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Apidologie

pp 1–11 | Cite as

The capping pheromones and putative biosynthetic pathways in worker and drone larvae of honey bees Apis mellifera

  • Qiu-Hong Qin
  • Xu-Jiang He
  • Andrew B. Barron
  • Lei Guo
  • Wu-Jun Jiang
  • Zhi-Jiang ZengEmail author
Original article
  • 41 Downloads

Abstract

In honey bees (Apis mellifera), methyl palmitate (MP), methyl oleate (MO), methyl linoleate (ML), and methyl linolenate (MLN) are important pheromone components of the capping pheromones triggering the capping behavior of worker bees. In this study, we compared the amounts of these four pheromone components in the larvae of workers and drones, prior to be capped, in the process of being capped and had been capped. The amounts of MP, MO, and MLN peaked at the capping larval stage, and ML was highest at capped larvae in worker larvae, whereas in drone larvae, the amounts of the four pheromone components were higher overall and increased with aging. Furthermore, we proposed de novo biosynthetic pathways for MP, MO, ML, and ML, from acetyl-CoA. Besides, stable isotope tracer 13C and deuterium were used to confirm that these capping pheromone components were de novo synthesized by larvae themselves rather than from their diets.

Keywords

honey bee larvae capping pheromones pathways 

Notes

Acknowledgments

We thank Prof. Junwu Ma for help on biosynthetic pathway analysis and Dr. Qiang Huang for revising the manuscript.

Contributions

QHQ, XJH, and WJJ conducted all experiments; ZJJ designed the experiments; QHQ, XJH, and ABB wrote the paper; and LG participated in experiments and data analysis.

Funding information

This work was supported by the National Natural Science Foundation of China (31572469, 31872432) and the Earmarked Fund for China Agriculture Research System (CARS-44-KXJ15).

Supplementary material

13592_2019_686_Fig5_ESM.png (25 kb)
Figure S1.

The correlation coefficients of the four standard curves. (PNG 25 kb)

13592_2019_686_MOESM1_ESM.tif (47 kb)
High resolution image (TIF 47 kb)
13592_2019_686_MOESM2_ESM.xls (29 kb)
Table SI. Primers for qRT-PCR. (XLS 29 kb)
13592_2019_686_MOESM3_ESM.docx (20 kb)
Table SII. Statistics of RNA sequencing in worker and drone larvae. (DOCX 19 kb)
13592_2019_686_MOESM4_ESM.xlsx (15 kb)
Table SIII. Pearson correlation coefficient among three biological replicates of each larval group. (XLSX 15 kb)
13592_2019_686_MOESM5_ESM.docx (17 kb)
Table SIV. The number of significantly differentially expressed genes in larvae of different capping stage. (DOCX 17 kb)
13592_2019_686_MOESM6_ESM.xlsx (27 kb)
Table SV. Expression of genes relating to the biosynthesis of capping pheromone components in larvae of different capping stages. (XLSX 26 kb)

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Copyright information

© INRA, DIB and Springer-Verlag France SAS, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Honeybee Research InstituteJiangxi Agricultural UniversityNanchangPeople’s Republic of China
  2. 2.Guangxi Liuzhou Animal Husbandry and Veterinary SchoolLiuzhouPeople’s Republic of China
  3. 3.Department of Biological SciencesMacquarie UniversityNorth RydeAustralia
  4. 4.Research and Development CentreChina Tobacco Jiangxi Industrial Co., LTD.NanchangPeople’s Republic of China

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