Onset of foraging and lifespan of Africanized honey bees (Apis mellifera) infected with different levels of Nosema ceranae spores in Neotropical Mexico
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Nosema ceranae is a microsporidium pathogen widely spread around the world. Negative effects on foraging behavior and longevity of EHB colonies have been associated with this pathogen as well as possible population losses, but its effects have not been studied in tropical adapted honey bees. We studied the interaction between this pathogen and Africanized honey bees (AHB) in the Yucatan peninsula of Mexico where N. ceranae has only been detected since 2008. Non-infected and artificially infected workers with two different spore concentrations were introduced in observation hives to evaluate the onset and duration of foraging and longevity. The results showed precocious foraging, a reduction of the duration of foraging and a decrease in the longevity of infected bees compared with non-infected ones. However, the results indicate that although negative effects can be caused by N. ceranae in AHB, these were of a moderate magnitude compared with similar reports on EHB in temperate areas. Further research is necessary to evaluate the long-term effect of N. ceranae on AHBs in relation to colony dynamics to better understand the absence of significant colony losses associated with this pathogen in tropical and subtropical Mexico.
KeywordsNosema ceranae foraging behavior longevity Nosemosis Africanized bees
We thank Dr. Robert Paxton and PhD candidate Alice Séguret for constructive discussions and revision of the manuscript. We also thank Dr. Fernando Puerto Manzano for assistance in the molecular evaluations and Manuel Vazquez and Omar Sosa for assistance in laboratory and field observations.
All authors have contributed equally to the work.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Cantwell, G.E. (1970) Standard methods for counting nosema spores. American Bee Journal 110 (6), 222–223Google Scholar
- Dussaubat, C., Maisonnasse, A., Crauser, D., Beslay, D., Costagliola, G., Soubeyrand, S., Kretzchmar, A., Le Conte, Y. (2013) Flight behavior and pheromone changes associated to Nosema ceranae infection of honeybee workers (Apis mellifera) in field conditions. J Invertebr Pathol. 113:42–51CrossRefPubMedGoogle Scholar
- García-Millán, M., Quezada-Euán, J.J. (1993) Distribución de la Nosemosis en apiarios comerciales del estado de Yucatán. Apicultura Moderna 5, 22–24Google Scholar
- Guerrero-Molina, C., Correa-Benítez, A., Hamiduzzaman, M.M., Guzman-Novoa, E. (2016) Nosema ceranae is an old resident of honey bee (Apis mellifera) colonies in Mexico, causing infection levels of one million spores per bee or higher during summer and fall. J. Invertebr. Pathol. 141, 38–40CrossRefPubMedGoogle Scholar
- Higes, M., Martín-Hernández, R., Garrido-Bailón, E., González-Porto, A.V., García-Palencia, P., Meana, A., Del Nozal, M.J., Mayo, R., Bernal, J.L. (2009) Honeybee colony collapse due to Nosema ceranae in professional apiaries. Environ. Microbiol. Rep. 1, 110–113Google Scholar
- Lecocq, A., Jensen, A., Kryger. P., Nieh, J. (2016) Parasite infection accelerates age polyethism in Young honey bees. Sci. Rep., DOI: https://doi.org/10.1038/srep22042
- Martínez-Puc, J.F., Medina-Medina, L.A., Catzin-Ventura, G. (2011) Frecuencia de Varroa destructor, Nosema apis y Acarapis woodi en colonias manejadas y enjambres silvestres de abejas (Apis mellifera) en Mérida, Yucatán, México. Rev. Mex. Cien. Pecu. 2 (1), 25–38Google Scholar
- Nielsen D.I., Ebert, P.R., Hunt, G.R., Guzmán-Novoa, E., Kinnee, S.A., Page, R.E. (1999) Identification of Africanized honey bees (Hymenoptera: Apidae) incorporating morphometrics and an improved polymerase chain reaction mitotyping procedure. A. Entom. Soc. Ame. 92 (2), 167–174CrossRefGoogle Scholar
- Santos, L.G.; Alves, M.L., Message, D., Pinto, F.A., Silva, M.V., Teixeira, E.W. (2014) Honey bee health in apiaries in the Vale do Paraíba, São Paulo State, Southeastern Brazil. Sociobiology 6 (13), 307–312Google Scholar
- Seeley, T D. (1995) The Wisdom of the Hive. Harvard University Press, Cambridge, MA, USAGoogle Scholar
- SIAP-SAGARPA (2016) Abejas: población apícola 2006–2015, Colmenas [Online] https://www.gob.mx/cms/uploads/attachment/file/165992/abeja.pdf (accessed on 19 October 17)
- Therneau, T.M., Lumley, T. (2015). Package “survival”. R. Top. Doc., 128 Google Scholar