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Neotropical Entomology

, Volume 48, Issue 4, pp 594–603 | Cite as

Genetic Analysis of Populations of the Peach Fruit Fly, Bactrocera zonata (Diptera: Tephritidae), in Iran

  • M. Koohkanzadeh
  • P. Pramual
  • L. FekratEmail author
Systematics, Morphology and Physiology
  • 116 Downloads

Abstract

Bactrocera zonata (Saunders) as one of the most devastating species of the genus Bactrocera is a notorious polyphagous fruit fly pest attacking lots of commercially important host plant species in many parts of the world including Iran. Despite economic importance, the population genetics of B. zonata have remained relatively unexplored in the country. In this study, the genetic structure, genetic diversity, and demographic history of this pest in Iran were investigated on the basis of cytochrome oxidase c subunit 1 (COI) sequences. A total of 21 haplotypes were identified from 66 individuals collected from seven locations. Genetic diversity indices (number of haplotypes, haplotype diversity, and nucleotide diversity) revealed high genetic diversity without any isolation by distance among the geographic areas. An overall low level of genetic differentiation between populations revealed by genetic structure analysis reinforces the hypothesis of free movement of flies throughout the geographic areas. Non-significant correlation between genetic distances and geographic distances was an indication of a high level of gene flow among the studied populations of the pest. In addition, the recent population expansion following a recent past bottleneck could be a factor that might be contributed to the overall low level of the genetic structure. Mismatch distribution analysis as well as Tajima’s D and Fu’s FS tests strengthens the likelihood of a recent expanded population following a population bottleneck.

Keywords

Bactrocera zonata genetic diversity genetic structure peach fruit fly Tephritidae mitochondrial DNA 

Notes

Author Contribution Statement

LF and MK planned, designed, and executed experimental work; LF and PP conducted data analyses; LF wrote the manuscript; and PP edited the manuscript.

Funding Information

This study received financial support from the Research Deputy of Ferdowsi University of Mashhad.

Supplementary material

13744_2018_659_MOESM1_ESM.pdf (922 kb)
Supplementary file 1 (PDF 921 kb)

