Human Cell

, Volume 33, Issue 1, pp 261–271 | Cite as

MiR-490-5p functions as tumor suppressor in childhood neuroblastoma by targeting MYEOV

  • Jinfeng Wang
  • Xin ZhangEmail author
  • Hongyue Yao
  • Yuan Le
  • Wei Zhou
  • Jing Li
  • Lingling Lu
  • Meixue Chen
  • Xiang Li
Research Article


Neuroblastoma (NB) is the most common extracranial solid tumor derived from neural crest in children. Recently, the role of miRNA has been studied extensively in the development of NB. Here, we investigated the clinical significance of microRNA-490-5p (miR-490-5p) in NB. A total of 72 pairs of NB tumor tissues and matched adjacent normal nerve tissues were collected from NB patients. The expression of miR-490-5p was significantly down-regulated in NB tissues and cell lines using quantitative real-time PCR. Using Pearson Chi-square test and Kaplan–Meier analysis, we found that significantly decreased miR-490-5p levels were correlated with INSS stage, lymph-node metastasis, and poor survival prognosis in NB patients. MiR-490-5p overexpression significantly suppressed cell proliferation migration, invasion, and induced cell cycle G0/G1 arrest and cell apoptosis in NB cell lines (SH-SY5Y and SK-N-SH) using CCK-8, flow cytometry, and transwell assays. Mechanistically, MYEOV was confirmed as a target gene of miR-490-5p by luciferase reporter assay. Furthermore, MYEOV knockdown imitated, while overexpression rescued the changes in the biological features of miR-490-5p on NB cells. Our results demonstrated for the first time that miR-490-5p functions as a tumor suppressor in NB by targeting MYEOV, which might provide novel approaches for the treatment of NB.


Neuroblastoma miR-490-5p MYEOV Prognosis Tumor suppressor 



Not applicable.

Compliance with ethical standards

Conflict of interest

The authors have declared no conflict of interest.

Ethics approval and consent to participate

All the protocols in this study were approved by the Ethics Committee of Jinzhou Medical University First Affiliated Hospital (Approval number: FM2314-124).

Consent for publication

Not applicable.


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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2020

Authors and Affiliations

  • Jinfeng Wang
    • 1
  • Xin Zhang
    • 2
    Email author
  • Hongyue Yao
    • 1
  • Yuan Le
    • 1
  • Wei Zhou
    • 1
  • Jing Li
    • 1
  • Lingling Lu
    • 1
  • Meixue Chen
    • 1
  • Xiang Li
    • 1
  1. 1.Department of PediatricsJinzhou Medical University First Affiliated HospitalJinzhouChina
  2. 2.Department of OncologyJinzhou Medical University First Affiliated HospitalJinzhouChina

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