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Optimal conditions for spore germination and gametophyte and sporophyte production in the autumn fern Dryopteris erythrosora

  • Bo Kook Jang
  • Ju Sung Cho
  • Hyuk Joon Kwon
  • Cheol Hee Lee
Research Report
  • 41 Downloads

Abstract

Dryopteris erythrosora (D. C. Eaton) Kuntze is used as an ornamental horticultural crop and landscaping plant, and it is a plant with high development value as an herbal medicine, but there are few studies to proliferate a large amount of plants. This study aimed to determine a suitable method for spore germination, gametophyte proliferation, sporophyte formation, and seedling production in D. erythrosora. Spore germination rate was highest in 1/2 × Murashige and Skoog (MS) medium (92.8%). A suitable medium for prothallus proliferation was selected based on gametophytes produced from germinated spores. A 1 × MS medium was most effective for gametophyte proliferation and active organ formation; moreover, 1 × MS medium containing 1% sucrose without activated charcoal was the most effective growth condition for inducing gametophyte growth and development. The suitable culture soil composition for sporophyte formation was investigated by varying the ratio of horticultural substrate, peat moss, perlite, and decomposed granite. We found that a 2:1 (v:v) mixture of horticultural substrate and perlite accelerated sporophyte formation (155 units). Furthermore, suitable seedlings and transplants for sporophyte seedling growth were determined by varying the ratio of horticultural substrate and decomposed granite, as well as plug tray cell size. For sporophyte seedlings, the greatest growth in root length (92.9 mm) and the most aerial and underground fresh weight (234.1 and 40.3 g, respectively) were observed in a mixture of horticultural substrate and decomposed granite at a 1:2 (v:v) ratio. Our results using tissue culture technology will be provided as a mass production method for substantial industrialization of D. erythrosora plants.

Keywords

Dryopteridaceae Fern protonema Gametophyte chopping method Gametophyte grinding method Reddish-colored pinnae 

Notes

Acknowledgements

We are grateful to the Department of Horticultural Science, Chungbuk National University, and Brain Korea 21 Center for Bio-Resource Development for their support of and contributions to this work.

Supplementary material

13580_2018_97_MOESM1_ESM.pdf (143 kb)
Supplementary material 1 (PDF 143 kb)

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Copyright information

© Korean Society for Horticultural Science 2018

Authors and Affiliations

  1. 1.Brain Korea 21 Center for Bio-Resource Development, Division of Animal, Horticultural, and Food SciencesChungbuk National UniversityCheongjuKorea
  2. 2.Quality Control and Seed Tech Team, Quality Assurance DepartmentNongwoo Bio CO., LTDYeojuKorea
  3. 3.National Institute of Biological ResourcesIncheonKorea

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