Human Cell

, Volume 33, Issue 1, pp 291–293 | Cite as

A new method to identify fetal sex and trisomy 21 from the amniocentesis of pregnant women

  • Junbei Xiang
  • Qian WanEmail author
  • Mianxue Liu
  • Ruifeng Wang
Letter to the Editor

Dear Sir,

There are several methods to identify the sex of fetuses:

Imaging identification with B-mode ultrasound [1]: after 15 weeks of gestation, ordinary B-mode ultrasound, three-dimensional color ultrasound, or four-dimensional color ultrasound can identify the sex of fetuses because at that time, there is obvious difference in the reproductive organ development between male and female fetuses. This method is time-window-restricted and requires skillful operator.

Microscopic karyotype identification method [2]: the amniotic fluid in the uterus of pregnant women was obtained by amniocentesis technology, and fetal cells were isolated from the obtained amniotic fluid, cultured, and then the chromosome karyotype of fetal cells was analyzed. The sex chromosomes of the fetus were observed under the microscope to identify the sex type of fetuses. This method lasts 10 days, requires skillful operator and is labor intensive.

DNA detection method [3, 4, 5]: the fetal amniotic fluid or fetal...



This study was supported by Scientific Research Program of Sichuan Health Commission of China (No. 16PJ404) and the Foundation of Sichuan Nursing Vocation College of China (No. 2017ZRZ04).

Compliance with ethical standards

Conflict of interest

We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work. There is no professional or other personal interest of any nature or kind in any product, service, and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled, “A new method to identify fetal sex and trisomy 21 from the amniocentesis of pregnant women”.

Supplementary material

13577_2019_311_MOESM1_ESM.doc (137 kb)
Supplementary material 1 (DOC 137 kb)


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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Sichuan Nursing Vocational CollegeChengduPeople’s Republic of China
  2. 2.Chengdu Neo-life Hope Medical Lab. Co. LtdChengduPeople’s Republic of China
  3. 3.Sichuan Institute of Atomic Energy/Irradiation Preservation Key Laboratory of Sichuan ProvinceChengduPeople’s Republic of China

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