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Methanol extracts from Delonix regia leaves modulate apoptosis in cisplatin-induced nephrotoxicity in male rats

  • O. O. Ojo
  • O. S. Adegbite
  • M. O. Kesinro
  • A. K. Womiloju
  • O. I. Oluyomi
Research Article
  • 16 Downloads

Abstract

The incidence and prevalence of kidney failure has been on the increase in recent times and in addressing the global burden, the present study investigated the influence of methanol extract of Delonix regia (DR) leaves on rat models intraperitoneally injected with a single dose of 5 mg/kg cisplatin to induce nephrotoxicity. Cisplatin-induced nephrotoxic rats received daily oral gavage of either DR of 20 mg/kg, 50 mg/kg or 100 mg/kg for 10 days. On day 10, the levels of kidney mitochondrial ATPase (mATPase), lipid peroxidation (LPO), blood urea nitrogen (BUN) and plasma creatinine (CRE) were determined by spectrophotometry. The presence of cytochrome c and caspase 3 were determined immuohistochemically. Treatment with 20, 50 and 100 mg/kg showed a statistically significant reduction in the levels of BUN and CRE (p < 0.050). The enhanced mATPase and LPO activities in cisplatin-induced nephrotoxic rats were significantly reduced in the protective order 100 > 50 > 20 mg/kg respectively (p < 0.05). The excessive release of cytochrome c and activation of caspase 3 observed in cisplatin-induced nephrotoxic rats were reduced by all treatment groups. The histologic findings revealed that cisplatin-induced nephrotoxic rats had disseminated infiltration of the renal cortex by inflammatory cells while treatment showed regenerated tubules. The suppression of mATPase activity, reduced release of cytochrome c and caspase 3 activation by Delonix regia could be a mechanism at least in part for its use in folklore medicine in the treatment of kidney disease.

Keywords

ATP synthase Apoptosis Delonix regia Oxidative phosphorylation 

Notes

Acknowledgements

We acknowledge the contributions of Mr. R.O. Malomo, the Biochemistry Laboratory technologist, Crawford University, Ogun State, Nigeria.

Compliance with ethical standards

Ethical statement

All procedures performed in studies involving animals were in accordance with the ethical standards of the Institution (Biological Sciences Department, Crawford University, Igbesa, Ogun State, Nigeria).

Conflict of interest

This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-authors.

