The age of black pine (Pinus nigra Arn. ssp. salzmannii (Dunal) Franco) mother trees has no effect on seed germination and on offspring seedling performance
Abstract
Key message
We sampled Pinus nigra cones in 29 trees in an age range of 90 to 725 years. The mother tree age did not significantly influence the pinecone or pine seed size, seed germination capacity, or plant size or vigor displayed during the first year of growth in the nursery.
Context
Pinus nigra Arn. ssp. salzmannii is a long-lived Mediterranean species, with millenary trees residing in an old-growth forest in the Cazorla Mountain Range in SE Spain which is home to the oldest known trees in the Iberian Peninsula.
Aims
This study aimed to assess how the mother tree age in Pinus nigra influences seed viability, germination capacity, and the seedling survival and growth during the first year under nursery conditions.
Methods
Twenty-nine trees aged 90 to 725 years were selected and 60 cones were harvested per tree to study the cone characteristics (size and weight), seed viability, and germination capacity in relation to the mother tree age. Eighty germinated seeds per tree were transferred to the nursery and seedling survival and growth were measured after the first growing season.
Results
Significant between-tree differences were detected for cone characteristics (cone and seed weight, number of seeds per cone), as well as for germination capacity. Notably, however, the mother tree age did not significantly influence the aforementioned parameters.
Conclusion
Forest management and regeneration practices of Pinus nigra should take into account that trees of this species up to at least 725 years old produce seeds with a fairly high reproductive capacity.
Keywords
Pinus nigra Aging Seed viability Old-growth forestsNotes
Acknowledgements
We thank Teresa Moro and Valentín Badillo (from the Natural Park Forest Service, Junta de Andalucía, Spain) for providing the sampling permits required for the area and for their interest in the project. The dendrochronological fieldwork for this research was partially funded by the Netherlands Organisation for Scientific Research (NWO-number 236-61-001) and by the Spanish Ministry of Science, Innovation and Universities (Plan I+D+i AGL2017-83828-C2-2-R) and FEDER funds. We thank Antonio Jesús López- Soroche and Juan Manuel Castellano, students at the University of Huelva, Spain; Aldo Carrasco from the University Arturo Prat, Chile; and Ana J. Moreno, Y. Ramírez and Salvador Sarabia, from the University of Chapingo, Mexico, for their help with the lab and nursery work.
Funding
The dendrochronological fieldwork of this research was funded by the Netherlands Organisation for Scientific Research (NWO-number 236-61-001) and National Geographic Society (Waitts Grant W 329-14). The University of Huelva, Spain, provided the lab and nursery with the needed material to perform the experiment.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
Supplementary material
References
- Andivia E, Fernández M, Vázquez-Piqué J (2011) Autumn fertilization of Quercus ilex ssp. ballota (Desf.) Samp.nursery seedlings: effects on morpho-physiology and field performance. Ann ForSci 68(3):543–553. https://doi.org/10.1007/s13595-011-0048-4 CrossRefGoogle Scholar
- Ayari A, Khouja ML (2014) Ecophysiological variables influencing Aleppo pine seed and cone production: a review. Tree Physiol. 34 (4):426–437. https://doi.org/10.1093/treephys/tpu022
