009) Mutagenicity of an aged gasworks soil during bioslurry treatment. Environ Mol Mutagen 50:404–412CrossRefGoogle Scholar
  43. Leung WC, Wong MF, Chua H, Lo W, Yu PHF, Leung CK (2000) Removal and recovery of heavy metals by bacteria isolated from activated sludge treating industrial effluents and municipal wastewater. Water Sci Technol 41:233–240CrossRefGoogle Scholar
  44. Lo N, Kim KH, Baek K, Jia B, Jeon CO (2015) Aestuariicella hydrocarbonica gen. nov., sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from a sea tidal flat. Int J Syst Evol Microbiol 65:1935–1940CrossRefGoogle Scholar
  45. Lü X, Bao X, Huang Y, Qu Y, Lu H, Lu Z (2009) Mechanisms of cytotoxicity of nickel ions based on gene expression profiles. Biomaterials 30:141–148CrossRefGoogle Scholar
  46. Lundstedt S, Haglund P, Oberg L (2003) Degradation and formation of polycyclic aromatic compounds during bioslurry treatment of an acid aged gasworks soil. Environ Toxicol Chem 22:1413–1420CrossRefGoogle Scholar
  47. Ma M, Li J, Wang Z (2005) Assessing the detoxication efficiencies of wastewater treatment processes using a battery of bioassays/biomarkers. Arch Environ Contam Toxicol 49:480–487CrossRefGoogle Scholar
  48. Magaye R, Zhao J (2012) Recent progress in studies of metallic nickel and nickel-based nanoparticles’ genotoxicity and carcinogenicity. Environ Toxicol Pharmacol 34:644–650CrossRefGoogle Scholar
  49. Maier S, Reich E, Martin R, Bachem M, Altug V, Hautmann RE, Gschwend JE (2000) Tributyrin induces differentiation, growth arrest and apoptosis in androgen-sensitive and androgen-resistant human prostate cancer cell lines. Int J Cancer 88:245–251CrossRefGoogle Scholar
  50. Mankidy R, Wiseman S, Ma H, Giesy JP (2013) Biological impact of phthalates. Toxicol Lett 217:50–58CrossRefGoogle Scholar
  51. Miyamae Y, Yamamoto M, Sasaki YF, Kobayashi H, Igarashi-Soga M, Shimoi K, Hayashi M (1998) Evaluation of a tissue homogenization technique that isolates nuclei for the in vivo single cell gel electrophoresis comet assay: a collaborative study by five laboratories. Mutat Res Genet Toxicol Environ Mutagen 418:131–140CrossRefGoogle Scholar
  52. Mosmann T (1983) Rapid calorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63CrossRefGoogle Scholar
  53. Mullen MD, Wolf DC, Ferris FG, Beveridge TJ, Flemming CA, Bailey GW (1989) Bacterial sorption of heavy metals. Appl Environ Microbiol 55:3143–3149Google Scholar
  54. Musa MA, Badisa VL, Latinwo LM, Waryoba C, Ugochukwu N (2010) In vitro cytotoxicity of benzopyranone derivatives with basic side chain against human lung cell lines. Anticancer Res 30:4613–4617Google Scholar
  55. Musa MA, Badisa VL, Latinwo LM, Patterson TA, Owens MA (2012) Coumarin-based benzopyranone derivatives induced apoptosis in human lung (A549) cancer cells. Anticancer Res 32:4271–4276Google Scholar
  56. Nemeikaitė-Čėnienė A, Imbrasaitė A, Sergedienė E, Čėnas N (2005) Quantitative structure–activity relationships in prooxidant cytotoxicity of polyphenols: role of potential of phenoxyl radical/phenol redox couple. Arch Biochem Biophys 441:182–190CrossRefGoogle Scholar
  57. Nikolić MV, Mijajlović MŽ, Jevtić VV, Ratković ZR, Novaković SB, Bogdanović GA, Milovanović J, Arsenijević A, Stojanović B, Trifunović SR, Radić GP (2016) Cytotoxicity of copper (II)-complexes with some S-alkyl derivatives of thiosalicylic acid. Crystal structure of the binuclear copper (II)-complex with S-ethyl derivative of thiosalicylic acid. J Mol Struct 1116:264–271CrossRefGoogle Scholar
