New molecular record and some biochemical features of the rare plant species of Iranian lily (Lilium ledebourii Boiss.)
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The antioxidative potential and some phytochemicals of Lilium ledebourii Boiss. were biochemically analyzed using assays of extracts from the bulb, shoot and flower. In addition, the plant’s biosystematics was molecularly characterized using a phylogenetic tree. L. ledebourii plants were collected from their natural habitat. Biochemical assays showed a higher total phenolic and total flavonoid content in the leaf than in the bulb (by 264.9% and 27.38%, respectively) and flower (by 331.18% and 47.85%, respectively). The flower had the highest total anthocyanin of all the organs. The DPPH-based antioxidant activity assay showed the highest activity level in the bulb, whereas FRAP- and BCLS-based assays revealed higher antioxidant activity in the leaf than in the bulb (by 7.1 and 3 folds, respectively) and flower (by 4.9 and 1.5 folds, respectively). Our results showed that phenolic and flavonoid compositions were positively associated with antioxidative potential as assayed by FRAP and BCLS methods. The 5.8S ribosomal DNA sequence was extracted by PCR from genomic DNA and sequenced. Our molecular study resulted in a sequencing record of L. ledebourii var. Kelardasht Salehi 1 in Genbank with the accession number KX495217.1. We conclude that the antioxidant potential of L. ledebourii is strongly dependent on the organ and assay method.
KeywordsAntioxidative potential Biosystematics Lilium ledebourii 5.8S ribosomal DNA
This work was supported by a grant from research council of University of Guilan and University of Zanjan. We thank to Dr. Ramin Abdoli and Dr. Abbas Jorkesh for their help.
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