- Beadle C, Duff G, Richardson A (2009) Old forests, new management: the conservation and use of old growth forests in the 21stcentury. For Ecol Manag 258(4):339–340CrossRefGoogle Scholar
- Brutovská E, Sámelová A, Dušička J, Mičieta K (2013) Ageing of trees: application of general ageing theories. Ageing Res Rev 12(4):855–866. https://doi.org/10.1016/j.arr.2013.07.001 CrossRefPubMedGoogle Scholar
- Carey EV, Sala A, Keane M, Callaway RM (2001) Are old forests underestimated as global carbon sinks? Glob Change Biol 7(4):339–344CrossRefGoogle Scholar
- Catalán G (1993) Semillas de árboles y arbustos forestales. Ministerio de Agricultura, Pesca y Alimentación. ICONA, Madrid, p 392 ISBN: 84-8014-007-0Google Scholar
- Creus J (1998) A propósito de los árboles más viejos de la España peninsular: los Pinus nigra Arnold subsp. salzmannii (Dunal) Franco de Puertollano-Cabañas Sierra de Cazorla, Jaén. Montes 54:68–76Google Scholar
- Davis AS, Jacobs DF (2005) Quantifying root system quality of nursery seedlings and relationship to outplanting performance. New For 30(2–3):295–311. https://doi.org/10.1007/s11056-005-7480-y CrossRefGoogle Scholar
- De Aranda G (1990) Los Bosques Flotantes: historia de un roble del siglo XVIII. Colección Técnica, Ministerio de Agricultura, Pesca y Alimentación. ICONA, Madrid, p 231 ISBN: 84-85496-50-7Google Scholar
- De la Cruz E (1994) La destrucción de los Montes (claves histórico-jurídicas). Universidad Complutense de Madrid, Madrid, p 287 ISBN: 84-86926-70-XGoogle Scholar
- Derridj A, Abdelli D, Adjaoud D, Asmani A, Fady B, Hedjam H, Larbi-Aidrous N, Zanndouche O, Krouchi F (2011) A synthesis on several years study on Pinus nigra ssp mauretanica in Algeria. In: Abstract Book. Medpine 4: 4th International Conference on Mediterranean Pines. June, 6-10, 2011. Avignon, France O8-2, p 76Google Scholar
- Dominguez-Delmás M, Alejano R, Wazny T, García-González I (2013) Radial growth variations of black pine along an elevation gradient in the Cazorla Mountains (South of Spain) and their relevance for historical and environmental studies. Eur J For Res 132(4):635–652. https://doi.org/10.1007/s10342-013-0700-7 CrossRefGoogle Scholar
- Escudero A, Barrero S, Pita JM (1997) Efects of high temperatures and ash on seed germination of two Iberian pines (Pinus nigras sp salzmannii, P sylvestris var iberica). Ann Sci For 54(6):553–562CrossRefGoogle Scholar
- FAO (1985) A guide to forest seed handling. FAO forestry paper 20/2.Ed. Food and Agriculture Organization of the United Nations, Rome Available at: http://www.fao.org/docrep/006/ad232e/AD232E09.htm. ISBN 92–5–102291-7Google Scholar
- Fernández M, Marcos C, Tapias R, Ruiz F, López G (2007) Nursery fertilization affects the frost-tolerance and plant quality of Eucalyptus globulus Labill. cuttings. Ann. For Sci 64(8):865–873. https://doi.org/10.1051/forest:2007071 CrossRefGoogle Scholar
- Fernández M, Alejano R, Domínguez L, Tapias R (2008) Temperature controls cold hardening more effectively than photoperiod in four Mediterranean broadleaf evergreen species. Tree For Sci Biotechnol 2(1):43–49Google Scholar
- Ganatsas P, Tsakaldimi M, Thanos C (2008) Seed and cone diversity and seed germination of Pinus pinea in Strofylia site of the Natura 2000 network. Biodivers Conserv 17(10):2427–2439. https://doi.org/10.1007/s10531-008-9390-8 CrossRefGoogle Scholar
- Gordo J, Calama R, Pardos M, Bravo F, Montero G, (Eds) (2012) La regeneración natural de los pinares en los arenales de la meseta castellana. In: Instituto Universitario de Investigación en Gestión Forestal Sostenible (Universidad de Valladolid-INIA). Valladolid. 254 p. ISBN: 978-84-615-9823-6Google Scholar
