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Extraction and determination of three benzodiazepines in aqueous and biological samples by air-assisted liquid–liquid microextraction and high-performance liquid chromatography

  • Mehdi Ghadi
  • Mohammad Reza HadjmohammadiEmail author
Original Paper
  • 8 Downloads

Abstract

Air-assisted liquid–liquid microextraction (AALLME) as a simple and efficient method was used for extraction of three benzodiazepines (chlordiazepoxide, alprazolam, and lorazepam) in aqueous and biological samples. High-performance liquid chromatography with UV detector was applied for separation and determination of these drugs. To optimize the extraction recovery, effects of seven parameters such as type and volume of extraction solvent, pH and ionic strength of sample solutions, extraction time, centrifugation rate, and time were investigated. Under the optimum condition (chloroform volume as extraction solvent = 300 µL, pH of sample solutions = 7, with 10% w/v salt, number of extraction = 12 cycles, centrifugation rate, and time equal 4000 rpm and 9 min), linearity of the method was from 3 to 10 to 800–1100 µg L−1 for these compounds. Limit of detections was 0.7–2.9 µg L−1. Relative standard deviations for the extraction of 20 and 50 µg L−1 of these benzodiazepines (n = 3) were in the ranges of 1.6–3.1–0.9–2.1%, respectively. This method was successfully applied for extraction and determination of benzodiazepines in different samples such as tap water, tablets, fruit juice, plasma, and urine. Extraction recovery for chlordiazepoxide, alprazolam, and lorazepam was 81.2%, 92.1%, and 90.1%, respectively.

Keywords

Benzodiazepines Chlordiazepoxide Alprazolam Lorazepam Air-assisted liquid–liquid microextraction High-performance liquid chromatography 

Abbreviations

LLE

Liquid–liquid extraction

CPE

Cloud point extraction

SPE

Solid-phase extraction

SPME

Solid-phase microextraction

MAE

Microwave-assisted extraction

HF-LPME

Hallow fiber liquid-phase microextraction

DLLME

Dispersive liquid–liquid microextraction

USAEME

Ultrasound-assisted emulsification microextraction

UA-DLLME

Ultrasound-assisted dispersive liquid–liquid microextraction

AALLME

Air-assisted liquid–liquid microextraction

BZD

Benzodiazepine

ER

Extraction recovery

Notes

Acknowledgements

The authors would like to thank Dr. Abidi Pharmaceutical Co (Tehran, Iran) for supplying BZDs analytical standards.

