Response Mechanism of Oviposition and Relevant Protein Expression of Bactrocera cucurbitae (Coquillet) to Short-Term High-Temperature Conditions
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Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae) is an important pest of vegetables in Asia, the Middle East, Africa, and Hawaii. High temperature can significantly influence B. cucurbitae reproduction. The effect of short-term high-temperature exposure on proteins that affect oviposition was analyzed by proteomics. Among six key target genes for oviposition, the expression of Vitellogenin-1, Vitellogenin-2, and Vitellogenin receptor was similar in B. cucurbitae exposed to higher temperature compared to controls. However, levels of Vitellogenin-3 were reduced. Juvenile hormone (Jh)-inducible protein was downregulated and then upregulated, while the expression of Jh-epoxide hydrolase-2 showed the opposite Jh-inducible protein trend. Therefore, short-term high-temperature stress can cause differential expression of proteins related to oviposition in B. cucurbitae, which in turn further triggers the hormesis of oviposition. High-temperature conditions have become more frequent because of climate warming and are predicted to continue. The data indicate that climate effects on insect reproduction pose a significant threat to agriculture in a world of increasing population.
KeywordsBactrocera cucurbitae (Coquillett) juvenile hormone vitellogenin high temperature iTRAQ climate change
We thank Si-hua Peng, Yan-zuo Chen, and Zi-wen Feng for helping us with insect rearing and the analysis of reproductive biology experiments.
Shihao Zhou participated in the study design and analysis and drafted the manuscript. Shihao Zhou, Bei Zeng, and Wenjing Zhu participated carried out the study and helped to draft the manuscript. Yueguan Fu participated in study design and helped to draft the manuscript. All authors read and approved the final manuscript.
This study was supported by the Natural Science Foundation of Hainan Province of China (317043), Natural Science Foundation of China (31760518), National Key Technology Research and Development Program of the Ministry of Science and Technology of China (2015BAD08B03), the Research Start-up Foundation of Hainan University (kyqd1655), and Hainan University-Tianjin University Independent Innovation Fund Cooperation Project (HDTDU201705).
Compliance with Ethical Standards
The authors declare that they have no competing interests.
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