Overexpressed CYP450 mediated apoptosis evaluates cytotoxicity and teratotoxicity of Cucumis callosus
- 59 Downloads
Fruits of Cucumis callosus (Rottl.) Cogn. (Family: Cucurbitaceae) plant, are commonly known as “bitter cucumber” (English) and “Kachri” (Hindi) in India and have been traditionally used for antioxidant, rich source of vitamin C, antidiabetic and anticancer actions. Tribal peoples of Odissa and West-Bengal are using these fruits during worship and as a vegetable. In vitro cytotoxicity of methanolic seed extract of C. callosus (MSCC) at different concentrations (25, 50, 100, 200, 400) µg/ml and methanolic pericarp extract of C. callosus (MPCC) at (30, 60, 120, 240, 360) µg/ml on Ehrlich Ascites Carcinoma (EAC) cell line, has been evaluated by using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and Trypan blue dye exclusion assay for a period of 3 h treatment. Acute toxicity and in vivo teratotoxicity of both extracts were also evaluated using the Zebrafish embryo developmental assay. The MSCC and MPCC showed the direct cytotoxic effect on EAC cells in a dose-dependent manner with IG50 value 273.17 and 235.08 µg/ml respectively. The MPCC in 240 and 360 µg/ml showed a strong teratotoxic effect on Zebrafish embryo in a dose-dependent manner. The cytotoxicity and teratotoxicity of MPCC were due to overexpressed CYP450 mediated apoptosis in the tissue.
KeywordsCucumis callosus Cytotoxicity Zebrafish Teratotoxicity
We thanks Prof. (Dr.) Malaya Gupta (former professor) and Dr. Pallab Kanti Haldar (Associate Professor) of Jadavpur University, Kolkata for helpful comments on the manuscript. Also, thanks to the authority of “KL Deemed to be University” for providing us all facility for successful completion of research work.
Compliance with ethical standards
All the experiments were conducted in compliance with the guidelines of the Ethics Committee of the International Association for the Study of Pain and with the approval of the Institutional Animal Ethical Committee (IAEC) of University (registration number: 367001/C/CPCSEA).
Conflict of interest
This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-authors.
- Anonymous (1983) Poisoning with a kind of a bitter edible gourd (reported from English (MEDLINE) abstract). Chin J Prev Med 17:29–31Google Scholar
- Bert N, Du L, George H, Mandel E, Leong W (2010) Fundamentals of drug metabolism and drug disposition. In: Mazel P (ed) Laboratory experiments in the study of drug metabolism and drug disposition. PharmaMed Press, Hyderabad, pp 560–575Google Scholar
- Chand T, Bhandari A, Bhupendra KK, Sharma A, Pareek Bansal VK (2012) In vitro antioxidant activity of aqueous extract of seeds of Cucumis callosus (Rottl.) Cogn. Der Pharm Lett 4:840–844Google Scholar
- Gill NS (2014) Review of Cucumis callosus. Glob J Pharm Res 3:1901–1909Google Scholar
- Kirtikar KR, Basu BD (1984) Indian medicinal plants. M/S Periodical Experts, New Delhi, pp 1585–1588Google Scholar
- Rathore M (2009) Nutrient content of important fruit trees from arid zone of Rajasthan. J Hortic For 1:103–108Google Scholar
- Raut M (1959) Glimpses of nature series (No. 4) our monsoon plants. Bombay Natural History Society, BombayGoogle Scholar
- Sajkowska-Kozielewicz JJ, Kozielewicz P, Barnes NM, Wawer I, Paradowska K (2016) Antioxidant, cytotoxic, and cytotoxic activities and total polyphenol contents of the extracts of Geissospermum reticulatum bark. Oxidative medicine and cellular longevity. Hindawi Publishing Corporation, Cairo, pp 1–8Google Scholar
- Seliya AR, Patel NK (2009) Ethnomedicinal uses of climbers from saraswati river region of Patan District, North Gujarat. Ethnobot Leafl 13:865–872Google Scholar
- Thangaraj P (2016) Preliminary Phytochemical Studies. In: Pharmacological assays of plant-based natural products, Progress in drug research, Springer International Publishing, Switzerland, vol 71, pp 15–19.Google Scholar
- Thoennissen NH, Iwanski GB, Doan NB, Okamoto R, Lin P, Abbassi S, Song JH, Yin D, Toh M, Xie WD, Said JW, Koeffler HP (2009) Cucurbitacin B induces apoptosis by inhibition of the JAK/STAT pathway and potentates cytotoxic effects of gemcitabine on pancreatic cancer cells. Cancer Res 69:5876–5884CrossRefGoogle Scholar
- Trivedi PC (2006) Medicinal Plants: Traditional knowledge. In: Sharma UK, Gogoi J (eds) Ethno-medico-botany of some sacred plants of Dhemaji district of Assam. IK International Pvt. Ltd., New Delhi, p 102Google Scholar