Synthesis of polyaniline-coated titanium oxide nanoparticles for preconcentration of cobalt (II) followed by electrothermal atomic absorption spectrometry

  • Mohammad Nasser Sadkhan
  • Mohammad EftekhariEmail author
  • Mohammad Gheibi
  • Mahsa Keramati Yazdi
  • Nima Emrani
Original Paper


An efficient and novel adsorbent, polyaniline (PANI)-coated titanium oxide nanoparticle (TiO2-NPs) was applied for preconcentration of ultra-trace levels of Co2+ followed by electrothermal atomic absorption spectrometry. In this method, TiO2-PANI nanoparticles were synthesized by chemical method and used as an adsorbent in solid phase extraction. The scanning electron microscopy, transmission electron microscopy and Fourier transform infrared spectrophotometry were applied to characterize of the synthesized TiO2-PANI nanoparticles. The parameters affecting the extraction recovery including amounts of adsorbent (g), sample solution pH, and extraction and desorption time (min) were optimized by the response surface methodology based on central composite design. The optimum conditions obtained at the pH of 9.0, 10.0 mg amounts of TiO2-PANI nanoparticles, 10.0 min extraction time and 3.50 min desorption time. Also, the linear range of the calibration curve was obtained at 0.10–2.0 µg L−1 Co2+ with a correlation coefficient of 0.9976. The relative standard deviation (RSD, %) was 3.7% for the six times replicate analysis of 0.50 µg L−1 of Co2+ and the limit of detection (LOD) was 0.036 µg L−1 (n = 8). The method’s accuracy was checked by analyzing of the certified reference material and finally, the proposed method was used for the preconcentration and determination of Co2+ in different food and water samples.


Cobalt Polyaniline-coated titanium oxide nanoparticles Solid phase extraction Electrothermal atomic absorption spectrometry Response surface methodology based on central composite design 



All sources of funding for this research including the data collection, analysis and interpretation as well as manuscript writing were supported by all authors. The authors wish to thank Ferdowsi University of Mashhad and University of Neyshabur for their financial support.

Compliance with ethical standards

Conflict of interest

The authors declared that there is not any conflict of interest.


