Construction of genetic linkage map for Ficus carica L. based on AFLP, SSR, and SRAP markers
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A new genetic linkage map of Ficus carica (2n = 2x = 26) was constructed using 149 F1 progeny derived from the cross between two fig cultivars, ‘Bursa Siyahı’ (BS) and ‘Ak Ilek’ (AK). Fifty-two amplified fragment length polymorphism, 49 simple sequence repeat (SSR), 16 sequence-related amplified polymorphism (SRAP), and 12 sequence characterized amplified region (SCAR–SRAP) combinations were used to generate markers for the map. The BS map consisted of 229 markers, distributed to 16 linkage groups (LGs), with an average marker density of 5.98 cM and map distance of 1342 cM. The AK linkage map carried 244 markers, distributed to 16 LGs, with an average marker density of 4.90 cM and map distance of 1191 cM. The consensus map comprises 355 markers, 1474 cM in length, with average marker density of 4.15 cM. The map indicates the location of new SSRs, nine of which were transferred from related species, and might be helpful for mapping quantitative trait loci that control important horticultural traits in the future.
KeywordsFig Molecular markers F1 pseudo-test cross Consensus map ‘Bursa Siyahı’ ‘Ak Ilek’
We greatly thank and acknowledge to Scientific and Technological Research Council of Turkey (TUBITAK) for financial support of this study (Project No: TUBITAK-TOVAG-110O659), to Erbeyli Fig Research Institute-Aydin for their contribution and Akdeniz University for laboratory infrastructure and facilities. We also thank to Hilmi Kocatas for F1 progeny plants and Inci Sahin for technical help.
HI, CI, OG, NM, designed the research; HI, SSC performed the research; HI, CI analyzed the data; HI wrote the manuscript; and CI, OG and NM edit the manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Balas FC, Osuna MD, Domínguez G, Pérez-Gragera F, López-Corrales M (2014) Ex situ conservation of underutilised fruit tree species: establishment of a core collection for Ficus carica L. using microsatellite markers (SSRs). Tree Genet Genomes. https://doi.org/10.1007/s11295-014-0715-3 Google Scholar
- Blenda AV, Verde I, Georgi LL, Reighard GL, Forrest SD, Muñoz-Torres M, Baird WV, Abbott AG (2007) Construction of a genetic linkage map and identification of molecular markers in peach rootstocks for response to peach tree short life syndrome. Tree Genet Genomes 3:341–350. https://doi.org/10.1007/s11295-006-0074-9 CrossRefGoogle Scholar
- De Candolle A (1886) Origin of cultivated plants (reprint of 2nd edition, 1967). Hafner Publishing, New YorkGoogle Scholar
- Di Pierro EA, Gianfranceschi L, Di Guardo M, Koehorst-Van Putten HJ, Kruisselbrink JW, Longhi S, Troggio M, Bianco L, Muranty H, Pagliarani G et al (2016) A high-density, multi-parental SNP genetic map on apple validates a new mapping approach for outcrossing species. Hortic Res. https://doi.org/10.1038/hortres.2016.57 Google Scholar
- Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:115Google Scholar
- FAOSTAT (2012) Food and Agriculture Organization of the United Nation Statistics for 2012. http://www.fao.org/faostat/en/#rankings/countries_by_commodity. Accessed 14 Nov 2018
- Grattapaglia D, Sederoff R (1994) Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics 137:1121–1137Google Scholar
- Maliepaard C, Alston FH, Van Arkel G, Brown LM, Chevreau E, Dunemann F, Evans KM, Gardiner S, Guilford P, Van Heusden AW et al (1998) Aligning male and female linkage maps of apple (Malus pumila Mill.) using multi-allelic markers. Theor Appl Genet. https://doi.org/10.1007/s001220050867 Google Scholar
- Mori K, Shirasawa K, Nogata H, Hirata C, Tashiro K, Habu T, Kim S, Himeno S, Kuhara S, Ikegami H (2017) Identification of RAN1 orthologue associated with sex determination through whole genome sequencing analysis in fig (Ficus carica L.). Sci Rep 7:1–12. https://doi.org/10.1038/srep41124 CrossRefGoogle Scholar
- Olmstead JW, Sebolt AM, Cabrera A, Sooriyapathirana SS, Hammar S, Iriarte G, Wang D, Chen CY, Van Der Knaap E, Iezzoni AF (2008) Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map. Tree Genet Genomes. https://doi.org/10.1007/s11295-008-0161-1 Google Scholar
- Parrish TL (2003) in Krakatau: genetic consequences of island colonization, Chapter 5. Identification of a male-specific AFLP marker in a functionally dioecious fig Ficus fulva Reinw. Ex. Bl. (Moraceae). Universiteit Utrecht, Netherland, PhD Diss.Google Scholar
- Schouten HJ, van de Weg WE, Carling J, Khan SA, McKay SJ, van Kaauwen MPW, Wittenberg AHJ, Koehorst-van Putten HJJ, Noordijk Y, Gao Z et al (2012) Diversity arrays technology (DArT) markers in apple for genetic linkage maps. Mol Breed 29:645–660. https://doi.org/10.1007/s11032-011-9579-5 CrossRefGoogle Scholar
- Storey WB (1975) Figs. In: Janick J, Moore JN (eds) Advances in fruit breeding. Purdue Univ. Press, West Lafayette, pp 568–589Google Scholar
- Venkateswarlu M, Urs SR, Nath BS, Shashidhar HE, Maheswaran M, Veeraiah TM, Sabitha MG (2006) A first genetic linkage map of mulberry (Morus spp.) using RAPD, ISSR, and SSR markers and pseudotestcross mapping strategy. Tree Genet Genomes 3:15–24. https://doi.org/10.1007/s11295-006-0048-y CrossRefGoogle Scholar
- Vignes H, Hossaert-Mckey M, Beaune D, Fevre D, Anstett MC, Borges RM, Kjellberg F, Chevallier MH (2006) Development and characterization of microsatellite markers for a monoecious Ficus species, Ficus insipida, and cross-species amplification among different sections of Ficus. Mol Ecol Notes. https://doi.org/10.1111/j.1471-8286.2006.01347.x Google Scholar