References

  1. Agarwal ML, Kumar P, Kumar V (1999) Population suppression of Bactrocera dorsalis (Hendel) by Bactrocera zonata (Saunders) (Diptera: Tephritidae) in North Bihar. Shashpa 6:189–191Google Scholar
  2. Aketarawong N, Isasawin S, Sojikul P, Thanaphum S (2015) Gene flow and genetic structure of Bactrocera carambolae (Diptera, Tephritidae) among geographical differences and sister species, B. dorsalis, inferred from microsatellite DNA data. Zookeys 540:239–272CrossRefGoogle Scholar
  3. Alberti AC, Rodriguero MS, Cendra PG, Saidman BO, Vilardi JC (2002) Evidence indicating that Argentine populations of Anastrepha fraterculus (Diptera: Tephritidae) belong to a single biological species. Ann Entomol Soc Am 92:505–512CrossRefGoogle Scholar
  4. Amin AA (2003) Studies on the peach fruit fly, Bactrocera zonata (Saunders) and its control in Fayoum Governorate [Msc thesis]. Fayoum: Faculty of Agriculture, Cairo University, p. 127Google Scholar
  5. Asokan R, Rebijith KB, Singh SK, Sidhu AS, Siddharthan S, Karanth PK, Ellango R, Ramamurthy VV (2011) Molecular identification and phylogeny of Bactrocera species (Diptera: Tephritidae). Fla Entomol 94(4):1026–1035Google Scholar
  6. Bagheri A, Kolyaee R, Askari Seyahooei M, Modaress Najafabadi SS, Faraji G (2017) Efficacy of methyl eugenol bait traps for controlling the mango fruit fly Bactrocera zonata (Diptera: Tephritidae). J Crop Prot 6(2):181–189Google Scholar
  7. Bandelt H, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16(1):37–48CrossRefGoogle Scholar
  8. Blacket MJ, Semeraro L, Malipatil MB (2012) Barcoding Queensland fruit flies (Bactrocera tryoni): impediments and improvements. Mol Ecol Resour 12:428–436CrossRefGoogle Scholar
  9. Bohonak AJ (2002) IBD (Isolation by Distance): a programme for analysis of isolation by distance. Heredity 93:153–154CrossRefGoogle Scholar
  10. Boykin LM, Schutze MK, Krosch MN, Chomič A, Chapman TA, Englezou A, Armstrong KF, Clarke AR, Hailstones D, Cameron SL (2014) Multi-gene phylogenetic analysis of Southeast Asian pest members of the Bactrocera dorsalis species complex (Diptera: Tephritidae) does not support current taxonomy. J Appl Entomol 138:235–253CrossRefGoogle Scholar
  11. Butani DK (1976) Insect pests of fruit crops and their control: custard apple. Pesticides 10:27–28Google Scholar
  12. Butani DK, Verma S (1977) Pests of vegetables and their control: cucurbits. Pesticides 11:37–41Google Scholar
  13. Choudhary JS, Naaz N, Moarano PCS (2016) Genetic analysis of oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae) populations based on mitochondrial Cox and nad1 gene sequences from India and other Asian countries. Genetica 144:611–623CrossRefGoogle Scholar
  14. Choudhary JS, Naaz N, Lemtur M, Das B, Singh AK, Bhatt BP, Prabhakar CS (2017) Genetic analysis of Bactrocera zonata (Diptera: Tephritidae) populations from India based on cox1 and nad1 gene sequences. Mitochondrial DNA A 29:727–736.  https://doi.org/10.1080/24701394.2017.1350952 CrossRefGoogle Scholar
  15. Delrio G, Cocco A (2012) In: Vacante V, Gerson U (eds) Tephritidae. Integrated control of citrus pests in the Mediterranean region. Bentham Science Publishers, Emirate of Sharjah, pp 206–222Google Scholar
  16. Dogac E, kande Mir I, Taskin V (2013) The genetic polymorphism and colonization process of olive fly populations in Turkey. PLoS One 8:e56067CrossRefGoogle Scholar
  17. Draz KA (2016) Population activity of peach fruit fly Bactrocera zonata (Saunders) (Diptera: Tephritidae) at fruits orchards in Kafer El-Shikh Governorate, Egypt. Arthropods 5(1):58Google Scholar
  18. Duyck PF, Sterlin JF, Quilici S (2004) Survival and development of different life stages of Bactrocera zonata reared at five constant temperatures compared to other fruit fly species. Bull Entomol Res 94:89–93CrossRefGoogle Scholar
  19. Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567CrossRefGoogle Scholar
  20. Fletcher BS (1987) The biology of Dacine fruit flies. Annu Rev Entomol 32:115–144CrossRefGoogle Scholar
  21. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3:294–299Google Scholar
  22. Fu YX (1997) Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147:915–925Google Scholar
  23. Galiano D, Bernardo-Silva J, de Freitas TRO (2014) Genetic pool information reflects highly suitable areas: the case of two parapatric endangered species of tuco-tucos (Rodentia: Ctenomiydae). PLoS One 9(5):e97301CrossRefGoogle Scholar
  24. Ghanim NM (2009) Studies on the peach fruit fly, Bactrocera zonata (Saunders) (Tephritidae, Diptera). PhD Thesis, Fac Agric Mansoura University, EgyptGoogle Scholar
