, Volume 50, Issue 4, pp 482–496 | Cite as

Nucleotide sequence variations may be associated with virulence of deformed wing virus

  • Sandra Barroso-ArévaloEmail author
  • Marina Vicente-Rubiano
  • Fernando Molero
  • Francisco Puerta
  • José Manuel Sánchez-Vizcaíno
Original article


Western honey bees (Apis mellifera) are key players in crop pollination and in the maintenance of global biodiversity. Their viability is threatened by Varroa destructor, which acts as a vector of the deformed wing virus (DWV). Several genetic DWV variants have been reported, but it is unclear whether their virulence differs. We examined the prevalence of V. destructor and DWV as well as bee health in two colonies over 21 months and then characterizing DWV variants from each colony using phylogenetics. Colony H showed no signs of disease or mortality, and DWV sequence from this colony clustered with VDV/DWV-B sequences previously reported in healthy colonies. Colony W showed DWV symptoms, and DWV sequence clustered with DWV-A sequences previously reported in colonies with symptoms. These results suggest that nucleotide variations in the DWV genome can affect its virulence. Genotyping DWV variants in colonies may be an effective tool to assess risk and initiate preventive measures early.


deformed wing virus V. destructor virus virulence phylogeny honey bee 



The authors thank Deborah Kukielka for valuable support and help during reviewing of the manuscript.

Author contributions

SB, MVR, and JSV designed experiments, FP helped in the interpretation of the data, SB and FMR performed experiments and analysis, and SB wrote the paper. All authors read and approved the manuscript.

Supplementary material

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Copyright information

© INRA, DIB and Springer-Verlag France SAS, part of Springer Nature 2019

Authors and Affiliations

  1. 1.VISAVET Centre and Animal Health Department, Veterinary SchoolComplutense University of MadridMadridSpain
  2. 2.Apicultural Reference Center in Andalusia (CERA)AndalusiaSpain

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