Pre-clinical investigation of anti-diarrheal and CNS depressant effect of Wedelia trilobata in Swiss albino mice
- 38 Downloads
Wedelia trilobata (WT) has been used in folk medicine to alleviate several diseases such as cough, cold, flu, fever and inflammation. The study was investigated to evaluate anti-diarrheal and CNS effect of ethanolic extract of W. trilobata in mice. Anti-diarrheal effect was evaluated using castor oil and MgSO4 induced diarrhea tests in mice. Gastrointestinal motility was measured using BaSO4. CNS effect was investigated using hole board, hole cross and elevated plus maze tests. Extract was used at dose of 1000 mg/kg and 2000 mg/kg body weight. Loperamide (10 mg/kg per oral) and diazepam (4 mg/kg) were used as standard drug. The crude extract exhibited significant anti-diarrheal effect in the total number of feces and episodes of diarrheal feces. Treatment with WT at both the doses, very highly reduced diarrhea significantly (p < 0.001) in castor oil and MgSO4 induced diarrheal tests. WT at 1000 mg/kg and 2000 mg/kg reduced diarrheal stools by 71.25% (p < 0.001) and 76.25% (p < 0.001) in castor oil induced diarrhea. In the MgSO4 induced diarrhea, the extract reduced diarrhea by 62.79% (p < 0.001) and 65.12% (p < 0.001), respectively. WT at 2000 mg/kg inhibited GI motility very highly significantly (p < 0.001). Standard drug, loperamide (10 mg/kg) had very highly significant (p < 0.001) antidiarrheal effect. In the CNS, the extract resulted significant depressant effect (p < 0.001) in hole board, hole cross and elevated plus maze test. WT extract possessed significant antidiarrheal and CNS depressant properties.
KeywordsWedelia trilobata Antidiarrheal effect Castor oil and MgSO4 induced diarrhea BaSO4 induced GI motility CNS depressant effect
The authors are thankful to Mr. Shafiqul Islam, Laboratory of Pharmacology and Toxicology, Department of Pharmacy, Jahangirnagar University, for his cooperation in caring of Swiss albino mice during the experiment.
Compliance with ethical standards
This study is reviewed and approved by the Biosafety, Biosecurity and Ethical committee of Jahangirnagar University and the reference number is BBEC, Ju/M 2018(10)2.
Conflict of interest
Md. Al Foyjul Islam has no conflict of interest. Anindya Barua has no conflict of interest. Maksud Hassan Binoy has no conflict of interest. Md. Jahir Alam has no conflict of interest. Md. Ershad has no conflict of interest. M. S. K. Choudhuri has no conflict of interest. Runa Masuma has no conflict of interest.
- Bafna PA, Sarin RV (2012) Herbal antidiarrheals; a review. Int J Res Pharm Biomed Sci 3(2):637–649Google Scholar
- Balekar N, Nakpheng T, Srichana T (2014) Wedelia trilobata L.: a phytochemical and pharmacological review. Chiang Mai J Sci 41(3):590–605Google Scholar
- Ghani A (2003) Medicinal plants of Bangladesh, vol 31(418), 2nd edn. The Asiatic Society of Bangladesh, Dhaka, pp 500–504Google Scholar
- Ghosh A (2014) Survey of ethno-medicinal Climbing plants in Andaman and Nicobar Islands, India. Int J Pharm Life Sci 5(7):3671–3677Google Scholar
- Invasive Species Compendium (2015) Sphagneticola trilobata (Wedelia). http://www.cabi.org/isc/datasheet/56714. Accessed March 12 2015
- Khatun MH, Islam MR, Mamun A, Nahar L, Luthfunnesa Islam MAU (2011) In Vivo Evaluation of CNS depressant and antinociceptive activities of methanol extract of Hibiscus sabdariffa fruits. J Appl Sci Res 7(6):798–804Google Scholar
- Komal S, Kumar S, Rana A (2013) Herbal approaches for diarrhea: a review. IRJP 4:31–38Google Scholar
- Lans C (1996) Ethnoveterinary practices used by livestock keepers in Trinidad and Tobago. In: Unpublished M.Sc. thesis Wageningen: Agricultural University, Department of Ecological Agriculture, NetherlandsGoogle Scholar
- Marie A, Chisholm-Burns MA (2016) Constipation, diarrhea, and irritable bowel syndrome. In: Chisholm-Burns MA, Wells BG, Schwinghammer TL, Malone PM, Kolesar JM, DiPiro JP (eds) Pharmacotherapy principles and practice. McGraw-Hill, New York, pp 333–347Google Scholar
- Pasricha PJ (2006) Treatment of disorder of bowel motility and water flux; anti-emetics; agents used in biliary and pancreatic disease. Part one, Ch-37. In: Goodman & Gillman’s the pharmacological basis of therapeutics, 11th Edn. MacGraw-Hill, New York, p 992Google Scholar
- Pathak K, Das RJ (2013) Herbal medicine-a rational approach in health care system. Int J Ayurvedic Herbal Med 1:86–89Google Scholar
- Sharma DK, Gupta VK, Kumar S, Joshi V, Mandal RSK, Prakash AGB, Singh M (2015) Evaluation of antidiarrheal activity of ethanolic extract of Holarrhena antidysenterica seeds in rats. Vet World. EISSN: 2231-0916Google Scholar
- Shengji P (2001) Ethnobotanical approaches of traditional medicine studies: some experiences from Asia. Pharm Bot 39:74–79Google Scholar
- Victoria C, Bryce J, Fontaine O, Monasc R (2000) Reducing deaths from diarrhea through oral rehydrationtherapy. Bull World Health Org 78(1):1246–1255Google Scholar
- Weeds of Australia (2011) Singapore daisy Sphagneticola trilobata. http://keyserver.lucidcentral.org. Accessed June 15 2015
- Wu ML, Zhang DZ (2008) Progress of researches on the invasive plant Wedelia trilabata. Pharm Today 6:21–23Google Scholar