  1. 1.
    G.G. Amatucci, J.M. Tarascon, L.C. Klein, Solid State Ion. 83(1–2), 167 (1996)CrossRefGoogle Scholar
  2. 2.
    A. Marti, Injury 31, D18–D21 (2000)CrossRefGoogle Scholar
  3. 3.
    A.L. Fernández, L. de Pablo, Pigment Resin Technol. 31(6), 350 (2002)CrossRefGoogle Scholar
  4. 4.
    J.C. Ingersoll, N. Mani, J.C. Thenmozhiyal, A. Muthaiah, J. Power Sources 173(1), 450 (2007)CrossRefGoogle Scholar
  5. 5.
    D.A. Rodionov, A.G. Vitreschak, A.A. Mironov, M.S. Gelfand, J. Biol. Chem. 278(42), 41148 (2003)CrossRefGoogle Scholar
  6. 6.
    D. Lison, Crit. Rev. Toxicol. 26(6), 585 (1996)CrossRefGoogle Scholar
  7. 7.
    D. Citak, M. Tuzen, Food Chem. Toxicol. 48(5), 1399 (2010)CrossRefGoogle Scholar
  8. 8.
    A. Safavi, H. Abdollahi, M.R. Hormozi-Nezhad, R. Kamali, Spectrochim. Acta A Mol. Biomol. Spectrosc. 60(12), 2897 (2004)CrossRefGoogle Scholar
  9. 9.
    M. Eftekhari, F. Javedani-Asleh, M. Chamsaz, Food Anal. Methods 9(7), 1985 (2016)CrossRefGoogle Scholar
  10. 10.
    M. Gharehbaghi, F. Shemirani, M. Baghdadi, Int. J. Environ. Anal. Chem. 88(7), 513 (2008)CrossRefGoogle Scholar
  11. 11.
    M. Chamsaz, M. Eftekhari, A. Eftekhari, A. Yekkebashi, Environ. Monit. Assess. 185(11), 9067 (2013)CrossRefGoogle Scholar
  12. 12.
    M. Ghaedi, F. Ahmadi, M. Soylak, J. Hazard. Mater. 147(1–2), 226 (2007)CrossRefGoogle Scholar
  13. 13.
    A.H. Almertaha, M. Eftekhari, M. Chamsaz, M. Gheibi, Environ. Technol. 40(14), 1831 (2019)CrossRefGoogle Scholar
  14. 14.
    M. Eftekhari, M. Gheibi, M. Akrami, F. Iranzad, New J. Chem. 42(2), 1159 (2018)CrossRefGoogle Scholar
  15. 15.
    M. Khodarahmi, M. Eftekhari, M. Gheibi, M. Chamsaz, J. Food Meas. Charact. 12(3), 1485 (2018)CrossRefGoogle Scholar
  16. 16.
    H. Shirkhanloo, A. Khaligh, H.Z. Mousavi, A. Rashidi, Int. J. Environ. Anal. Chem. 95(1), 16 (2015)CrossRefGoogle Scholar
  17. 17.
    C. Duran, A. Gundogdu, V.N. Bulut, M. Soylak, L. Elci, H.B. Sentürk, M. Tüfekci, J. Hazard. Mater. 146(1–2), 347 (2007)CrossRefGoogle Scholar
  18. 18.
    R.S. Praveen, S. Daniel, T.P. Rao, Talanta 66(2), 513 (2005)CrossRefGoogle Scholar
  19. 19.
    M. Faraji, Y. Yamini, A. Saleh, M. Rezaee, M. Ghambarian, R. Hassani, Anal. Chim. Acta 659(1–2), 172 (2010)CrossRefGoogle Scholar
  20. 20.
    S.Z. Mohammadi, H. Hamidian, L. Karimzadeh, Z. Moeinadini, J. Anal. Chem. 68(11), 953 (2013)CrossRefGoogle Scholar
  21. 21.
    A.A. Asgharinezhad, H. Ebrahimzadeh, F. Mirbabaei, N. Mollazadeh, N. Shekari, Anal. Chim. Acta 844, 80 (2014)CrossRefGoogle Scholar
  22. 22.
    M. Rezvani, A.A. Asgharinezhad, H. Ebrahimzadeh, N. Shekari, Microchim. Acta 181, 1887 (2014)CrossRefGoogle Scholar
  23. 23.
    A. Eisazadeh, H. Eisazadeh, K.A. Kassim, Synth. Met. 171, 56 (2013)CrossRefGoogle Scholar
  24. 24.
    H.J. Majeed, M. Eftekhari, M. Gheibi, M. Chamsaz, J. Food Meas. Charact. 13(1), 339 (2019)CrossRefGoogle Scholar
  25. 25.
    S.R. Yousefi, S.J. Ahmadi, Microchim. Acta 172(1–2), 75 (2011)CrossRefGoogle Scholar
  26. 26.
    P. Berton, R.G. Wuilloud, Anal. Methods 3(3), 664 (2011)CrossRefGoogle Scholar
  27. 27.
    Ö. Yalçınkaya, O.M. Kalfa, A.R. Türker, J. Hazard. Mater. 195, 332 (2011)CrossRefGoogle Scholar

Copyright information

© Iranian Chemical Society 2019

Authors and Affiliations

  • Mohammad Nasser Sadkhan
    • 1
  • Mohammad Eftekhari
    • 2
    Email author
  • Mohammad Gheibi
    • 3
  • Mahsa Keramati Yazdi
    • 4
  • Nima Emrani
    • 3
  1. 1.Department of Chemistry, Faculty of SciencesFerdowsi University of MashhadMashhadIran
  2. 2.Department of Chemistry, Faculty of SciencesUniversity of NeyshaburNeyshaburIran
  3. 3.Department of Civil EngineeringFerdowsi University of MashhadMashhadIran
  4. 4.Department of Food Science and Technology, Quchan BranchIslamic Azad UniversityQuchanIran

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