  58. Patel TL, Patel BC, Kadam AA, Tipre DR, Dave SR (2015) Application of novel consortium TSR for treatment of industrial dye manufacturing effluent with concurrent removal of ADMI, COD, heavy metals and toxicity. Water Sci Technol 71:1293–1300CrossRefGoogle Scholar
  59. Popa P, Timofti M, Voiculescu M, Dragan S, Trif C, Georgescu LP (2012) Study of physico-chemical characteristics of wastewater in an urban agglomeration in Romania. Sci World J. doi: 10.1100/2012/549028 CrossRefGoogle Scholar
  60. Raj A, Kumar S, Haq I, Singh SK (2014) Bioremediation and toxicity reduction in pulp and paper mill effluent by newly isolated ligninolytic Paenibacillus sp. Ecol Eng 71:355–362CrossRefGoogle Scholar
  61. Ramadass K, Megharaj M, Venkateswarlu K, Naidu R (2016) Soil bacterial strains with heavy metal resistance and high potential in degrading diesel oil and n-alkanes. Int J Environ Sci Technol 13:2863–2874CrossRefGoogle Scholar
  62. Rattier M, Reungoat J, Keller J, Gernjak W (2014) Removal of micropollutants during tertiary wastewater treatment by biofiltration: role of nitrifiers and removal mechanisms. Water Res 54:89–99CrossRefGoogle Scholar
  63. Reifferscheid G, Ziemann C, Fieblinger D, Dill F, Gminski R, Grummt HJ, Hafner C, Hollert H, Kunz S, Rodrigo G, Stopper H (2008) Measurement of genotoxicity in wastewater samples with the in vitro micronucleus test—results of a round-robin study in the context of standardisation according to ISO. Mutat Res Genet Toxicol Environ Mutagen 649:15–27CrossRefGoogle Scholar
  64. Rinaldi DE, Corradi GR, Cuesta LM, Adamo HP, de Tezanos PF (2015) The Parkinson-associated human P 5B-ATPase ATP13A2 protects against the iron-induced cytotoxicity. Biochim Biophys Acta Biomembr 1848:1646–1655CrossRefGoogle Scholar
  65. Roszak J, Catalán J, Järventaus H, Lindberg HK, Suhonen S, Vippola M, Stępnik M, Norppa H (2016) Effect of particle size and dispersion status on cytotoxicity and genotoxicity of zinc oxide in human bronchial epithelial cells. Mutat Res Genet Toxicol Environ Mutagen 805:7–18CrossRefGoogle Scholar
  66. Sanderson H, Johnson DJ, Wilson CJ, Brain RA, Solomon KR (2003) Probabilistic hazard assessment of environmentally occurring pharmaceuticals toxicity to fish, daphnids and algae by ECOSAR screening. Toxicol Lett 144:383–395CrossRefGoogle Scholar
  67. Sarkar T, Butcher RJ, Banerjee S, Mukherjee S, Hussain A (2016) Visible light-induced cytotoxicity of a dinuclear iron (III) complex of curcumin with low-micromolar IC 50 value in cancer cells. Inorg Chim Acta 439:8–17CrossRefGoogle Scholar
  68. Sastry SVAR, Rao BS, Nahata K (2013) Treatment and study of parameters from municipal waste water from urban town. Int J Emerg Technol Adv Eng 3:946–950Google Scholar
  69. Sathya A, Kanaganahalli V, Srinivas Rao P, Gopalakrishnan S (2016) Chapter 12—Cultivation of sweet sorghum on heavy metal-contaminated soils by phytoremediation approach for production of bioethanol. In: Prasad MNV (ed) Bioremediation and bioeconomy. Elsevier, Amsterdam, pp 271–292. doi: 10.1016/B978-0-12-802830-8.00012-5. ISBN 9780128028308CrossRefGoogle Scholar
  70. Schrder CP, Maurer HR (2001) Tributyrin enhances the cytotoxic activity of interleukin-2/interleukin-12 stimulated human natural killer cells against LS 174T colon cancer cells in vitro. Cancer Immunol Immunother 2:69–76CrossRefGoogle Scholar
  71. Singh J, Thakur IS (2015) Evaluation of cyanobacterial endolith Leptolyngbya sp. ISTCY101, for integrated wastewater treatment and biodiesel production: a toxicological perspective. Algal Res 11:294–303CrossRefGoogle Scholar
