Iranian Polymer Journal

, Volume 28, Issue 12, pp 1045–1055 | Cite as

Investigation of degradation of polypropylene in soil using an enzymatic additive

  • Jéssica Pereira Pires
  • Gabriela Messias Miranda
  • Gabriela Lagranha de Souza
  • Flávia Fraga
  • Alessandro da Silva Ramos
  • Gabriel Espindola de Araújo
  • Rosane Angélica Ligabue
  • Carla Maria Nunes Azevedo
  • Rogerio Vescia Lourega
  • Jeane Estela Ayres de LimaEmail author
Original Research


Polypropylene (PP) has been widely used industrially in several sectors, mainly in the use of packaging of different products. Thus, this has been accumulated in our environment due to the incorrect disposal and its high resistance toward degradation, causing an array of environmental impacts. With this, one alternative that has been explored to minimize the problems intensified by these residues is the use of pro-degrading additives. Therefore, the aim of this work is to evaluate the degradation process of PP blends in soil using enzymatic additive. The soil degradation experiment was done for 6 months; monthly collected samples were checked for alterations on the material properties during that time. The extent of PP degradation with enzymatic additive was compared to an organic additive by techniques of FTIR, TGA, DSC, carbonyl index (CI), and crystallinity. From the obtained results it was observed that the additives influenced the degradation of PP. In addition, the enzymatic additive caused more significant changes in the CI (increase of 3693%), crystallinity (variation of 18.7%), and structural characteristics, indicating a greater influence on the degradation process in relation to the organic additive. In this way, this work has had an important role in the research and development of biodegradable materials with the aim of minimizing the effects induced by plastic waste in the environment.


Polypropylene Pro-degradant Additive Degradation Enzymatic Organic 



The present work was performed with the support of Coordination of Improvement of Higher Level Personnel—Brazil (CAPES)—Finance Code 001, and Brasilata Company.


