Horticulture, Environment, and Biotechnology

, Volume 59, Issue 2, pp 179–188 | Cite as

The effect of light quality on growth and endopolyploidy occurrence of in vitro-grown Phalaenopsis ‘Spring Dancer’

  • A-Reum Kwon
  • Myung-Min Oh
  • Kee-Yoeup Paek
  • So-Young Park
Research Report Cultivation Physiology
  • 59 Downloads

Abstract

In the present study, the effect of light quality on endoreduplication and growth in Phalaenopsis ‘Spring Dancer’ plantlets was studied. The response of protocorm-like body (PLB)-derived plantlets subjected to monochromatic red (R60), blue (B60), and various combinations of both lights was investigated. Flow cytometry was used to investigate the effect of light on endocycle and growth, cell division, and endopolyploidy levels. In addition, the activities of stress-related enzymes such as catalase (CAT) and peroxidase (POD) were analyzed from leaves and roots. After 8 weeks, the leaf area of plants grown under monochromatic R60 and B60 light was found to be higher than that of plants grown under other wavelengths of light, except the control plants (fluorescent light). These results revealed monochrome blue (B60) light increased the ratio of endoreduplicated cells (4C–8C). CAT activity was highest in leaves grown under R60; however, the oxidized phenol concentration in the culture medium was lowest under R60 while it was the highest under B60 and fluorescent light (F). This indicates that plantlets were less stressed under R60 than B60 or F. The results of this study reveal that stress induced by monochromatic light stimulates endopolyploidy in leaves, which may subsequently increase Phalaenopsis leaf size.

Keywords

Phalaenopsis Monochrome blue Monochrome red Endopolyploidy 

Notes

Acknowledgements

This work was supported by Brain Korea (BK) plus 21 project.

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Copyright information

© Korean Society for Horticultural Science and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • A-Reum Kwon
    • 2
  • Myung-Min Oh
    • 1
  • Kee-Yoeup Paek
    • 1
  • So-Young Park
    • 1
    • 2
  1. 1.Department of Horticultural Science, Brain Korea 21 Center for Bio-Resource DevelopmentChungbuk National UniversityCheongjuRepublic of Korea
  2. 2.WellGreen Co.CheongjuRepublic of Korea

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