Human Cell

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Spermatogenesis-associated changes of fucosylated glycolipids in murine testis

  • Masao IwamoriEmail author
  • Shigeki Adachi
  • Bei Lin
  • Kyoko Tanaka
  • Daisuke Aoki
  • Taisei Nomura
Research Article


By targeted deletion of either the FUT1- or FUT2-gene for α1,2-fucosyltransferase, expression of FGM1 and FGA1, in murine testis was revealed to be sustained through unique interchangeability of the genes, indicating their significant roles for spermatogenesis. Accordingly, we examined the amounts of FGM1 and FGA1 in the testes of mice at 1–42 days after birth in comparison to those of several glycolipids including seminolipid. Although Forssman antigen and GM1 were present in relatively constant amounts during the period examined, GM3, which was the major one at 1 day, quickly decreased during development and had completely disappeared at 4 weeks. The following glycolipids were expressed in stage-specific manners, FGM1 for primary spermatocytes at 1 week, a seminolipid for secondary spermatocytes at 2 weeks, and GM3 lactone and FGA1 for spermatids and spermatozoa at 3 weeks. In fact, immunohistochemical staining with anti-FGM1 and anti-FGA1 antibodies demonstrated that FGM1 and FGA1 were distributed in the spermatocytes, and the spermatids and spermatozoa, respectively, and FGA1, together with seminolipid, were the immunogenic markers of spermatozoa. Thus, the fucosylation of glycolipids is a spermatogenesis-associated event, which should occur even with use of either the FUT1- or FUT2-gene.


Fucosylated glycolipids Seminolipid Gangliosides Spermatogenesis 



This work was supported in part by a grant-in-aid for scientific research from the Japanese Ministry of Education, Culture, Sports, Science and Technology (Grant no. 17K11295, Kyoko Tanaka).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Masao Iwamori
    • 1
    Email author
  • Shigeki Adachi
    • 1
  • Bei Lin
    • 2
  • Kyoko Tanaka
    • 3
  • Daisuke Aoki
    • 4
  • Taisei Nomura
    • 1
  1. 1.Animal and Human Model Project for Healthcare and Drug Development (Nomura Project), National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN)IbarakiJapan
  2. 2.Department of Obstetrics and GynecologyShengjing Hospital of China Medical UniversityShenyangPeople’s Republic of China
  3. 3.Department of Obstetrics and GynecologyOhashi Hospital, Toho UniversityTokyoJapan
  4. 4.Department of Obstetrics and Gynecology, School of MedicineKeio UniversityTokyoJapan

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