References

  1. 1.
    M. Uddin. V. Samanidou, I. Papadoyannis, Pharm. Anal. Acta 5, 6 (2014).  https://doi.org/10.4172/2153-2435.1000303 Google Scholar
  2. 2.
    S. Grigoriadis, G. Erlick, Robinson, Ann. Clin. Psychiatry 19, 247 (2007)CrossRefGoogle Scholar
  3. 3.
    A.G. Gilman, T.W. Rall, A.S. Nies, P. Taylor, Goodman and Gilman's the pharmacological basis of therapeutics (Pergamon Press, New York, 1998)Google Scholar
  4. 4.
    O.H. Drummer, Foren. Sci. Int. 142, 101 (2004)CrossRefGoogle Scholar
  5. 5.
    A. El Mahjoub, C. Staub, J Pharm Biomed Anal 23, 447 (2000)CrossRefGoogle Scholar
  6. 6.
    T.L. Laurito, G. de Moraes, G.D. De Nucci, V. Mendes, G. Santagada, M.E.A. Caliendo, J. Mass Spectr. 39, 168 (2004)CrossRefGoogle Scholar
  7. 7.
    K. Langel. T. Gunnar. K. Ariniemi. O. Rajamäki, P. Lillsunde, J. Chromatogr. B 879, 859 (2011)CrossRefGoogle Scholar
  8. 8.
    G. Ugland. M. Krogh, K.E. Rasmussen, J. Chromatogr. B Biomed. Sci. Appl. 749, 85 (2000)CrossRefGoogle Scholar
  9. 9.
    S. Esteve-Romero, M. Carda-Broch, M.-E. Gil-Agustí, D. Capella-Peiró, Bose, TrAc Trends Anal. Chem. 24, 75 (2005)CrossRefGoogle Scholar
  10. 10.
    G. Berzas, M. Castaneda, Pinilla, Talanta 57, 333 (2002)CrossRefGoogle Scholar
  11. 11.
    M. Tomita, T. Okuyama, J. Chromatogr. B Biomed. Sci. Appl. 678, 331 (1996)CrossRefGoogle Scholar
  12. 12.
    W.F. Smyth, Electrophoresis 27 (2006) 2051Google Scholar
  13. 13.
    S. Mitra (2004) Sample preparation techniques in analytical chemistry. Wiley, HobokenGoogle Scholar
  14. 14.
    B. Kinsella. J. O’Mahony., M. Danaher, E. Malone, M. Moloney, H. Cantwell, A. Furey, J. Chromatogr. A 1216, 7977 (2009)CrossRefGoogle Scholar
  15. 15.
    M. Olszowy, P. Szultka, B. Buszewski, R. Kegler, R. Mundkowski, W. Miekisch, J. Schubert, J. Pharm. Biomed. Anal. 53, 1022 (2010)CrossRefGoogle Scholar
  16. 16.
    H.L. Lord, M. Rajabi, S. Safari, J. Pawliszyn, J. Pharm. Biomed. Anal. 44, 506 (2007)CrossRefGoogle Scholar
  17. 17.
    A.B. Tabrizi, M. Harasi, Drug Testing Anal. 4, 145 (2012)CrossRefGoogle Scholar
  18. 18.
    W.F. Smyth, S. McClean, Electrophoresis 19, 2870 (1998)CrossRefGoogle Scholar
  19. 19.
    P. Fernández, P. Cabarcos, C. Vázquez, R. Lorenzo, A. Carro, I. Álvarez, Anal. Bioanal. Chem. 397, 677 (2010)CrossRefGoogle Scholar
  20. 20.
    A. Bugey, C. Staub, J Pharm. Biomed. Anal. 35, 555 (2004)CrossRefGoogle Scholar
  21. 21.
    D.S. Ming, J. Heathcote, J. Chromatogr. B 879, 421 (2011)CrossRefGoogle Scholar
  22. 22.
    K.B. Borges., E.F. Freire. I. Martins, Talanta 78, 233 (2009)CrossRefGoogle Scholar
  23. 23.
    P. Fernández., C. Vázquez., R.A. Lorenzo., A.M. Carro, A.M. Bermejo, Anal. Lett. 43, 1075 (2010).  https://doi.org/10.1080/00032710903492490 CrossRefGoogle Scholar
  24. 24.
    P. Soisungnoen. R. Burakham, S. Srijaranai, Talanta 98, 62 (2012)CrossRefGoogle Scholar
  25. 25.
    Y. ANazaripour., B. Yamini, J. Ebrahimpour, Fasihi, J Separat Sci 39, 2595 (2016)CrossRefGoogle Scholar
  26. 26.
    V. de Bairros. R.M. de Almeida. L. Pantaleão., M. Yonamine, J. Chromatogr. B 975, 24 (2015)CrossRefGoogle Scholar
  27. 27.
    Y.-S. Su, J.-F. Jen, J. Chromatogr. A 1217, 5043 (2010)CrossRefGoogle Scholar
  28. 28.
    L. Miri, F. Jalali, J. Rep. Pharm. Sci. 2, 103 (2013)Google Scholar
  29. 29.
    M.T. Vardini., H.A. Mashayekhi, M. Saber-Tehrani, J. Liq. Chromatogr. Relat. Technol. 35, 988 (2012)CrossRefGoogle Scholar
  30. 30.
    M. Saraji, A.A.H. Bidgoli, Anal Bioanal Chem 397, 3107 (2010)CrossRefGoogle Scholar
  31. 31.
    M.T. Vardini., H.A. Mashayekhi, M. Tehrani, J. Chem. Soc. Pakistan 34, 485 (2012). Retrieved from https://inis.iaea.org/search/default.aspx
  32. 32.
    P. Fernández., C. González., M.T. Pena., A.M. Carro, R.A. Lorenzo, Anal. Chim. Acta 767, 88 (2013)CrossRefGoogle Scholar
  33. 33.
    M. Moradi. Y. Yamini. A. Esrafili, S. Seidi, Talanta 82, 1864 (2010)CrossRefGoogle Scholar
  34. 34.
    J. Cheng. Y. Xia., Y. Zhou., F. Guo, G. Chen, Anal. Chim. Acta 701, 86 (2011)CrossRefGoogle Scholar
  35. 35.
    M.A. Farajzadeh, M.R.A. Mogaddam, Anal. Chim. Acta 728, 31 (2012)CrossRefGoogle Scholar
  36. 36.
    M. Rezaee. Y. Assadi., M.-R.M. Hosseini. E. Aghaee. F. Ahmadi, S. Berijani, J. Chromatogr. A 1116, 1 (2006)CrossRefGoogle Scholar
  37. 37.
    L. He. X. Luo. H. Xie. C. Wang. X. Jiang, K. Lu, Anal. Chim. Acta 655, 52 (2009)CrossRefGoogle Scholar
  38. 38.
    X. You. Z. Xing. F. Liu, X. Zhang, Anal. Chim. Acta 875, 54 (2015)CrossRefGoogle Scholar
  39. 39.
    F. Rezaei. Y. Yamini., M. Moradi, B. Daraei, Anal. Chim. Acta 804, 135 (2013)CrossRefGoogle Scholar
  40. 40.
    S. Khodadoust, M. Ghaedi, J. Separat. Sci. 36, 1734 (2013)CrossRefGoogle Scholar
  41. 41.
    L. Mercolini. R. Mandrioli. M. Amore, M.A. Raggi, J. Separat. Sci. 31, 2619 (2008)CrossRefGoogle Scholar

Copyright information

© Iranian Chemical Society 2019

Authors and Affiliations

  1. 1.Department of chemistryUniversity of MazandaranBabolsarIran

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