In-vitro antioxidant and antimicrobial activities of some varieties citrus grown in Algeria

  • Nacera HaraouiEmail author
  • Rachida Allem
  • Tarik Mohammed Chaouche
  • Ahmed Belouazni
Research Article


Polyphenols are scientifically researched molecules because of their multiple biological activities. This study aims to compare the phenolic content and estimate the antioxidant and anti-microbial activities of leaf extracts and juice of ten varieties Citrus fruits grown in Algeria. Antioxidant properties were evaluated by DPPH radical scavenging activity, β-carotene-linoleic acid system and ferric reducing antioxidant capacity. Antimicrobial activity was carried out using agar well diffusion method. The results showed the highest levels of phenolic compounds in juice and leaves of different part and varieties of plant (*P < 0.05). Among all the varieties, C. maxima and C. aurantium presents the highest activity for the three methods cited previously (94.44–91.66% and 95.41–83.84%). On the other hand, antibacterial activity present on the plates was indicated by an inhibition zone surrounding the well containing the juice. The C. Limon and C. aurantium varieties exhibited the highest antimicrobial activity, with inhibition zone ranging from (27.66 ± 0.58, 24.66 ± 1.53 mm) followed by grapefruit and mandarin with areas between (23.00 ± 1.00, 17.66 ± 0.58 mm) on the isolates used. In this study, whatever the parts of citrus (juice and leaves) have been found to contain higher amounts of phenolics and their efficiency as potent antioxidants and antimicrobial properties was confirmed.


Antioxidant activity Antimicrobial activity Phenolic compounds Citrus Juice Leaves 



This research was supported by Laboratory of Natural Products LAPRONA, Abou Bakr Belkaid University, Tlemcen, Algeria, Natural Local Bio-resources Laboratory, Hassiba Benbouali University of Chlef and the Ministry of Higher Education and Scientific Research by their financial support.

Compliance with ethical standards

Ethical statement

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

Nacera Haraoui has no conflict of interest. Rachida Allem has no conflict of interest. Tarik Mohammed Chaouche has no conflict of interest. Ahmed Belouazni has no conflict of interest.


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Copyright information

© Institute of Korean Medicine, Kyung Hee University 2019

Authors and Affiliations

  1. 1.Laboratory of Natural Products, LAPRONAAbouBakr Belkaid UniversityTlemcenAlgeria
  2. 2.Laboratory of Natural Local Bio-Resources, Department of Biology, Faculty of ScienceHassiba Benbouali UniversityChlefAlgeria
  3. 3.Laboratory of Agricultural Production and Sustainable Valorization of Natural Resources (PRAVDURN)University Djilali BounaamaKhemis MilianaAlgeria

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