Changes in volatile organic compounds and differential expression of aroma-related genes during flowering of Rosa rugosa ‘Shanxian’

  • Yaru Sun
  • Wenli WangEmail author
  • Lanyong Zhao
  • Chengshu Zheng
  • Fangfang Ma
Review Article


Rosa rugosa ‘Shanxian’, a local variety of rose in Shandong Province, was studied for its aroma characteristics, including changes in aroma components and differential expression of aroma-related genes during flowering. The volatiles emitted from flowers were collected by headspace solid-phase microextraction and analyzed by gas chromatography–mass spectrometry at the budding stage, the early, half and full opening stages, and the withering stage. Sixty-six compounds were identified, and the full opening stage contained the highest level of every single aroma component compared with the other four stages. The major constituents of R. rugosa ‘Shanxian’ were alcohols, esters, ketones, aldehydes, phenols, and terpenes, with alcohols being the most abundant. The aroma of R. rugosa ‘Shanxian’ was mainly characterized by rich fruit aromas, floral aromas, and soft plant aromas. RrAAT, RrDXR, and RrDXS genes played a key role in monoterpene biosynthesis in R. rugosa ‘Shanxian’. The temporal and spatial expression of RrAAT, RrDXR, and RrDXS were measured at different flower developmental stages. Among the three genes, the overall expression level of RrAAT was significantly higher than that of RrDXR and RrDXS and was associated with the accumulation of acetate esters in R. rugosa, which could be used as an important candidate gene for rose metabolism. Taken together, our results demonstrated that R. rugosa ‘Shanxian’ has better aromatic characteristics than R. rugosa ‘Fenghua’. With a particularly prominent aroma that is strong and sweet, ‘Shanxian’ should be further investigated as a valuable breeding material that could be used to enhance genetic diversity and develop new rose varieties.


Rosa rugosa ‘Shanxian’ Florescence process Volatile component Aroma Gene expression 



We are grateful to Chao Wang for GC–MS analyzing, and Xiaotong Bao for sampling assistance (Shandong Agricultural University). We also thank Dr. Fangfang Ma (Shandong Agricultural University) for critically reading of the manuscript. This research was supported by the Natural Science Foundation of Shandong Province (ZR2011CM048) and the Agricultural Breeding Project of Shandong Province (Shandong Agricultural [2012]213).

Author's contribution

Wenli Wang is fully responsible for the research and revision of the manuscript. Wenli Wang and Yaru sun designed experiments and analyzed experimental results. Yarusun carried out experiments and wrote the manuscript. Lanyong Zhao assisted in the analysis of aroma component data and evaluation and instructed the revision of the corresponding contents of the manuscript. Chengshu Zheng gave guidance and participated in the discussion on functional gene expression analysis. Fangfang Ma contributed much in the data analysis and revised version of our manuscript.

Compliance with ethical standards

Conflict of interest

The manuscript have not been published in other refereed publications. Submission of a manuscript to the journal implies no concurrent submission elsewhere. The authors have no conflicting interests, and all authors have approved the manuscript and agree with its submission to the journal Horticulture, Environment, and Biotechnology (HEB). The publication of this manuscript has been approved by all co-authors.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

13580_2019_166_MOESM1_ESM.xlsx (2.6 mb)
Supplementary material 1 (XLSX 2676 kb)