  1. 1.
    Bilal M, Adeel M, Rasheed T, Zhao Y, Iqbal HM (2019) Emerging contaminants of high concern and their enzyme-assisted biodegradation – A review. Environ Int 124:336–353PubMedGoogle Scholar
  2. 2.
    Jose J, Nag A, Nando GB (2014) Environmental ageing studies of impact modified waste polypropylene. Iran Polym J 23:619–636Google Scholar
  3. 3.
    ABIPLAST (2018) Perfil 2017 da Indústria brasileira de transformação e reciclagem de material plástico. Indústria Bras Transform e Reciclagem Mater Plástico 1–43Google Scholar
  4. 4.
    Miyazaki K, Arai T, Shibata K, Terano M, Nakatani H (2012) Study on biodegradation mechanism of novel oxo-biodegradable polypropylenes in an aqueous medium. Polym Degrad Stab 97:2177–2184Google Scholar
  5. 5.
    Rosevelt C, Los Huertos M, Garza C, Nevins HM (2013) Marine debris in central California: quantifying type and abundance of beach litter in Monterey Bay, CA. Mar Pollut Bull 71:299–306PubMedGoogle Scholar
  6. 6.
    Achilias DS, Roupakias C, Megalokonomos P, Lappas AA, Antonakou EV (2007) Chemical recycling of plastic wastes made from polyethylene (LDPE and HDPE) and polypropylene (PP). J Hazard Mater 149:536–542PubMedGoogle Scholar
  7. 7.
    Lazarevic D, Aoustin E, Buclet N, Brandt N (2010) Plastic waste management in the context of a European recycling society: comparing results and uncertainties in a life cycle perspective. Resour Conserv Recycl 55:246–259Google Scholar
  8. 8.
    Faria AU, Martins-Franchetti SM (2010) Biodegradação de filmes de polipropileno (PP), poli(3-hidroxibutirato) (PHB) e blenda de PP/PHB por microrganismos das águas do Rio Atibaia. Polímeros 20:141–147Google Scholar
  9. 9.
    Wan L, Zhou S, Zhang Y (2019) Parallel advances in improving mechanical properties and accelerating degradation to polylactic acid. Int J Biol Macromol 125:1093–1102PubMedGoogle Scholar
  10. 10.
    Ganapathy K, Ramasamy R, Dhinakarasamy I (2018) Polyhydroxybutyrate production from marine source and its application. Int J Biol Macromol 111:102–108Google Scholar
  11. 11.
    Chen DR, Bei JZ, Wang SG (2000) Polycaprolactone microparticles and their biodegradation. Polym Degrad Stab 67:455–459Google Scholar
  12. 12.
    Ojeda TFM, Dalmolin E, Forte MMC, Jacques RJ, Bento FM, Camargo FA (2009) Abiotic and biotic degradation of oxo-biodegradable polyethylenes. Polym Degrad Stab 94:965–970Google Scholar
  13. 13.
    Rosa D, Penteado D, Calil M (2000) Propriedades térmicas e biodegradabilidade de PCL e PHB em um pool de fungos. Polímeros Ciência e Tecnol 15:75–80Google Scholar
  14. 14.
    Liu X, Gao C, Sangwan P, Yu L, Tong Z (2014) Accelerating the degradation of polyolefins through additives and blending. J Appl Polym Sci 131:9001–9015Google Scholar
  15. 15.
    Contat-Rodrigo L (2013) Thermal characterization of the oxo-degradation of polypropylene containing a pro-oxidant/pro-degradant additive. Polym Degrad Stab 98:2117–2124Google Scholar
  16. 16.
    Miyazaki K, Nakatani H (2009) Preparation of degradable polypropylene by an addition of poly(ethylene oxide) microcapsule containing TiO2. Polym Degrad Stab 94:2114–2120Google Scholar
  17. 17.
    Fontanella S, Bonhomme S, Brusson JM, Pitteri S, Samuel G, Pichon G, Lacoste J, Fromageot D, Lemaire J, Delort AM (2013) Comparison of biodegradability of various polypropylene films containing pro-oxidant additives based on Mn, Mn/Fe or Co. Polym Degrad Stab 98:875–884Google Scholar
  18. 18.
    Thomas NL, Clarke J, McLauchlin AR, Patrick SG (2012) Oxo-degradable plastics: degradation, environmental impact and recycling. Proc Inst Civ Eng Waste Resour Manag 165:133–140Google Scholar
  19. 19.
    Mohamad N, Zainol NS, Rahim FF, Maulod HEA, Rahim TA, Shamsuri SR, Azam MA, Yaakub MY, Abdollah MFB, Manaf MEA (2013) Mechanical and morphological properties of polypropylene/epoxidized natural rubber blends at various mixing ratio. Procedia Eng 68:439–445Google Scholar
  20. 20.
    Barbeş L, Rădulescu C, Stihi C (2014) ATR-FTIR spectrometry characterisation of polymeric materials. Rom Reports Phys 66:765–777Google Scholar
  21. 21.
    Ma TS, Gutterson M (1972) Organic elemental analysis. Anal Chem 44:445–457PubMedGoogle Scholar
  22. 22.
    Tavares LB, Rocha RG, Rosa DS (2017) An organic bioactive pro-oxidant behavior in thermal degradation kinetics of polypropylene films. Iran Polym J 26:273–280Google Scholar
  23. 23.
    Stuart B (2004) Infrared spectroscopy: fundamentals and applications. Wiley, West SussexGoogle Scholar
  24. 24.
    Silverstein RM, Bassler GC (1963) Spectrometric identification of organic compounds. J Med Chem 6:826–827Google Scholar
  25. 25.
    Montagna LS, Forte MMC, Santana RMC (2013) Induced degradation of polypropylene with an organic pro-degradant additive. J Mater Sci Eng A 3:123–131Google Scholar
  26. 26.
    Peixoto J, Silva LP, Krüger RH (2017) Brazilian Cerrado soil reveals an untapped microbial potential for unpretreated polyethylene biodegradation. J Hazard Mater 324:634–644PubMedGoogle Scholar
