Comparative study of anti-allergic activity of two poly-herbal formulations in ova-challenged allergic rhinitis mice model

  • Richa Srivastava
  • Sajal SrivastavaEmail author
  • Satya Prakash Singh
Research Article


This study was to prepare a novel in situ gel system for nasal delivery of poly-herbal extracts and study its efficacy on the allergic rhinitis model. The main purpose is to compare the efficacy of poly-herbal nasal drops and in situ gel. A temperature depended in situ gel was developed and characterized with gellan gum as a carrier. The system was stable kept at 40 ± 2 °C for 6 months, and the micrographic results showed that in situ gel was safety without mucous irritation when given daily for 26 days to mice with allergic rhinitis that the effect of drops for the treatment. Extracts in gellan gum produced obviously effect on allergic rhinitis at the doses of 20 g/body following intranasal administration, and the efficacy was significantly superior to that of the drops (p < 0.01). This study uses Kruskal–Wallis test analysis of variance (ANOVA) followed by Dunn’s test for the first time to develop, optimize the formulation and assess factors affecting the critical quality attributes. Histopathological study of the nasal mucosa suggested that the formulation was safe for nasal administration. The statistical difference in absolute bioavailability between drop and in situ gel by nasal route suggested that in situ gel had almost 21% increase in bioavailability for Moringa oleifera and for Embelia Ribes in combination there was a 16% increase over drop formulations. Thus the situ gel system is a promising approach for the intranasal delivery of polyherbal extracts for the therapeutic effect improvement.

Graphical abstract


Moringa olifera Embelia ribes Gellan gum Pluronics In-situ Allergic rhinitis Polyherbal 



All the studies were performed at Amity Institute of Pharmacy, Amity University Lucknow campus Uttar Pradesh. We are highly thankful to the institute for proving us the platform to conduct our studies.

Compliance with ethical standards

Ethical statement

This article does not contain any studies with human participants performed by any of the authors. Animal handling and experimental procedures were performed according to the guideline for Care and Use of Animals Laboratory (No: AIP/IAEC/06/2017) was approved by the Institutional Animal Ethics Committee.

Conflict of interest

This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-authors.


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Copyright information

© Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Richa Srivastava
    • 1
  • Sajal Srivastava
    • 1
    Email author
  • Satya Prakash Singh
    • 2
  1. 1.Amity Institute of PharmacyAmity University Uttar Pradesh Lucknow campusLucknowIndia
  2. 2.Pharmacy DepartmentIntegral UniversityLucknowIndia

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