  25. Gilchrist AS, Dominiak B, Gillespie PS, Sved JA (2006) Variation in population structure across the ecological range of the Queensland fruit fly, Bactrocera tryoni. Aust J Zool 54:87–95CrossRefGoogle Scholar
  26. Grewal JS, Malhi CS (1987) Prunus persica Batsch. Damage by birds and fruit fly pests in Ludhiana (Punjab). J Entomol Res 11(1):119–120Google Scholar
  27. Harpending HC (1994) Signature of ancient population growth in a low-resolution mitochondrial DNA mismatch distribution. Hum Biol 66:591–600Google Scholar
  28. Hashem AG, Mohamed SMA, El-Wakkad MF (2001) Diversity and abundance of Mediterranean and peach fruit flies (Diptera: Tephritidae) in different horticultural orchards. Egypt J Appl Sci 16:303–314Google Scholar
  29. Hazkani-Covo E, Zeller RM, Martin W (2010) Molecular poltergeists: mitochondrial DNA copies (numts) in sequenced nuclear genomes. PLoS Genet 6:e1000834CrossRefGoogle Scholar
  30. Hu J, Zhang JL, Nardi F, Zhang RJ (2008) Population genetic structure of the melon fly, Bactrocera cucurbitae (Diptera: Tephritidae), from China and Southeast Asia. Genetica 134:319–324CrossRefGoogle Scholar
  31. Iwahashi O, Routhier W (2001) Aedeagal length and its variation of the peach fruit fly, Bactrocera zonata, which recently invaded Egypt. Appl Entomol Zool 36:13–17CrossRefGoogle Scholar
  32. Jamnongluk W, Baimai V, Kittayapong P (2003) Molecular phylogeny of tephritid fruit flies in the Bactrocera tau complex using the mitochondrial COI sequences. Genome 46:112–118CrossRefGoogle Scholar
  33. Jiang F, Li ZH, Deng YL, Wu JJ, Liu RS, Buahom N (2013) Rapid diagnosis of the economically important fruit fly, Bactrocera correcta (Diptera: Tephritidae) based on a species-specific barcoding cytochrome oxidase I marker. Bull Entomol Resour 103(3):363–371CrossRefGoogle Scholar
  34. Jiang F, Jin Q, Liang L, Zhang AB, Li ZH (2014) Existence of species complex largely reduced barcoding success for invasive species of Tephritidae: a case study in Bactrocera spp. Mol Ecol Resour 14(6):1114–1128CrossRefGoogle Scholar
  35. Kapoor VC, Agarwal ML (1983) Fruit flies and their increasing host plants in India. pp. 252–257 in Proceedings of the CEC/IOBC International Symposium. 16–19 November 1982, Athens, GreeceGoogle Scholar
  36. Khamis FM, Masiga DK, Mohamed SA, Salifu D, de Meyer M, Ekesi S (2012) Taxonomic identity of the invasive fruit fly pest, Bactrocera invadens: concordance in morphometry and DNA barcoding. PLoS One 7(9):E44862CrossRefGoogle Scholar
  37. Kunprom C, Pramual P (2017) Genetic structure and demographic history of the melon fly Zeugodacus cucurbitae (coquillet) (Diptera; Tephritidae) in Thailand. Agric Forest Entomol.  https://doi.org/10.1111/afe.12242
  38. Kunprom C, Sopaladawan PN, Pramual P (2015) Population genetics and demographic history of guava fruit fly Bactrocera correcta (Diptera: Tephritidae) in northeastern Thailand. Eur J Entomol 112(2):227–234CrossRefGoogle Scholar
  39. Liao PC, Kuo DC, Lin CC, Ho KC, Lin TP, Hwang SY (2010) Historical spatial range expansion and a very recent bottleneck of Cinnamomum kanehirae Hay. (Lauraceae) in Taiwan inferred from nuclear genes. BMC Evol Biol 10:124CrossRefGoogle Scholar
  40. Librado P, Rozas J (2009) DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25:1451–1452CrossRefGoogle Scholar
  41. Malacrida AR, Gomulski LM, Bonizzoni M, Bertin S, Gasperi G, Guglielmino CR (2007) Globalization and fruitfly invasion and expansion: the medfly paradigm. Genetica 131:1–9CrossRefGoogle Scholar
  42. Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220Google Scholar
  43. Meeyen K, Nanorksopaldawan P, Pramual P (2014) Population structure, population history and DNA barcoding of fruit fly Bactrocera latifrons (Hendel) (Diptera: Tephritidae). Entomol Sci 17:219–230CrossRefGoogle Scholar
  44. Mun J, Bohonak AJ, Roderick GK (2003) Population structure of the pumpkin fruit fly Bactrocera depressa (Tephritidae) in Korea and Japan: Pliocene allopatry or recent invasion. Mol Ecol 12:2941–2951CrossRefGoogle Scholar
  45. Nardi F, Carapelli A, Dallai R, Roderick GK, Frati F (2005) Population structure and colonization history of the olive fly, Bactrocera oleae (Diptera: Tephritidae). Mol Ecol 14:2729–2738CrossRefGoogle Scholar
  46. Ni WL, Li ZH, Chen HJ, Wan FH, Qu WW, Zhang Z, Kriticos DJ (2012) Including climate change in pest risk assessment: the peach fruit fly, Bactrocera zonata (Diptera: Tephritidae). Bull Entomol Res 102:173–183CrossRefGoogle Scholar
  47. O’Neill SL, Giordano R, Colbert AME, Karr TL, Robertson HM (1992) 16S rRNA phylogenetic analysis of the bacterial endosymbionts associated with cytoplasmic incompatibility in insects. Proc Natl Acad Sci USA 89:2699–2702CrossRefGoogle Scholar
