Determination of biological activities of essential oil and extract obtained from Achillea coarctata Poir.
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In this study evaluated the phenolic, flavonoid contents, antioxidant, antimicrobial and cytotoxic activities of Achillea coarctata extract. Also, chemical compositions of the extract and essential oil were analyzed by HPLC and GC–MS. The antioxidant activity of the extract was determined with phosphomolybdenum, DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging), hydrogen peroxide scavenging, β-carotene bleaching activity, Fe+2-ion reducing power (FRAP) and cupric ion (Cu2+) reducing antioxidant capacity (CUPRAC) assays. The extract and essential oil were investigated for antimicrobial activities against 15 species of microorganisms. The extract has been only found to be effective against Klebsiella pneumoniae, while essential oil was effective against Listeria monocytogenes, K. pneumoniae, Bacillus cereus, Streptococcus pneumoniae and Candida albicans. The cytotoxic activity of the extract on MCF7 (human breast cancer cell line, ATCC HTB-22) and NIH/3T3 (mouse embryo fibroblast cells, ATCC CRL-1658) was studied by MTT assay. The extract showed cytotoxic activity on MCF-7 cell line.
KeywordsAchillea coarctata Antimicrobial activity Antioxidant activity Phenolic compound Cytotoxicity
This work was supported by the Research Fund of the University of Erciyes. Project number is FYL-2017-7077. The authors wish to thank the Anadolu University, Plant, Drug and Scientific Research Center (AUBIBAM) for cytotoxicity analysis.
Compliance with ethical standards
This article does not contain any studies with human participants or animals performed by any of the authors.
Conflict of interest
Sevil Albayrak has no conflict of interest. Nurcan Silahtarlıoğlu has no conflict of interest.
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