  72. Singh NP, McCoy MT, Tice R, Schneider EL (1988) A simple technique for quantification of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191CrossRefGoogle Scholar
  73. Singh N, Jenkins GJ, Nelson BC, Marquis BJ, Maffeis TG, Brown AP, Williams PM, Wright CJ, Doak SH (2012) The role of iron redox state in the genotoxicity of ultrafine superparamagnetic iron oxide nanoparticles. Biomaterials 33:163–170CrossRefGoogle Scholar
  74. Smital T, Terzic S, Zaja R, Senta I, Pivcevic B, Popovic M, Mikac I, Tollefsen KE, Thomas KV, Ahel M (2011) Assessment of toxicological profiles of the municipal wastewater effluents using chemical analyses and bioassays. Ecotoxicol Environ Saf 74:844–851CrossRefGoogle Scholar
  75. Song M, Luo C, Li F, Jiang L, Wang Y, Zhang D, Zhang G (2015) Anaerobic degradation of polychlorinated biphenyls (PCBs) and polychlorinated biphenyls ethers (PBDEs), and microbial community dynamics of electronic waste-contaminated soil. Sci Total Environ 502:426–433CrossRefGoogle Scholar
  76. USEPA (1982) Determination of metals and trace elements in water and wastes by inductively coupled plasma-atomic emission spectrometry, Revision 4.4 EMMC Version. Office of Research and Development. Accessed June 2013
  77. Valls M, De Lorenzo V (2002) Exploiting the genetic and biochemical capacities of bacteria for the remediation of heavy metal pollution. FEMS Microbiol Rev 26:327–338CrossRefGoogle Scholar
  78. Vanhulle S, Trovaslet M, Enaud E, Lucas M, Taghavi S, Van Der Lelie D, Van Aken B, Foret M, Onderwater RC, Wesenberg D, Agathos SN (2007) Decolorization, cytotoxicity, and genotoxicity reduction during a combined ozonation/fungal treatment of dye-contaminated wastewater. Environ Sci Technol 42:584–589CrossRefGoogle Scholar
  79. Wang YF, Shyu HW, Chang YC, Tseng WC, Huang YL, Lin KH, Chou MC, Liu HL, Chen CY (2012) Nickel (II)-induced cytotoxicity and apoptosis in human proximal tubule cells through a ROS-and mitochondria-mediated pathway. Toxicol Appl Pharmacol 259:177–186CrossRefGoogle Scholar
  80. Wang S, Fang H, Yi X, Xu Z, Xie X, Tang Q, Ou M, Xu X (2016) Oxidative removal of phenol by HRP-immobilized beads and its environmental toxicology assessment. Ecotoxicol Environ Saf 130:234–239CrossRefGoogle Scholar
  81. Yang S, Hai FI, Price WE, McDonald J, Khan SJ, Nghiem LD (2016) Occurrence of trace organic contaminants in wastewater sludge and their removals by anaerobic digestion. Bioresour Technol 210:153–159CrossRefGoogle Scholar
  82. Žegura B, Heath E, Černoša A, Filipič M (2009) Combination of in vitro bioassays for the determination of cytotoxic and genotoxic potential of wastewater, surface water and drinking water samples. Chemosphere 75:1453–1460CrossRefGoogle Scholar
  83. Zhang J, Falany JL, Xie X, Falany CN (2000) Induction of rat hepatic drug metabolizing enzymes by dimethylcyclosiloxanes. Chem Biol Interact 124:133–147CrossRefGoogle Scholar
  84. Zhang C, Jia L, Wang S, Qu J, Li K, Xu L, Shi Y, Yan Y (2010) Biodegradation of beta-cypermethrin by two Serratia spp. with different cell surface hydrophobicity. Appl Microbiol Biotechnol 101:3423–3429Google Scholar
  85. Zhang J, Zhang Y, Liu W, Quan X, Chen S, Zhao H, Jin Y, Zhang W (2013) Evaluation of removal efficiency for acute toxicity and genotoxicity on zebrafish in anoxic–oxic process from selected municipal wastewater treatment plants. Chemosphere 90:2662–2666CrossRefGoogle Scholar

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© Islamic Azad University (IAU) 2017

Authors and Affiliations

  1. 1.School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiIndia

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