Inhibitory effect of effective fraction of Salvia officinalis on aldose reductase activity: strategy to reduce complications of type 2 diabetes
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Aldose reductase (AR) is the key enzyme of the polyol pathway, which plays an important role in the pathogenesis of diabetic complications. AR inhibitors can be used as an important strategy in the treatment of diabetic complications. The aim of the present study was to investigate effect of different fractions of Salvia officinalis on the bovine lens aldose reductase activity. For this purpose, the phenolic and flavonoid contents, IC50 values of different fractions of the S. officinalis to neutralize the DPPH free radicals were first measured. Then, attempts were made to investigate the effect of these fractions on the AR enzyme activity. Results indicated that ethyl acetate fraction had the highest of phenolic and flavonoid contents by 412.6 ± 1.55 and 372.5 ± 6.47 mg/ml, respectively. Also, the ethyl acetate fraction showed the lowest IC50 content of 1.18 μg/ml for scavenging of the free radicals and 9.25 μg/ml for the inhibition of AR activity. According to the Lineweaver–Burk plot, the ethyl acetate fraction acts as an uncompetitive enzyme inhibitor. These findings revealed that all fractions showed inhibitory effect on AR activity, where in ethyl acetate fraction it was found to be maximum which may be due to its high phenolic and flavonoid content.
KeywordsAldose reductase Diabetic complications Salvia officinalis Ethyl acetate fraction
The authors appreciate the joint financial support of this investigation by the research Council of Lorestan University.
Compliance with ethical standards
Conflict of interest
This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-authors.
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