- Guariguata MR, Pinard MA (1998) Ecological knowledge of regeneration from seed in neotropical forest trees: implications for natural forest management. For Ecol Manag 112(1–2):87–99CrossRefGoogle Scholar
- Herrera CM (1991) Dissecting factors responsible for individual variations in plant fecundity. Ecology 72(4):1436–1448CrossRefGoogle Scholar
- Holloway DM, Brook B, Kang JH, Wong C, Wu M (2016) A quantitative study of cotyledon positioning in conifer development. Botany 94(11):1063–1074. https://doi.org/10.1139/cjb-2015-0242 CrossRefGoogle Scholar
- Kitajima K, Fenner M (2000) Ecology of seedling regeneration. Seeds: the ecology of regeneration in Plant Communities. CAB International, Wellington, p 410Google Scholar
- Klekovski EJ, Godfrey PJ (1989) Ageing and mutation in plants. Nature 340:389–391CrossRefGoogle Scholar
- Lanner RM (2002) Why do trees live so long? Ageing Res Rev 1(4):653–671CrossRefGoogle Scholar
- Lanner RM, Connor KF (2001) Does bristlecone pine senesce? Exp Gerontol 36(4–6):675–685CrossRefGoogle Scholar
- Larcher W (2003) Physiological plant ecology: ecophysiology and stress physiology of functional groups. Springer-Verlag, Berlin, p 513CrossRefGoogle Scholar
- Lindenmayer DB, Franklin JF (2002) Conserving forest biodiversity. A Comprehensive Multiscaled Approach. Island Press, Washington DC, p 351Google Scholar
- Lindenmayer DB, McArthy MA (2002) Congruence between natural and human forest disturbance: a case study for Australian montane ash forest. For Ecol Manag 155(1–3):319–335CrossRefGoogle Scholar
- Lucas-Borja ME, Fonseca T, Parresol B, Silva-Santos P, García-Morote FA, Tíscar-Oliver PA (2011) Modelling Spanish black pine seedling emergence: establishing management strategies for endangered forest areas. For Ecol Manag 26(2):195–202. https://doi.org/10.1016/j.foreco.2011.03.023. CrossRefGoogle Scholar
- Lucas-Borja ME, Fonseca TF, Lousada JL, Silva-Santos P, García EM, Abellán MA (2012) Natural regeneration of Spanish black pine [Pinus nigra Arn. ssp. salzmannii (Dunal) Franco] at contrasting altitudes in a Mediterranean mountain área. Ecol Res 27(5):913–921. https://doi.org/10.1007/s11284-012-0969-x. CrossRefGoogle Scholar
- Lucas-Borja ME, Candel-Pérez D, Onkelinx T, Fule PZ, Moya D, Gómez R, de las Heras J (2017) Early Mediterranean pine recruitment in burned and unburned Pinus nigra Arn. ssp. salzmannii stands of Central Spain: influence of species identity, provenances and post-dispersal predation. For Ecol Manag 390:203–211. https://doi.org/10.1016/j.foreco.2017.01.026 CrossRefGoogle Scholar
- Mao P, Han G, Wang G, Yu J, Shao H (2014) Effects of age and stand density of mother trees on early Pinus thunbergii seedling establishment in the coastal zone, China. Sci World J 2014(468036):9. https://doi.org/10.1155/2014/468036 CrossRefGoogle Scholar
- Mencuccini M, Oñate M, Peñuelas J, Rico L, Munné-Bosch S (2014) No signs of meristem senescence in old Scots pine. J Ecol 102(3):555–565. https://doi.org/10.1111/1365-2745.12219 CrossRefGoogle Scholar
- Müller M, Siles L, Cela J, Munné-Bosch S (2014) Perennially young: seed production and quality in controlled and natural populations of Cistus albidus reveal compensatory mechanisms that prevent senescence in terms of seed yield and viability. J Exp Bot 65(1):287–297. https://doi.org/10.1093/jxb/ert372 CrossRefPubMedGoogle Scholar
- Munné-Bosch S (2008) Do perennials really senesce? Trends Plant Sci 13(5):216–220CrossRefGoogle Scholar