  1. Chan WS, Abdullah JO, Namasivayam P, Mahmood M (2009) Molecular characterization of a new 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) transcript from Vanda Mimi Palmer. Sci Hortic 121:378–382. CrossRefGoogle Scholar
  2. Chen T, Li JJ, Chen JL, Song HP, Yang CH (2015) Anti-hyperplasia effects of Rosa rugosa polyphenols in rats with hyperplasia of mammary gland. Environ Toxicol Phar 39:990–996. CrossRefGoogle Scholar
  3. Compilation Committee of Shanxian County local chronicles of Shandong province (1996) Shanxian County Records. Shandong People’s Publishing House, Jin’an, China, pp 25–83Google Scholar
  4. Cordoba E, Salmi M, León P (2009) Unravelling the regulatory mechanisms that modulate the MEP pathway in higher plants. J Exp Bot 60:2933–2943. CrossRefGoogle Scholar
  5. Cordoba E, Porta H, Arroyo A, Román CS, Medina L, Rodríguez-Concepción M, León P (2011) Functional characterization of the three genes encoding 1-deoxy-d-xylulose 5-phosphate synthase in maize. J Exp Bot 62:2023–2038. CrossRefGoogle Scholar
  6. Dudareva N, Cseke L, Blanc VM, Pichersky E (1996) Evolution of floral scent in Clarkia: novel patterns of S-linalool synthase gene expression in the C. breweri flower. Plant Cell 8:1137–1148. Google Scholar
  7. Feng LG, Sheng LX, Zhao LY, Yu XY, Shao DW, He XD (2008) Changes of the aroma constituents and contents in the course of Rosa rugosa Thunb. flower development. Sci Agric Sin 41:4341–4351Google Scholar
  8. Feng LG, Chen C, Sheng LX, Liu P, Tao J, Su JL, Zhao LY (2010) Comparative analysis of headspace volatiles of Chinese Rosa rugosa. Molecules 15:8390–8399. CrossRefGoogle Scholar
  9. Feng LG, Chen C, Li TL, Wang M, Tao J, Zhao DQ, Sheng LX (2014) Flowery odor formation revealed by differential expression of monoterpene biosynthetic genes and monoterpene accumulation in rose (Rosa rugosa Thunb.). Plant Physiol Biochem 75:80–88. CrossRefGoogle Scholar
  10. Feng LG, Meng W, Jia W, Shu Z, Wei X, Sheng LX (2015) Isolation of 2-phenylethanol biosynthesis related genes and their relationship with 2-phenylethanol accumulation in Rosa rugosa. Acta Physiol Plant 37:256. CrossRefGoogle Scholar
  11. Forney CF, Kalt W, Jordan MA (2000) The composition of strawberry aroma is influenced by cultivar, maturity and storage. HortScience 35:1022–1026. CrossRefGoogle Scholar
  12. Hou XJ, Han JC, Yuan CS, Ren HH, Zhang Y, Zhang T, Xu LX, Zheng QS, Chen W (2016) Cardioprotective effects of total flavonoids extracted from Xinjiang Sprig Rosa rugosa against acute ischemia/reperfusion-induced myocardial injury in isolated rat heart. Cardiovasc Toxicol 16:54–66. CrossRefGoogle Scholar
  13. Kolosova N, Gorenstein N, Kish CM, Dudareva N (2001) Regulation of circadian methyl benzoate emission in diurnally and nocturnally emitting plants. Plant Cell 13:2333–2347. CrossRefGoogle Scholar
  14. Kurkcuoglu M, Baser KHC (2003) Studies on Turkish rose concrete, absolute, and hydrosol. Chem Nat Compd 39:457–464. CrossRefGoogle Scholar
  15. Li M, Tian YY, Geng J (2009) Analysis on peroxidase zymograms of shandong Rugosa Rose. Lishizhen Med Mater Med Res 20:1624–1625Google Scholar
  16. Lin XY (2008) Perfumery. Chemical Industry Publishing House, Beijing, pp 52–65Google Scholar
  17. Liu JZ, Liu JH, Dong FY, Cheng CG (1991) Analysis of Shanxian County rose oil. Shandong Sci 1:1–4Google Scholar
  18. Maciag A, Kalemba D (2015) Composition of rugosa rose (Rosa rugosa thunb.) hydrolate according to the time of distillation. Phytochem Lett 11:373–377. CrossRefGoogle Scholar
  19. Magnard JL, RocciaA Caissard JC, Vergne P, Sun P, Hecquet R, Dubois A, Oyant LH-S, Jullien F et al (2015) Biosynthesis of monoterpene scent compounds in roses. Science 349:81–83. CrossRefGoogle Scholar