  48. EPPO (European and Mediterranean Plant Protection Organization) (2005) Bulletin OEPP/EPPO 35: 371–373Google Scholar
  49. Peterson MA, Denno RF (1998) The influence of dispersal and diet breadth on patterns of genetic isolation by distance in phytophagous insects. Amer Nat 152:428–446CrossRefGoogle Scholar
  50. Prabhakar CS, Mehta PK, Sood P, Singh SK, Sharma P, Sharma PN (2012a) Population genetic structure of the melon fly, Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae) based on mitochondrial cytochrome oxidase (COI) gene sequences. Genetica 140:83–91CrossRefGoogle Scholar
  51. Prabhakar CS, Sood P, Mehta PK (2012b) Pictorial keys for predominant Bactrocera and Dacus fruit flies (Diptera: Tephritidae) of north western Himalaya. Arthropods 1:101–111Google Scholar
  52. Prabhakar CS, Sood P, Metha PK, Sharma PN (2013) Population genetic structure of the pumpkin fruit fly, Bactrocera tau (Walker) (Diptera: Tephritidae) in Himachal Pradesh, India. Biochem Syst Ecol 51:291–296CrossRefGoogle Scholar
  53. Qin YJ, Buahom N, Krosch MN, Du Y, Wu Y, Malacrida AR, Deng YL, Liu JQ, Jiang XL, Li ZH (2016) Genetic diversity and population structure in Bactrocera correcta (Diptera: Tephritidae) inferred from mtDNA cox1 and microsatellite markers. Sci Rep 6:38476.  https://doi.org/10.1038/srep38476
  54. Roderick GK (1996) Geographic structure of insect populations: gene flow, phylogeography, and their uses. Annu Rev Entomol 41:263–290CrossRefGoogle Scholar
  55. Roderick GK, Navajas M (2003) Genes in new environment: genetics and evolution in biological control. Nat Rev Genet 4:889–899CrossRefGoogle Scholar
  56. Roger AR, Harpending H (1992) Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol 9:552–569Google Scholar
  57. Rosetti N, Remis MI (2012) Spatial genetic structure and mitochondrial DNA phylogeography of Argentinean populations of the grasshopper Dichroplus elongatus. Plos One 7(7):e40807Google Scholar
  58. Shehata NF, Younes MWF, Mahmoud YA (2008) Biological studies on the peach fruit fly, Bactrocera zonata (Saunders) in Egypt. J Appl Sci Res 4:1103–1106Google Scholar
  59. Shi MM, Michalski SG, Welk E, Chen XY, Durka W (2014) Phylogeography of a widespread Asian subtropical tree: genetic east–west differentiation and climate envelope modelling suggest multiple glacial refugia. J Biogeogr 41(9):1710–1720Google Scholar
  60. Shi W, Kerdelhué C, Ye H (2005) Population genetics of the oriental fruit fly, Bactrocera dorsalis (Diptera: Tephritidae), in Yunnan (China) based on mitochondrial DNA sequences. Environ Entomol 34:977–983CrossRefGoogle Scholar
  61. Shi W, Kerdelhué C, Ye H (2010) Population genetic structure of the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae) from Yunnan province (China) and nearby sites across the border. Genetica 138:377–385CrossRefGoogle Scholar
  62. Shi W, Kerdelhué C, Ye H (2012) Genetic structure and inferences on potential source areas for Bactrocera dorsalis (Hendel) based on mitochondrial and microsatellite markers. PLoS One 7:e37083CrossRefGoogle Scholar
  63. Slatkin M, Hudson RR (1991) Pairwise comparisons of mitochondrial DNA sequences in stable and esxponentially growing populations. Genetics 129(2):555–562Google Scholar
  64. Syed RA, Ghani MA, Murtaza M (1970) Studies on the trypetids and their natural enemies in West Pakistan, further observations on Dacus dorsalis, Hendel. Common Wealth Institute of Biological Control Technical Bulletin 13:17–30Google Scholar
  65. Tajima H (1989) Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics 123:585–595Google Scholar
  66. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefGoogle Scholar
  67. Virgilio M, Jordaens K, Verwimp C, White IM, De Meyer M (2015) Higher phylogeny of frugivorous flies (Diptera, Tephritidae, Dacini): localised partition conflicts and a novel generic classification. Mol Phylogenet Evol 85:171–179CrossRefGoogle Scholar
  68. White IM, Elson-Harris MM (1992) Fruit flies of economic significance: their identification and bionomics. CAB International, Wallingford, UKGoogle Scholar
  69. Wu MC, Lee S, Cai T, Li Y, Boehnke M, Lin X (2011) Rare-variant association testing for sequencing data with the sequence kernel association test. Am J Hum Genet 89(1):82–93Google Scholar

Copyright information

© Sociedade Entomológica do Brasil 2019

Authors and Affiliations

  1. 1.Dept of Plant Protection, Faculty of AgricultureFerdowsi University of MashhadMashhadIran
  2. 2.Dept of Biology, Faculty of ScienceMahasarakham UniversityKantharawichai DistrictThailand
  3. 3.Dept of Plant Protection, Faculty of AgricultureFerdowsi University of MashhadMashhadIran

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