- Parker WC, Noland TL, Morneault AE (2006) The effects of seed mass on germination, seedling emergence, and early seedling growth of eastern white pine (Pinus strobus L.). New For 32(1):33–49. https://doi.org/10.1007/s11056-005-3391-1 CrossRefGoogle Scholar
- Ruiz de la Torre J (2006). Mayor F (ed) Organismo Autónomo Parque Naturales. DGCONA. Ministerio de Medio Ambiente. Madrid. Spain. 1756 p. ISBN: 10: 84–8014–660-5Google Scholar
- Sauer M, Robert S, Kleine-Vehn J (2013) Auxin: simply complicated. J Exp Bot 64(9):2565–2577. https://doi.org/10.1093/jxb/ert139 CrossRefPubMedGoogle Scholar
- Serrada R, Domínguez S, Sánchez MI, Ruiz J (1994) El problema de la regeneración natural del Pinus nigra Arn. Montes 36:52–57Google Scholar
- Smith DM, Larson BC, Kelty MJ, Ashton PMS (1997) The practice of silviculture: applied forest ecology. John Wiley and Sons, New York, p 537Google Scholar
- Stephenson NL, Das AJ, Condit R, Russo SE, Baker PJ, Beckman NG, Coomes DA, Lines ER, Morris WK, Rüger N, Álvarez E, Blundo C, Bunyavejchewin S, Chuyong G, Davies SJ, Duque Á, Ewango CN, Flores O, Franklin JF, Grau HR, Hao Z, Harmon ME, Hubbell SP, Kenfack D, Lin Y, Makana JR, Malizia A, Malizia LR, Pabst RJ, Pongpattananurak N, Su SH, Sun IF, Tan S, Thomas D, van Mantgem PJ, Wang X, Wiser SK, Zavala MA (2014) Rate of tree carbon accumulation increases continuously with tree size. Nature 507:90–93. https://doi.org/10.1038/nature12914 CrossRefPubMedGoogle Scholar
- Temel F, Gülcü S, Ölmez Z, Göktürka A (2011) Germination of Anatolian black pine (Pinus nigra subsp. pallasiana) seeds from the lakes region of Turkey: geographic variation and effect of storage. Not Bot Horti Agrobo 39(1):267–274CrossRefGoogle Scholar
- Thomas H (2013) Senescence, ageing and death of the whole plant. New Phytol 197(3):696–711. https://doi.org/10.1111/nph.12047 CrossRefPubMedGoogle Scholar
- Tíscar Oliver PA (2002) Capacidad reproductiva de Pinus nigra subsp. salzmannii en relación con la edad de la planta madre. Invest Agr:Sist Recur For 11(2):357–371Google Scholar
- Tíscar Oliver PA (2007) Dinámica de regeneración de Pinus nigra ssp. salzmannii al sur de su área de distribución: etapas, procesos y factores implicados. Invest Agr: Sist Recur For 16(2):124–135Google Scholar
- Tíscar PA, Linares JC (2011) Pinus nigra subsp. salzmannii forests from Southeast Spain: using structure and process information to guide management. Chapter 11, 27 p. In: Frisiras CT (ed) Pine Forests: types, threats and management. Nova Science Publishers Inc. ISBN: 978-1-61324-493-7Google Scholar
- Tíscar PA, Lucas JC (2010) Seed mass variation, germination time and seedling performance in a population of Pinus nigra subsp. salzmannii. For Syst 19(3):344–353. https://doi.org/10.5424/fs/2010193-9094 CrossRefGoogle Scholar
- Viglas JN, Brown CD, Johnstone JF (2013) Age and size effects on seed productivity of northern black spruce. Can J For Res 43(6):534–543. https://doi.org/10.1139/cjfr-2013-0022 CrossRefGoogle Scholar
- Wahid N, Bounoua L (2013) The relationship between seed weight, germination and biochemical reserves of maritime pine (Pinus pinaster Ait.) in Morocco. New For 44(3):385–397. https://doi.org/10.1007/s11056-012-9348-2 CrossRefGoogle Scholar
- Xu Y, Cail N, He B, Zhang R, Zhao W, Mao J, Duan A, Li Y, Woeste K (2016) Germination and early seedling growth of Pinus densata Mast. provenances. J For Res 27(2):283–294. https://doi.org/10.1007/s11676-015-0186-x CrossRefGoogle Scholar