Advertisement

Lysophosphatidylethanolamine delays fruit softening of persimmon (Diospyros kaki)

  • Jihye Jung
  • Young-Pyo Lee
  • Sung-Woo Bae
  • Gwang-Hwan Ahn
  • Stephen Beungtae RyuEmail author
Research Report
  • 1 Downloads

Abstract

We investigated the possibility of prolonging the freshness of persimmons (Diospyros kaki) for overseas export. Previous studies aimed at improving the shelf life of harvested persimmons focused on the synthetic gas 1-MCP, an inhibitor of ethylene perception. In this study, the natural lipid compound lysophosphatidylethanolamine (LPE) was applied to prolong the freshness of persimmons. LPE significantly delayed persimmon softening when applied not only during fruit growth, but also after harvest, or after a long period of post-harvest cold storage, simulating conditions of overseas export. LPE suppressed the expression of the softening-related genes ACS1, ACO1, ERF24, DkUGD1, DkCHI3, and DkB-CHI1. These results reveal the effects of LPE on persimmon softening and the related molecular mechanisms and provide evidence that LPE can be used to delay persimmon softening.

Keywords

Persimmon Diospyros kaki Fruit softening Lysophosphatidylethanolamine Ethylene biosynthesis genes Starch degradation genes 

Notes

Acknowledgements

This study was supported by grants from the Next-Generation BioGreen 21 Program (Project Nos. PJ01348601 & PJ01368201), the Rural Development Administration, Republic of Korea, and in part from the Korea Institute of Planning & Evaluation for Technology of Agriculture, Forestry, Fisheries and Food (IPET 112150-3), and the KRIBB Project Program.

Compliance with ethical standards

Conflict of interest

The authors have no conflict of interest to declare.