  20. Ohloef G, Demole E (1987) Importance of the odoriferous principle of Bulgarian rose oil in flavour and fragrance chemistry. J Chromatogr 406:181–183. CrossRefGoogle Scholar
  21. Pichersky E, Lewinsohn E, Croteau R (1995) Purification and characterization of S-linalool synthase, an enzyme involved in the production of floral scent in Clarkia breweri. Arch Biochem Biophys 316:803–807. CrossRefGoogle Scholar
  22. Rodríguez-Concepción M, Ahumada I, Diez-Juez E, Sauret-Güeto S, Lois LM, Gallego F, Carretero-Paulet L, Campos N, Boronat A (2001) 1-Deoxy-d-xylulose 5-phosphate reductoisomerase and plastid isoprenoid biosynthesis during tomato fruit ripening. Plant J 27:213. CrossRefGoogle Scholar
  23. Shalit M, Guterman I, Volpin H, Bar E, Tamari T, Menda N, Adam Z, Zamir D, Vainstein A et al (2003) Volatile ester formation in roses: identification of an acetyl-coenzyme A. Geraniol/citronellol acetyltransferase in developing rose petals. Plant Physiol 131:1868–1876. CrossRefGoogle Scholar
  24. Singh U, Dwivedi P, Sangwan RS, Mishra BB (2017) In situ rose oxide enrichment led valorization of citronella (Cymbopogon winterianus) essential oil. Ind Crops Prod 97:567–573. CrossRefGoogle Scholar
  25. Sparinska A, Rostoks N (2012) Comparing ornamental and other quality traits of Rugosa hybrids in Latvia. Acta Hortic 953:277–283. CrossRefGoogle Scholar
  26. Sparinska A, Rostoks N (2015) Volatile organic compounds of hybrid rugosa roses in Latvia. Proc Latv Acad Sci 39:57–61. Google Scholar
  27. Spurrier S (1984) The Academic Du Vin Concise Guide to French Country Wines. Willow Books, PerigeeGoogle Scholar
  28. Svejda F (1977) Breeding for improvement of flowering attributes of winterhardy Rosa rugosa hybrids. Euphytica 26:697–701. CrossRefGoogle Scholar
  29. Tholl D, Gershenzon J (2015) The flowering of a new scent pathway in rose. Science 349:28–29. CrossRefGoogle Scholar
  30. Verma RS, Padalia RC, Chauhan A, Singh A, Yadav AK (2011) Volatile constituents of essential oil and rose water of damask rose (Rosa damascena Mill.) cultivars from North Indian hills. Nat Prod Res 25:1577–1584. CrossRefGoogle Scholar
  31. Wang XR (2008) Molecular cloning of DXR and DXS gene and study on metabolic pathway of terpene aroma in Hedychiumcoronarium. Master Dissertation, Guangdong. Agricultural University South China, pp 59–60Google Scholar
  32. Wilches I, Tobar V, Peñaherrera E, Cuzco N, Jerves L, Vander-HeydenY León-TamarizF, Vila E (2015) Evaluation of anti-inflammatory activity of the methanolic extract from Jungia rugosa leaves in rodents. J Ethnopharmacol 173:166–171. CrossRefGoogle Scholar
  33. Wu DM, Shao DW, Feng LG, Zhao LY (2013) Study on antioxidant capacity of dried flower buds of different rose cultivars. North Hortic 17:67–70Google Scholar
  34. Xu Y (2003) International Standard for Rose Oil (ISO9842:2003). Flavour Fragr Cosmet 6:36–37Google Scholar
  35. Yang LN, Ren JW, Wang Y (2014) Chemical investigation of volatiles emitted from flowers of three varieties of damask rose cultivated in Beijing. Hortic Environ Biotechnol 55:524–530. CrossRefGoogle Scholar
  36. Zhao CY, Xue J, Cai XD, Guo J, Li B, Wu S (2016) Assessment of the key aroma compounds in rose-based products. J Food Drug Anal 24:471–476. CrossRefGoogle Scholar
  37. Zheng ZX (2011) Research advances in 1-deoxy-d-xylulose-5-phosphate reductoisomerase, a key enzyme in plant isoprenoid biosynthesis. J Anhui Agric Sci 39:5695–5696Google Scholar

Copyright information

© Korean Society for Horticultural Science 2019

Authors and Affiliations

  1. 1.College of Horticulture Science and EngineeringShandong Agricultural UniversityTai’anChina
  2. 2.College of ForestryShandong Agricultural UniversityTai’anChina

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