Horticulture, Environment, and Biotechnology

, Volume 59, Issue 4, pp 575–582 | Cite as

Callus induction and plant regeneration from leaves of peony

  • Xiangtao Zhu
  • Xueqin Li
  • Wenjie Ding
  • Songheng JinEmail author
  • Yan Wang
Research Report Tissue Culture/Biotechnology


Tree peony (Paeonia suffruticosa Andr.) is a valued ornamental plant. This study reports on peony callus induction, shoot organogenesis and plant regeneration using young peony leaves as explants. Various media containing diverse plant growth regulators were assessed for their potency in peony propagation. After exposure of dark-adapted leaf discs to 30 μmol m−2 s−1 of light, inoculation in Murashige and Skoog (MS) + 0.2 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) + 0.2 mg L−1 a-naphthaleneacetic acid (NAA) + 3.0 mg L−1 thidiazuron (TDZ) medium resulted to the highest callus induction rate with values reaching up to 87.8%. We identified that MS + 0.2 mg L−1 NAA + 2.0 mg L−1 6-benzyladenine (6-BA) + 2.0 mg L−1 kinetin (KT), with a multiplication coefficient of 3.025, to be the optimal medium for further callus proliferation under light. Inoculation in MS + 2.0 mg L−16-BA + 0.2 mg L−1 NAA + 0.3 mg L−1 TDZ medium allowed 22.22% of callus cultures to differentiate into adventitious shoots, whereas a similar rate of root formation was detected when 1/2 MS + 0.1 mg L−1 NAA + 0.05 mg L−1 IBA + 30 g L−1 sucrose medium was used. Our findings provide important information on peony regeneration and present a new method for peony tissue culture that will potentially facilitate mass propagation and genetic engineering of peony plants.


Paeonia suffruticosa Callus differentiation Plant growth regulators Root formation Morphological analysis 





2,4-Dichlorophenoxyacetic acid


a-Naphthaleneacetic acid






Plant growth regulators


Murashige and Skoog


Woody plant medium


Indole-3-butyric acid


Relative humidity



The work was partly supported by the Zhejiang Provincial Natural Science Foundation of China (LY16C160011), and the National Natural Science Foundation of China (Nos. 31170584 and 31200525).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Xiangtao Zhu
    • 1
  • Xueqin Li
    • 1
  • Wenjie Ding
    • 1
  • Songheng Jin
    • 1
    Email author
  • Yan Wang
    • 2
  1. 1.Jiyang CollegeZhejiang A & F UniversityZhuji ZhejiangPeople’s Republic of China
  2. 2.Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration Research Institute of ForestryChinese Academy of ForestryBeijingPeople’s Republic of China

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