References

  1. Ahmed ZF, Palta JP (2015) A postharvest dip treatment with lysophosphatidylethanolamine, a natural phospholipid, may retard senescence and improve the shelf life of banana fruit. HortScience 50:1035–1040CrossRefGoogle Scholar
  2. Ahmed S, Thompson A, Perviez M, Ullah H, Chatha Z (2006) Effect of polyethylene film thickness and exposure time of ethylene on the ripening behavior and quality of banana. Int J Agric Biol 8:381–386Google Scholar
  3. Ahmed M, Yousef AR, Sarrwy S (2011) Modified atmosphere packaging for maintaining quality and shelf life extension of persimmon fruits. Asian J Agric Sci 3:308–316Google Scholar
  4. Alexander L, Grierson D (2002) Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening. J Exp Bot 53:2039–2055.  https://doi.org/10.1093/jxb/erf072 CrossRefGoogle Scholar
  5. Bhushan S, Tripathi S, Thakur N (2002) Effect of different modified atmosphere packaging on the quality of kiwifruit stored at room temperature. J Food Sci Technol 39:279–283Google Scholar
  6. Butt MS, Sultan MT, Aziz M, Naz A, Ahmed W, Kumar N, Imran M (2015) Persimmon (Diospyros kaki) fruit: hidden phytochemicals and health claims. EXCLI J 14:542.  https://doi.org/10.17179/excli2015-159 Google Scholar
  7. Çandir E, Özdemir AE, Kaplankiran M, Demirkeser TH, Yildiz E (2010) Storage life of non-astringent persimmons grown in the eastern Mediterranean. N Z J Crop Hortic Sci 38:1–6.  https://doi.org/10.1080/01140671003619266 CrossRefGoogle Scholar
  8. Choi H-S, Jung S-K, Kim Y-K (2013) Storage ability of non-astringent ‘Fuyu’ persimmon fruit is affected by various concentrations of 1-methylcyclopropene and/or modified atmosphere packaging. J Hortic Sci Biotechnol 88:195–200.  https://doi.org/10.1080/14620316.2013.11512956 CrossRefGoogle Scholar
  9. Cutillas-Iturralde A, Zarra I, Fry SC, Lorences EP (1994) Implication of persimmon fruit hemicellulose metabolism in the softening process. Importance of xyloglucan endotransglycosylase. Physiol Plant 91:169–176.  https://doi.org/10.1111/j.1399-3054.1994.tb00415.x CrossRefGoogle Scholar
  10. Dal Cin V, Rizzini FM, Botton A, Tonutti P (2006) The ethylene biosynthetic and signal transduction pathways are differently affected by 1-MCP in apple and peach fruit. Postharvest Biol Technol 42:125–133.  https://doi.org/10.1016/j.postharvbio.2006.06.008 CrossRefGoogle Scholar
  11. Fan L, Zheng S, Wang X (1997) Antisense suppression of phospholipase D alpha retards abscisic acid-and ethylene-promoted senescence of postharvest Arabidopsis leaves. Plant Cell 9:2183–2196.  https://doi.org/10.1105/tpc.9.12.2183 Google Scholar
  12. Farag KM, Palta JP (1991) Improving postharvest keeping quality of vine-ripened tomato fruits with a natural lipid. HortScience 26:778Google Scholar
  13. Farag KM, Palta JP (1993) Use of lysophosphatidylethanolamine, a natural lipid, to retard tomato leaf and fruit senescence. Physiol Plant 87:515–521.  https://doi.org/10.1111/j.1399-3054.1993.tb02501.x CrossRefGoogle Scholar
  14. Gross KC, Wallner SJ (1979) Degradation of cell wall polysaccharides during tomato fruit ripening. Plant Physiol 63:117–120.  https://doi.org/10.1104/pp.63.1.117 CrossRefGoogle Scholar
  15. Harima S, Nakano R, Yamauchi S, Kitano Y, Kubo Y, Inaba A, Tomita E (2002) Inhibition of fruit softening in forcing-cultured ‘Tonewase’ Japanese persimmon by packaging in perforated and non-perforated polyethylene bags. J Jpn Soc Hortic Sci 71:284–291.  https://doi.org/10.2503/jjshs.71.284 CrossRefGoogle Scholar
  16. Harima S, Nakano R, Yamauchi S, Kitano Y, Yamamoto Y, Inaba A, Kubo Y (2003) Extending shelf-life of astringent persimmon (Diospyros kaki Thunb.) fruit by 1-MCP. Postharvest Biol Technol 29:319–324.  https://doi.org/10.1016/S0925-5214(03)00058-9 CrossRefGoogle Scholar
  17. Hong JH (2012) Lysophosphatidylethanolamine treatment delays leaf senescence and improve fruit storability in melon (Cucumis melo L.). Korean J Hortic Sci Technol 30:158–161.  https://doi.org/10.7235/hort.2012.12046 CrossRefGoogle Scholar
  18. Itamura H, Ohno Y, Yamamura H (1997) Characteristics of fruit softening in Japanese persimmon ‘Saijo’. International Society for Horticultural Science (ISHS), Leuven, pp 179–188Google Scholar
  19. Jiang Y, Joyce D (2000) Effects of 1-methylcyclopropene alone and in combination with polyethylene bags on the postharvest life of mango fruit. Ann Appl Biol 137:321–327.  https://doi.org/10.1111/j.1744-7348.2000.tb00073.x CrossRefGoogle Scholar
  20. Jiang Y, Joyce DC, Macnish AJ (1999) Extension of the shelf life of banana fruit by 1-methylcyclopropene in combination with polyethylene bags. Postharvest Biol Technol 16:187–193.  https://doi.org/10.1016/S0925-5214(99)00009-5 CrossRefGoogle Scholar
  21. Jiang N, Rao J, Fu R, Suo J (2010) Effects of propylene and 1-methylcyclopropene on PG activities and expression of DkPG1 gene during persimmon softening process. Acta Hortic Sin 37:1507–1512Google Scholar
  22. Jung S-K, Choi H-S (2015) Effects of 1-MCP or packaging film on the quality and gas composition of ‘Fuji’ apple fruit during long-term storage. J Hortic Sci Biotechnol 90:344–348.  https://doi.org/10.1080/14620316.2015.11513193 CrossRefGoogle Scholar
  23. Jung J, Choi SC, Jung S, Cho B-K, Ahn G-H, Ryu SB (2017) A transcriptome approach toward understanding fruit softening in persimmon. Front Plant Sci 8:1556.  https://doi.org/10.3389/fpls.2017.01556 CrossRefGoogle Scholar
  24. Kaur N, Palta JP (1997) Postharvest dip in a natural lipid, lysophosphatidylethanolamine, may prolong vase life of snapdragon flowers. HortScience 32:888–890CrossRefGoogle Scholar
