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Interspecies hybrids of Panax ginseng Meyer new line 0837 and Panax quinquefolius generated superior F1 hybrids with greater biomass and ginsenoside contents

  • Kwang Hoon Seo
  • Padmanaban Mohanan
  • Dong-Uk Yang
  • Yu-Jin Kim
  • Woo-Saeng Kwon
  • Deok Chun YangEmail author
Research Report
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Abstract

Panax ginseng and Panax quinquefolius are important herbs with similar benefits and medicinal properties. Both possess ginsenosides, the pharmacologically active components, abundantly in their roots. In an attempt to establish ginseng cultivars with improved agronomical characters and higher ginsenoside content, reciprocal interspecific hybrids were generated by artificial pollination of emasculated flowers. Three elite cultivars of P. ginseng, types Jakyung, Chungkyung, and Hwangsook, and two new lines, 0837 and 0702, were used to assess interspecific hybrid potential. Among F1 reciprocal hybrids generated using line 0837 as either the maternal or paternal parent showed excellent agronomical traits and hybrid vigor. They showed strong root phenotypes with many lateral and fine roots resulting in 10 to 20% higher ginsenoside content compared to the parental lines. Among the major ginsenosides, pharmacologically active Re, Rb1, Rb2, and F1 were enriched. They accounted for 70% of the protopanaxadiol ginsenosides in 0837/Pq and Pq/0837 crosses, displaying strong maternal dominance. Thus, based on our results, we conclude that P. ginseng line 0837 is superior compared to already existing lines for performing interspecific hybridization with promising outcomes in root quality and ginsenoside content.

Keywords

American ginseng Artificial pollination Ginsenosides Interspecific hybrids Korean ginseng Medicinal plants Perennial breeding 

Notes

Acknowledgement

This research was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (iPET, 112142-05-1-CG000), Republic of Korea (WS Kwon and DC Yang).

Author contributions

KW Seo and P Mohanan performed the experiments and analyze the data together with DU Yang. DC Yang, WS Kwon, YJ Kim designed the study and cultivar identification and overviewed the experiments. KW Seo and P Mohanan wrote the manuscript together with DC Yang.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13580_2019_154_MOESM1_ESM.pptx (56 kb)
Figure S1: A schematic representation of the development of 0702 and 0837 new line selection and interspecific cross time points. (PPTX 56 kb)
13580_2019_154_MOESM2_ESM.docx (22 kb)
Table S1: Phenotypic observation of 0837, Pq, 0837/Pq, and Pq/0837 populations from the first to fifth-year of development (DOCX 23 kb)

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Copyright information

© Korean Society for Horticultural Science 2019

Authors and Affiliations

  1. 1.Department of Oriental Medicinal Biotechnology, College of Life ScienceKyung Hee UniversityYongin siRepublic of Korea
  2. 2.Graduate School of Biotechnology, College of Life SciencesKyung Hee UniversityYongin siRepublic of Korea

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