Oriental Pharmacy and Experimental Medicine

, Volume 18, Issue 4, pp 365–375 | Cite as

Overexpressed CYP450 mediated apoptosis evaluates cytotoxicity and teratotoxicity of Cucumis callosus

  • Siva Prasad PandaEmail author
  • Bikash Ranjan Jena
  • Gade Kalyani
  • Uttam Prasad Panigrahy
Research Article


Fruits of Cucumis callosus (Rottl.) Cogn. (Family: Cucurbitaceae) plant, are commonly known as “bitter cucumber” (English) and “Kachri” (Hindi) in India and have been traditionally used for antioxidant, rich source of vitamin C, antidiabetic and anticancer actions. Tribal peoples of Odissa and West-Bengal are using these fruits during worship and as a vegetable. In vitro cytotoxicity of methanolic seed extract of C. callosus (MSCC) at different concentrations (25, 50, 100, 200, 400) µg/ml and methanolic pericarp extract of C. callosus (MPCC) at (30, 60, 120, 240, 360) µg/ml on Ehrlich Ascites Carcinoma (EAC) cell line, has been evaluated by using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and Trypan blue dye exclusion assay for a period of 3 h treatment. Acute toxicity and in vivo teratotoxicity of both extracts were also evaluated using the Zebrafish embryo developmental assay. The MSCC and MPCC showed the direct cytotoxic effect on EAC cells in a dose-dependent manner with IG50 value 273.17 and 235.08 µg/ml respectively. The MPCC in 240 and 360 µg/ml showed a strong teratotoxic effect on Zebrafish embryo in a dose-dependent manner. The cytotoxicity and teratotoxicity of MPCC were due to overexpressed CYP450 mediated apoptosis in the tissue.


Cucumis callosus Cytotoxicity Zebrafish Teratotoxicity 



We thanks Prof. (Dr.) Malaya Gupta (former professor) and Dr. Pallab Kanti Haldar (Associate Professor) of Jadavpur University, Kolkata for helpful comments on the manuscript. Also, thanks to the authority of “KL Deemed to be University” for providing us all facility for successful completion of research work.

Compliance with ethical standards

Ethical statement

All the experiments were conducted in compliance with the guidelines of the Ethics Committee of the International Association for the Study of Pain and with the approval of the Institutional Animal Ethical Committee (IAEC) of University (registration number: 367001/C/CPCSEA).

Conflict of interest

This manuscript described has not been published before; not under consideration for publication anywhere else; and has been approved by all co-authors.

Supplementary material

13596_2018_324_MOESM1_ESM.docx (3.3 mb)
Supplementary material 1 (DOCX 3390 kb)


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Copyright information

© Institute of Korean Medicine, Kyung Hee University and Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Siva Prasad Panda
    • 1
    Email author
  • Bikash Ranjan Jena
    • 2
  • Gade Kalyani
    • 1
  • Uttam Prasad Panigrahy
    • 1
  1. 1.KL College of PharmacyKLEF Deemed to be UniversityVaddeswaram, GunturIndia
  2. 2.SIIMS College of PharmacyGunturIndia

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