Plastic response of four maritime pine (Pinus pinaster Aiton) families to controlled soil water deficit
Separating the internal (ontogenetic) and external (environmental) components of maritime pine development during controlled soil water deficit helps to highlight the plastic response. The adjusted measurements reveal significant differences between families for their plastic response for several physiology and growth traits.
Soil water deficit is and will be a growing problem in some regions. Pinus pinaster Ait. is a species of commercial interest and is recognized as a drought-avoiding species. It is thus of interest to evaluate the adaptation potential of P. pinaster to soil water deficit.
This paper aims to estimate the plastic response to the variation of water availability at the family level (half-sibs).
Two-year-old P. pinaster cuttings from four families were submitted during 6 weeks to two contrasting watering regimes. The experiment started in April 2011 shortly after sprouting. The photosynthesis and stomatal conductance to water vapor were measured on 1-year-old needles. Intrinsic water-use efficiency was calculated as the ratio of photosynthesis to stomatal conductance. Radial growth, length of terminal shoot, and total height were also measured. The ontogenetic component of tree development was estimated on the well-watered trees for all the traits. Then, this development effect was eliminated from the data collected on the trees submitted to the soil water deficit in order to keep only the effect of this soil water deficit.
After 6 weeks of reduced watering, the value of all adjusted traits decreased. An average plastic response to the variation of water availability was found to be significant and variable at the family level for the six adjusted variables.
These results suggest that there is genetic variation of phenotypic plasticity to drought in P. pinaster for several traits, including stomatal conductance, which appears to be a promising variable for future selection for resistance to drought.
KeywordsAdaptation Ontogenetic CO2 assimilation Stomatal conductance Water-use efficiency Growth
The authors thank Patrick Poursat, Christophe Borel, and Bernard Lhomel of the experimental unit UE GBFOR, and Frédéric Millier, from the plateau technique GENOBOIS, INRA Val de Loire, Orléans, France, for the installation and the management of the experimental design.
Xunta de Galicia was the owner of the original material from which the cuttings were derived. The cuttings were produced by TRAGSA with funds of Restauración y Gestión Forestal – Bosques del Futuro (PSS-310000-2009-20) project of the Spanish Science and Innovation Ministry. The research was funded by the Region Centre-Val de Loire France Project Xylome no. 2009 0003 8263.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Aranda I, Alía R, Ortega U, Dantas ÂK, Majada J (2010) Intra-specific variability in biomass partitioning and carbon isotopic discrimination under moderate drought stress in seedlings from four Pinus pinaster populations. Tree Genet Genomes 6:169–178. https://doi.org/10.1007/s11295-009-0238-5 CrossRefGoogle Scholar
- Correia I, Almeida MH, Aguiar A, Alia R, David TS, Pereira JS (2008) Variations in growth, survival and carbon isotope composition (13C) among Pinus pinaster populations of different geographic origins. Tree Physiol 28:1545–1552. https://doi.org/10.1093/treephys/28.10.1545 CrossRefPubMedGoogle Scholar
- de Miguel M, Sanchez-Gomez D, Cervera MT, Aranda I (2012) Functional and genetic characterization of gas exchange and intrinsic water use efficiency in a full-sib family of Pinus pinaster Ait. in response to drought. Tree Physiol 32:94–103. https://doi.org/10.1093/treephys/tpr122 CrossRefPubMedGoogle Scholar
- de Miguel M, Cabezas J-A, de María N, Sánchez-Gómez D, Guevara M-Á, Vélez M-D, Sáez-Laguna E, Díaz L-M, Mancha J-A, Barbero M-C, Collada C, Díaz-Sala C, Aranda I, Cervera M-T (2014) Genetic control of functional traits related to photosynthesis and water use efficiency in Pinus pinaster Ait. drought response: integration of genome annotation, allele association and QTL detection for candidate gene identification. BMC Genomics 15:464. https://doi.org/10.1186/1471-2164-15-464 CrossRefPubMedCentralPubMedGoogle Scholar