  27. 27.
    Skariyachan S, Patil AA, Shankar A, Manjunath M, Bachappanavar N, Kiran S (2018) Enhanced polymer degradation of polyethylene and polypropylene by novel thermophilic consortia of Brevibacillus sps. and Aneurinibacillus sp. screened from waste management landfills and sewage treatment plants. Polym Degrad Stab 149:52–68Google Scholar
  28. 28.
    Auta HS, Emenike CU, Jayanthi B, Fauziah SH (2018) Growth kinetics and biodeterioration of polypropylene microplastics by Bacillus sp. and Rhodococcus sp. isolated from mangrove sediment. Mar Pollut Bull 127:15–21PubMedGoogle Scholar
  29. 29.
    Gulmine JV, Janissek PR, Heise HM, Akcelrud L (2003) Degradation profile of polyethylene after artificial accelerated weathering. Polym Degrad Stab 79:385–397Google Scholar
  30. 30.
    Albertsson AC, Andersson SO, Karlsson S (1987) The mechanism of biodegradation of polyethylene. Polym Degrad Stab 18:73–87Google Scholar
  31. 31.
    Das MP, Kumar S (2015) An approach to low-density polyethylene biodegradation by Bacillus amyloliquefaciens. 3 Biotech 5:81–86PubMedGoogle Scholar
  32. 32.
    Fletcher M (1996) Bacterial adhesion: molecular and ecological diversity. Willey, New YorkGoogle Scholar
  33. 33.
    Neu TR (1996) Significance of bacterial surface-active compounds in interaction of bacteria with interfaces. Am Soc Microbiol 60:151–166Google Scholar
  34. 34.
    Gu JD (2003) Microbiological deterioration and degradation of synthetic polymeric materials: recent research advances. Int Biodeterior Biodegrad 52:69–91Google Scholar
  35. 35.
    Matsunaga M, Whitney PJ (2000) Surface changes brought about by corona discharge treatment of polyethylene film and the effect on subsequent microbial colonisation. Polym Degrad Stab 70:325–332Google Scholar
  36. 36.
    Husarova L, Machovsky M, Gerych P, Houser J, Koutny M (2010) Aerobic biodegradation of calcium carbonate filled polyethylene film containing pro-oxidant additives. Polym Degrad Stab 95:1794–1799Google Scholar
  37. 37.
    Sivan A (2011) New perspectives in plastic biodegradation. Curr Opin Biotechnol 22:422–426PubMedGoogle Scholar
  38. 38.
    Arkatkar A, Arutchelvi J, Sudhakar M, Bhaduri S, Uppara PV, Doble M (2009) Approaches to enhance the biodegradation of polyolefins. Open Environ Eng J 2:68–80Google Scholar
  39. 39.
    Chawla S, Ghosh AK, Ahmad S, Avasthi DK (2006) Swift heavy ion induced structural and chemical changes in BOPP film. Nucl Instruments Methods Phys Res Sect B Beam Interact Mater Atoms 244:248–251Google Scholar
  40. 40.
    Cadenato A, Ramis X, Salla JM, Morancho JM, Contat-Rodrigo L, Vallés-Lluch A, Ribes-Greus A (2006) Calorimetric studies of PP/Mater-Bi blends aged in soil. J Appl Polym Sci 100:3446–3453Google Scholar
  41. 41.
    Sheik S, Chandrashekar KR, Swaroop K, Somashekarappa HM (2015) Biodegradation of gamma irradiated low density polyethylene and polypropylene by endophytic fungi. Int Biodeterior Biodegrad 105:21–29Google Scholar
  42. 42.
    Longo C, Savaris M, Zeni M, Brandalise RN, Grisa AMC (2011) Degradation study of polypropylene (PP) and bioriented polypropylene (BOPP) in the environment. Mater Res 14:442–448Google Scholar
  43. 43.
    Rivaton A, Gardette JL, Mailhot B, Morlat-Therlas S (2005) Basic aspects of polymer pegradation. Macromol Symp 225:129–146Google Scholar
  44. 44.
    Ramos M, Jiménez A, Peltzer M, Garrigós MC (2012) Characterization and antimicrobial activity studies of polypropylene films with carvacrol and thymol for active packaging. J Food Eng 109:513–519Google Scholar
  45. 45.
    Persico P, Ambrogi V, Carfagna C, Cerruti P, Ferrocino I, Mauriello G (2009) Nanocomposite polymer films containing carvacrol for antimicrobial active packaging. Polym Eng Sci 49:1447–1455Google Scholar
  46. 46.
    Valle MLM, Guimarães MJOC (2004) Degradação de poliolefinas utilizando catalisadores zeolíticos. Polímeros Ciência e Tecnol 14:17–21Google Scholar

Copyright information

© Iran Polymer and Petrochemical Institute 2019

Authors and Affiliations

  • Jéssica Pereira Pires
    • 1
    • 2
  • Gabriela Messias Miranda
    • 2
    • 3
  • Gabriela Lagranha de Souza
    • 2
  • Flávia Fraga
    • 2
  • Alessandro da Silva Ramos
    • 1
    • 3
  • Gabriel Espindola de Araújo
    • 2
  • Rosane Angélica Ligabue
    • 2
    • 3
  • Carla Maria Nunes Azevedo
    • 2
  • Rogerio Vescia Lourega
    • 1
    • 2
    • 3
  • Jeane Estela Ayres de Lima
    • 1
    • 2
    • 3
    Email author
  1. 1.Institute of Petroleum and Natural ResourcesPontifical Catholic University of Rio Grande do SulPorto AlegreBrazil
  2. 2.Polytechnic SchoolPontifical Catholic University of Rio Grande do SulPorto AlegreBrazil
  3. 3.Graduate Program in Materials Engineering and Technology, Polytechnic SchoolPontifical Catholic University of Rio Grande do SulPorto AlegreBrazil

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