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Human Cell

, Volume 33, Issue 1, pp 131–139 | Cite as

LncRNA LINC00470 promotes proliferation through association with NF45/NF90 complex in hepatocellular carcinoma

  • Wenwei Huang
  • Juntao Liu
  • Jijun Yan
  • Zhengbin Huang
  • Xiongjie Zhang
  • Yanping Mao
  • Xiufang HuangEmail author
Research Article
  • 74 Downloads

Abstract

Increasing evidence demonstrates that long noncoding RNAs (lncRNAs) play an important role in the development and progression of human cancers. LncRNA LINC00470 has been reported to function as an oncogene in glioblastoma. Until now, the roles and underlying mechanisms of LINC00470 in the progression of hepatocellular carcinoma (HCC) remain unclear. Here, we found that LINC00470 was upregulated in HCC cells and tissues. High-level LINC00470 was significantly correlated with bigger tumor size, advanced TNM stage and poor prognosis in patients with HCC. Functional studies showed that knockdown of LINC00470 expression inhibited HCC cell proliferation and cell cycle progression, while overexpression of LINC00470 showed the opposite effects. Further investigation suggested that LINC00470 was associated with NF45/NF90 complex and increased its interaction with cyclin E1 mRNA, thus inhibiting the degradation of cyclin E1 mRNA. Additionally, knockdown of cyclin E1 in LINC00470-overexpressed cells abolished its promotive effects on HCC cell proliferation. In summary, our findings suggest that targeting LINC00470 could be a potential therapeutic approach in treating HCC patients.

Keywords

Cyclin E1 mRNA stability RNA-binding protein Cell cycle 

Notes

Author contributions

All the experiments were conducted by WH, JL, JY, ZH and XZ. YM and XH designed this research and finished the manuscript.

Compliance with ethical standards

Conflict of interest

All the authors declare that they have no conflict of interest.

Ethical approval

The study was approved by the research ethics committee of Hanchuan People’s Hospital and conducted in accordance with the Declaration of Helsinki (approval number: 2019082301). Written informed consent was obtained from all HCC patients.

Supplementary material

13577_2019_288_MOESM1_ESM.tif (941 kb)
Supplemental Fig. 1. Biotin RNA pull-downs were performed with cell extracts of cells using full-length LINC00470 transcript. This was followed by mass spectrometry. (TIFF 940 kb)

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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Wenwei Huang
    • 1
  • Juntao Liu
    • 1
  • Jijun Yan
    • 1
  • Zhengbin Huang
    • 1
  • Xiongjie Zhang
    • 1
  • Yanping Mao
    • 1
  • Xiufang Huang
    • 1
    Email author
  1. 1.Department of General SurgeryHanchuan People’s HospitalHanchuan CityChina

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