References

  1. Abulude FO, Adejayan AW (2017) Nutritional values of flamboyant (Delonix regia) seeds obtained in Akure, Nigeria. Peer J Preprint.  https://doi.org/10.7228/peerjpreprints.3113v2 CrossRefGoogle Scholar
  2. Al-Kahtani MA, Abdel-Moneim AM, Elmenshawy OM, El-Kersh MA (2014) Hemin attenuates cisplatin-induced acute renal injury in male rats. Oxidative Med Cell Longev.  https://doi.org/10.1155/2014/476430 CrossRefGoogle Scholar
  3. Bassir O (1963) Plasma inorganic phosphate. Handbook of practical Biochemistry. University of Ibadan, Ibadan, p 55Google Scholar
  4. Bonora MO, Pinton P (2014) The mitochondrial permeability transition pore and cancer. molecular mechanisms involved in cell death. Front Oncol 4:302.  https://doi.org/10.3389/fonc.2014.2014.00302 CrossRefPubMedPubMedCentralGoogle Scholar
  5. Bonora M, Wieckowski MR, Chinopoulos C, Kepp O, Kroemer G et al (2015) Molecular mechanisms of cell death: central implication of ATP synthase in mitochondrial permeability transition. Oncogene 34(12):1475–1486.  https://doi.org/10.1038/onc.2014.96 CrossRefPubMedGoogle Scholar
  6. Campanella M, Casswell E, Chong S, Farah Z et al (2008) Regulation of mitochondrial structure and function by F1F0ATPase inhibitor protein, IFI. Cell Metab 8(1):13–25.  https://doi.org/10.1016/jcmet.2008.06.001 CrossRefPubMedGoogle Scholar
  7. Chen AY, Chen YC (2013) A review of the dietary flavonoid, kaempferol on human health and cancer prevention. Food Chem 138(4):2099–2107.  https://doi.org/10.1016/j.PMCID:PMC3601579 CrossRefPubMedGoogle Scholar
  8. Dakik P, Titorenko VI (2016) Communications between mitochondria, the nucleus, vacuoles, peroxisomes, the endoplasmic reticulum, the plasma membrane, lipid droplets, and the cytosol during yeast chronological aging. Front Genet 7:117.  https://doi.org/10.3389/fgene.2016.00117 CrossRefGoogle Scholar
  9. Evans WC, Trease GE (1999) Trease and Evans pharmacognosy. W.B. Saunders Company Limited, New York.  https://doi.org/10.1111/j.2024-7166.1999.tb0 CrossRefGoogle Scholar
  10. Galluzzi L, Bravo-San Pedro JM, Vitale I, Aaronson SA, Abrams JM et al (2015) Essential versus accessory aspects of cell death: recommendations of the NCCD 2015. Cell Death Differ 22(1):58–73.  https://doi.org/10.1038/cdd.2014.137 CrossRefPubMedGoogle Scholar
  11. Karasawa T, Steyger PS (2015) An integrated view of cisplatin-induced nephrotoxicity and ototoxicity. Toxicol Lett 237(3):219–227.  https://doi.org/10.1016/j.toxlet.2015.06.012 CrossRefPubMedPubMedCentralGoogle Scholar
  12. Linkerman A, Skouta R, Himmerkus N, Mulay SR, Dewitz C et al (2014) Synchronized renal tubular cell death involves ferroptosis. PNAS 111(47):16836–16841.  https://doi.org/10.1073/pnas.1415518111 CrossRefGoogle Scholar
  13. Lowry OH, Rosebrough HJ, Farr AL, Randal RJ (1951) Protein measurement with Folin-phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  14. Mariajancyrani J, Chandramohan G, Ravikumar R (2013) Isolation and identification of phytoconstituents from Delonix regia leaves. Int J Pharm Pharm Sci 5:S4Google Scholar
  15. Mills KT, Xu Y, Zhang W, Bundy JD, Chen CS et al (2015) A systematic analysis of worldwide population-based data on the global burden of chronic kidney disease in 2010. Kidney Int 88(5):950–957.  https://doi.org/10.1038/ki.2015.230 CrossRefPubMedPubMedCentralGoogle Scholar
  16. O’Grady S, Finn SP, Cuffe S, Richard DJ, O’Byrne KJ et al (2014) The role of DNA repair pathways in cisplatin resistant lung cancer. Cancer Treat Rev 40(10):1161–1170.  https://doi.org/10.1016/j.ctrv.2014.10.003 CrossRefPubMedGoogle Scholar
  17. Ojo A (2014) Addressing the global burden of chronic kidney disease through clinical and translational research. Trans Am Clin Climatol Assoc 125:229–246.  https://doi.org/10.1371/journal.pone.0111403 CrossRefPubMedPubMedCentralGoogle Scholar
  18. Olorunsogo OO, Bababunmi EA (1980) Inhibition of succinate-linked reduction of pyridine nucleotide in rat liver mitochondria ‘in vivo’by N-(phosphonomethyl) glycine. Toxicol Lett 7(2):149–152.  https://doi.org/10.1016/0378-4274(80)900048-X CrossRefPubMedGoogle Scholar
  19. Oyedeji OA, Azeez LA, Osifade BG (2017) Chemical and nutritional compositions of flame of forest (Delonix regia) seeds and seed oil. S Afr J Chem 70:16–20CrossRefGoogle Scholar
  20. Prabhu VV, Kaman N, Guruvayoorappan C (2013) 1,2 Diazole prevents cisplatin-induced nephrotoxicity in experimental rats. Pharmacol Rep 65:980–990.  https://doi.org/10.1016/s1734-1140(13)71079-X CrossRefPubMedGoogle Scholar
  21. Rahman M, Hasan N, Das AK, Hossain T, Jahan R, Khatun A, Rahmatullah M (2011) Effect of Delonix regia leaf extract on glucose tolerance in glucose-induced hyperglycemic mice. Afr J Tradit Complement Altern Med 8(1):34–36PubMedGoogle Scholar
  22. Ravindra P, Bhiwgade DA, Kulkarni S, Rataboli PN, Dhume CY (2010) Cisplatin induced histological changes in renal tissue of rat. J Cell Anim Biol 4(7):108–111Google Scholar
  23. Ruberto G, Baratta MT, Deans SG, Dorman HJD et al (2000) Antioxidant and antimicrobial activity of Foeniculum vulgare and Crithmum maritimum essential oils. Planta Med 66:687–693.  https://doi.org/10.1055/s-2000-9773 CrossRefPubMedGoogle Scholar
  24. Schneider WC, Hogeboom GH, Ross HE (1950) Intracellular distribution of enzyme IV. The distribution of nucleic acids and adenosine triphosphatase in normal liver and mouse hepatoma. J Nat Cancer Inst 10(4):977–982Google Scholar
  25. Shinke H, Masuda S, Togashi Y, Ikemi Y, Ozawa A et al (2015) Urinary kidney injury molecule-1 and monocyte chemotactic protein-1 are noninvasive biomarkers of cisplatin-induced nephrotoxicity in lung cancer patients. Cancer Chemother Pharmacol 76(5):989–996.  https://doi.org/10.1007/s00280-y CrossRefPubMedPubMedCentralGoogle Scholar
  26. Slater EC (1953) Mechanism of phosphorylation in the respiratory chain. Nature 172(4387):975.  https://doi.org/10.1038/172975a0 CrossRefPubMedGoogle Scholar
  27. Tohamy HA, Abdella EM, Ahmed RR, Ahmed YA (2014) Assessment of anti-mutagenic, anti-histopathologic and antioxidant capacities of Egyptian bee pollen and propolis extract. Cytotechnology 66(2):283–297.  https://doi.org/10.1007/s10616-013-9568-0 CrossRefPubMedGoogle Scholar
  28. Ulasi II, Ijoma CK (2010) The enormity of chronic kidney disease in Nigeria: the situation in a teaching hospital in South-East Nigeria. J Trop Med.  https://doi.org/10.1155/2010/501957 CrossRefPubMedPubMedCentralGoogle Scholar
  29. Wang LS, Lee CT, Su WL, Huang SC, Wang SC (2016) Delonix regia leaf extract (DRLE): a potential therapeutic agent for cardioprotection. PLoS ONE 11(12):e0167768.  https://doi.org/10.1371/journal.pone.0167768 CrossRefPubMedPubMedCentralGoogle Scholar
  30. Zhan M, Brooks C, Liu F, Sun L, Dong I (2013) Mitochondrial dynamics: regulatory mechanisms and emerging role in renal pathophysiology. Kidney Int 83(4):568–581.  https://doi.org/10.1038/ki.2012.441 CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • O. O. Ojo
    • 1
  • O. S. Adegbite
    • 2
  • M. O. Kesinro
    • 3
  • A. K. Womiloju
    • 3
  • O. I. Oluyomi
    • 3
  1. 1.Department of Chemical SciencesAnchor UniversityAyobo, LagosNigeria
  2. 2.Department of BiochemistryOsun State UniversityOsogboNigeria
  3. 3.Department of Biological SciencesOsun State UniversityOsogboNigeria

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