Callus induction and plant regeneration from leaves of peony
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Tree peony (Paeonia suffruticosa Andr.) is a valued ornamental plant. This study reports on peony callus induction, shoot organogenesis and plant regeneration using young peony leaves as explants. Various media containing diverse plant growth regulators were assessed for their potency in peony propagation. After exposure of dark-adapted leaf discs to 30 μmol m−2 s−1 of light, inoculation in Murashige and Skoog (MS) + 0.2 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D) + 0.2 mg L−1 a-naphthaleneacetic acid (NAA) + 3.0 mg L−1 thidiazuron (TDZ) medium resulted to the highest callus induction rate with values reaching up to 87.8%. We identified that MS + 0.2 mg L−1 NAA + 2.0 mg L−1 6-benzyladenine (6-BA) + 2.0 mg L−1 kinetin (KT), with a multiplication coefficient of 3.025, to be the optimal medium for further callus proliferation under light. Inoculation in MS + 2.0 mg L−16-BA + 0.2 mg L−1 NAA + 0.3 mg L−1 TDZ medium allowed 22.22% of callus cultures to differentiate into adventitious shoots, whereas a similar rate of root formation was detected when 1/2 MS + 0.1 mg L−1 NAA + 0.05 mg L−1 IBA + 30 g L−1 sucrose medium was used. Our findings provide important information on peony regeneration and present a new method for peony tissue culture that will potentially facilitate mass propagation and genetic engineering of peony plants.
KeywordsPaeonia suffruticosa Callus differentiation Plant growth regulators Root formation Morphological analysis
Plant growth regulators
Murashige and Skoog
Woody plant medium
The work was partly supported by the Zhejiang Provincial Natural Science Foundation of China (LY16C160011), and the National Natural Science Foundation of China (Nos. 31170584 and 31200525).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Chen FY, Li JJ (1998) Exportation of Chinese tree peonies (Mudan) and their development in other countries: cultivated. J Northwest Normal Univ 34:109–116Google Scholar
- Gabryszewska E (1998) The influence of cytokinins, thidiazuron, paclobutrazoland red light on shoot proliferation of herbaceous peony cv. Jadwiga in vitro. J Fruit Ornam Plant Res 6:157–169Google Scholar
- Li YM, Luo XF (2004) Research development of in vitro breeding and fast propagation of Paeonia spp. J Southwest For Coll 24:70–73Google Scholar
- Li ZX, Qin GW, He JH, Cao XY (2010) Comparative analysis of fatty acid composition in seed kernel and coat of Paeonia rockii seeds. Seed 29:34–36Google Scholar
- Meyer MM (1976) Culture of Paeonia embryos by in vitro techniques. Am Peony Soc Bull 217:32–35Google Scholar
- Qin KJ, Li JJ (2000) Herbaceous peonies. Shanghai Scientific & Technical Publishers, ShanghaiGoogle Scholar
- Shannon J, Kamp JR (1959) Trials of various possible propagation methods on herbaceous peonies. State Flor Assoc Bull 197:4–7Google Scholar
- Wang HY, He SL, Tanaka M, Van PT, Silva JATD (2010a) Effects of 2,4-D on callus formation in tree peony (Paeonia suffruticosa) under different light conditions and light quality. Floric Ornam Biotechnol 4:99–102Google Scholar
- Wang JF, Li Q, Meng H (2010b) Induction and regeneration of callus tissues in five peony cultivars. J Beijing For Univ 32:213–216Google Scholar
- Wang HY, He SL, Tanaka M, Van PT, Silva JATD (2012) Effect of IBA concentration, carbon source, substrate, and light source on root induction ability of tree peony (Paeonia suffruticosa Andr.) plantlets in vitro. Eur J Hortic Sci 77:122–128Google Scholar
- Zhu XT, Wang Y, Wu Q, Zhang JL, Zhu KY (2015) Efficient induction of callus and plant regeneration from Paeonia suffruticosa Andr. J Nuclear Agric Sci 29:56–62Google Scholar