  25. Kim Y-K, Lee J-M (2004) Extension of storage and shelf-life of sweet persimmon with 1-MCP. In: III International Symposium on Persimmon, vol 685, pp 165–175Google Scholar
  26. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408.  https://doi.org/10.1006/meth.2001.1262 CrossRefGoogle Scholar
  27. Lownds N, Banaras M, Bosland P (1994) Postharvest water loss and storage quality of nine pepper (Capsicum) cultivars. HortScience 29:191–193CrossRefGoogle Scholar
  28. Nakano R, Harima S, Ogura E, Inoue S, Kubo Y, Inaba A (2001) Involvement of stress-induced ethylene biosynthesis in fruit softening of ‘Saijo’ persimmon. J Jpn Soc Hortic Sci 70:581–585.  https://doi.org/10.2503/jjshs.70.581 CrossRefGoogle Scholar
  29. Nakano R, Ogura E, Kubo Y, Inaba A (2003) Ethylene biosynthesis in detached young persimmon fruit is initiated in calyx and modulated by water loss from the fruit. Plant Physiol 131:276–286.  https://doi.org/10.1104/pp.010462 CrossRefGoogle Scholar
  30. Nishiyama K, Guis M, Rose JK, Kubo Y, Bennett KA, Wangjin L, Kato K, Ushijima K, Nakano R, Inaba A (2007) Ethylene regulation of fruit softening and cell wall disassembly in Charentais melon. J Exp Bot 58:1281–1290.  https://doi.org/10.1093/jxb/erl283 CrossRefGoogle Scholar
  31. Özgen M, Farag KM, Ozgen S, Palta JP (2005) Lysophosphatidylethanolamine accelerates color development and promotes shelf life of cranberries. HortScience 40:127–130CrossRefGoogle Scholar
  32. Özgen M, Serçe S, Akça Y, Hong JH (2015) Lysophosphatidylethanolamine (LPE) improves fruit size, color, quality and phytochemical contents of Sweet Cherry cv ‘0900 Ziraat’. Korean J Hortic Sci Technol 33:196–201.  https://doi.org/10.7235/hort.2015.13195 CrossRefGoogle Scholar
  33. Paniagua A, East A, Hindmarsh J, Heyes J (2013) Moisture loss is the major cause of firmness change during postharvest storage of blueberry. Postharvest Biol Technol 79:13–19.  https://doi.org/10.1016/j.postharvbio.2012.12.016 CrossRefGoogle Scholar
  34. Ryu SB, Wang X (1995) Expression of phospholipase D during castor bean leaf senescence. Plant Physiol 108:713–719.  https://doi.org/10.1104/pp.108.2.713 CrossRefGoogle Scholar
  35. Ryu SB, Karlsson BH, Özgen M, Palta JP (1997) Inhibition of phospholipase D by lysophosphatidylethanolamine, a lipid-derived senescence retardant. Proc Natl Acad Sci USA 94:12717–12721.  https://doi.org/10.1073/pnas.94.23.12717 CrossRefGoogle Scholar
  36. Salvador A, Cuquerella J, Martínez-Jávega J, Monterde A, Navarro P (2004) 1-MCP preserves the firmness of stored persimmon ‘Rojo Brillante’. J Food Sci 69:69–73.  https://doi.org/10.1111/j.1365-2621.2004.tb15516.x Google Scholar
  37. Sitrit Y, Bennett AB (1998) Regulation of tomato fruit polygalacturonase mRNA accumulation by ethylene: a re-examination. Plant Physiol 116:1145–1150.  https://doi.org/10.1104/pp.116.3.1145 CrossRefGoogle Scholar
  38. Sivertsvik M, Rosnes JT, Bergslien H (2002) Modified atmosphere packaging. In: Minimal processing technologies in the food industry. CRC, New York, pp 61–86Google Scholar
  39. Tata SK, Jung JH, Kim YH, Choi JY, Jung JY, Lee IJ, Shin JS, Ryu SB (2016) Heterologous expression of chloroplast-localized geranylgeranyl pyrophosphate synthase confers fast plant growth, early flowering and increased seed yield. Plant Biotechnol J 14:29–39.  https://doi.org/10.1111/pbi.12333 CrossRefGoogle Scholar
  40. Vilas-Boas EVDB, Kader AA (2007) Effect of 1-methylcyclopropene (1-MCP) on softening of fresh-cut kiwifruit, mango and persimmon slices. Postharvest Biol Technol 43:238–244.  https://doi.org/10.1016/j.postharvbio.2006.09.010 CrossRefGoogle Scholar
  41. Wan Zaliha WS, Singh Z (2012) Lysophosphatidylethanolamine improves fruit colour and accumulation of anthocyanin in ‘cripps pink’ apples. International Society for Horticultural Science (ISHS), Leuven, pp 227–232Google Scholar
  42. Wang X (2005) Regulatory functions of phospholipase D and phosphatidic acid in plant growth, development, and stress responses. Plant Physiol 139:566–573.  https://doi.org/10.1104/pp.105.068809 CrossRefGoogle Scholar
  43. Watkins CB (2006) The use of 1-methylcyclopropene (1-MCP) on fruits and vegetables. Biotechnol Adv 24:389–409.  https://doi.org/10.1016/j.biotechadv.2006.01.005 CrossRefGoogle Scholar
  44. Win NM, Lee J, Yoo J, Ryu S, Kim K-O, Kim DH, Jung HY, Choung M-G, Park K-I, Cho Y-J, Kang I-K (2018) Effects of polyethylene film liner, 1-methylcyclopropene, and aminoethoxyvinylglycine treatments on fruit quality attributes of ‘tonewase’ persimmon fruits during cold storage. Hortic Sci Technol 36:256–265.  https://doi.org/10.12972/kjhst.20180026 Google Scholar
  45. Zhong S, Fei Z, Chen Y-R, Zheng Y, Huang M, Vrebalov J, Mcquinn R, Gapper N, Liu B, Xiang J (2013) Single-base resolution methylomes of tomato fruit development reveal epigenome modifications associated with ripening. Nat Biotechnol 31:154.  https://doi.org/10.1038/nbt.2462 CrossRefGoogle Scholar

Copyright information

© Korean Society for Horticultural Science 2019

Authors and Affiliations

  1. 1.Plant Systems Engineering Research CenterKorea Research Institute of Bioscience and Biotechnology (KRIBB)DaejeonKorea
  2. 2.Department of Biological SciencesKorea Advanced Institute of Science and Technology (KAIST)DaejeonKorea
  3. 3.Department of Biosystems and Bioengineering, KRIBB School of BiotechnologyUniversity of Science and Technology (UST)DaejeonKorea
  4. 4.Sweet Persimmon Research InstituteGimhaeKorea

Personalised recommendations