- DeWitt TJ, Scheiner SM (2004) Phenotypic plasticity: functional and conceptual approaches. Oxford University Press, OxfordGoogle Scholar
- Egea G, GonzáLez-Real MM, Baille A, Nortes PA, Diaz-Espejo A (2011) Disentangling the contributions of ontogeny and water stress to photosynthetic limitations in almond trees: photosynthetic limitations in almond trees. Plant Cell Environ 34:962–979. https://doi.org/10.1111/j.1365-3040.2011.02297.x CrossRefPubMedGoogle Scholar
- Farquhar GD, Sharkey TD (1982) Stomatal conductance and photosynthesis. Annu Rev Plant Physiol 33:317–345. https://doi.org/10.1146/annurev.pp.33.060182.001533 CrossRefGoogle Scholar
- Gaspar MJ, Velasco T, Feito I, Alía R, Majada J (2013) Genetic variation of drought tolerance in Pinus pinaster at three hierarchical levels: a comparison of induced osmotic stress and field testing. PLoS One 8:e79094. https://doi.org/10.1371/journal.pone.0079094 CrossRefPubMedCentralPubMedGoogle Scholar
- Limousin J-M, Misson L, Lavoir A-V, Martin NK, Rambal S (2010) Do photosynthetic limitations of evergreen Quercus ilex leaves change with long-term increased drought severity? Plant Cell Environ. https://doi.org/10.1111/j.1365-3040.2009.02112.x
- Monclus R, Dreyer E, Villar M, Delmotte FM, Delay D, Petit J-M, Barbaroux C, Le Thiec D, Brechet C, Brignolas F (2006) Impact of drought on productivity and water use efficiency in 29 genotypes of Populus deltoides × Populus nigra. New Phytol 169:765–777. https://doi.org/10.1111/j.1469-8137.2005.01630.x CrossRefPubMedGoogle Scholar
- Pachauri RK, Leo M, et Intergovernmental Panel on Climate Change (éds) (2015) Climate Change 2014: Synthesis Report. Intergovernmental Panel on Climate Change, GenevaGoogle Scholar
- Paiva JAP, Garnier-Géré PH, Rodrigues JC, Alves A, Santos S, Graça J, Le Provost G, Chaumeil P, Da Silva-Perez D, Bosc A, Fevereiro P, Plomion C (2008) Plasticity of maritime pine (Pinus pinaster) wood-forming tissues during a growing season. New Phytol 179:1180–1194. https://doi.org/10.1111/j.1469-8137.2008.02536.x CrossRefGoogle Scholar
- Picon C, Guehl JM, Ferhi A (1996) Leaf gas exchange and carbon isotope composition responses to drought in a drought-avoiding (Pinus pinaster) and a drought-tolerant (Quercus petraea) species under present and elevated atmospheric CO2 concentrations. Plant Cell Environ 19:182–190. https://doi.org/10.1111/j.1365-3040.1996.tb00239.x CrossRefGoogle Scholar
- Plomion C, Bartholomé J, Bouffier L, Brendel O, Cochard H, De Miguel M, Delzon S, Gion J-M, Gonzalez-Martinez SC, Guehl J-M, Lagraulet H, Le Provost G, Marguerit E, Porté A (2016) Understanding the genetic bases of adaptation to soil water deficit in trees through the examination of water use efficiency and cavitation resistance: maritime pine as a case study. J Plant Hydraul 3(8):008. https://doi.org/10.20870/jph.2016.e008 CrossRefGoogle Scholar
- R software (version 2.8.0, R 274Q8 Development Core Team 2008)Google Scholar
- Sánchez-Gómez D, Majada J, Alía R, Feito I, Aranda I (2010) Intraspecific variation in growth and allocation patterns in seedlings of Pinus pinaster Ait. submitted to contrasting watering regimes: can water availability explain regional variation? Ann For Sci 67:505–504. https://doi.org/10.1051/forest/2010007 CrossRefGoogle Scholar
- Schlichting CD (1986) The evolution of phenotypic plasticity in plants. Annu Rev Ecol Syst 17:667–693. https://doi.org/10.1146/annurev.es.17.110186.003315 CrossRefGoogle Scholar
- Schulze ED, Turner NC, Gollan T, Shackel KA (1987) Stomatal responses to air humidity and to soil drought. Stomatal Funct 804713472:311–321Google Scholar
- Slaney M (2006) Impact of elevated temperature and [CO2] on spring phenology and photosynthetic recovery of boreal Norway spruce. Southern Swedish Forest research Centre. Swedish University of Agricultural Sciences, AlnarpGoogle Scholar