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Biosafety assessment of municipal wastewater after treatment by Serratia sp. ISTVKR1

  • A. Gupta
  • I. S. Thakur
Original Paper

Abstract

The previously described potent biodegrading bacterial strain Serratia sp. ISTVKR1 isolated from sludge of Vasant Kunj Sewage Treatment Plant (VK STP) was used for the study of detoxification of municipal wastewater contaminants with the help of in vitro bioassays conducted on human liver cancer cell line HepG2. The strain has previously been shown to reduce wastewater contaminant load as revealed by a sevenfold reduction in COD and removal of some organic contaminants in the GC–MS analysis of wastewater post-72-h bacterial treatment. Cytotoxicity, genotoxicity and dioxin-like behavior of real municipal wastewater (collected from inlet of VK STP) was assessed before and after bacterial treatment using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), alkaline comet and 7-ethoxyresorufin-O-deethylase (EROD) assays, respectively. The bacterial strain was inoculated into MSM broth (pH = 7.2) containing 50% v/v filter-sterilized influent of VK STP and incubated up to 240 h at 30 °C and 130 rpm. Physicochemical and toxicological analyses were performed for the culture samples. The results of bioassays revealed an increase in cell viability by 40.16% and a reduction in the % EROD activity by 58.4% in the 240-h bacteria-treated sample as compared to the 0-h sample. Genotoxicity as indicated by tail moment (TM) and olive tail moment (OTM) was found to reduce by five- and threefold, respectively, after 240-h bacterial treatment. The results of the study confirmed the potency of the bacterial strain in detoxification of wastewater contaminants.

Keywords

Wastewater Serratia MTT Comet EROD Detoxification 

Notes

Acknowledgements

This study was financially and technically supported by Jawaharlal Nehru University (JNU), New Delhi, India. The authors are thankful to VK STP and Delhi Jal Board authorities for granting permission for wastewater and sludge collection during the entire duration of this study. We express our gratitude to Dr. J.K. Tripathi, School of Environmental Sciences, JNU, New Delhi, for permitting the ICP-AES analysis. The authors are appreciative of fellow researchers of our lab Dr. Pooja Ghosh and Swati for their help in experimental design, Manish Kumar for assistance in wastewater collection and Madan Kumar and Kristina Medhi for their assistance in manuscript editing. The author (Gupta A) expresses her most sincere gratitude to University Grants Commission (UGC), New Delhi, Government of India, for the Research Fellowship.

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© Islamic Azad University (IAU) 2017

Authors and Affiliations

  1. 1.School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiIndia

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