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Human Cell

pp 1–7 | Cite as

MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis

  • Li WangEmail author
  • Xin Yang
  • Wencai Li
  • Xicheng SongEmail author
  • Shasha KangEmail author
Research Article
  • 32 Downloads

Abstract

Regulatory T cells (Tregs) play a crucial role in allergic rhinitis (AR). However, the mechanism of how Tregs are regulated in AR is poorly understood. Here, we aimed to explore the role of Tregs in AR and how Tregs were regulated by miR-202-5P, which was demonstrated to be important in AR. Peripheral blood mononuclear cells (PBMC) were isolated from collected blood samples. Tregs were purified using Regulatory T Cell Isolation Kit, and differentiated from isolated CD4 T cells using recombinant human interleukin-2 (rhIL-2) and transforming growth factor beta (TGF-β). mRNA expression levels of miR-202-5p, matrilin-2 (MATN2), TGF-β1 and interleukin-10 (IL-10) were detected by real-time PCR. The concentrations of IL-4, interleukin-17 (IL-17), IL-10, interferon gamma (IFN-γ) and TGF-β1 were detected by enzyme-linked immunosorbent assay (ELISA). MATN2 protein level was detected by Western blot. MiR-202-5p expression dramatically elevated in PBMCs, CD4+ T cells and Tregs of AR patients. In vitro, miR-202-5p promoted Tregs differentiation via targeting MATN2. MiR-202-5p/MATN2 axis mediated Tregs proliferation and functions. MiR-202-5p/MATN2 are associated with regulatory T-cells differentiation and function in allergic rhinitis.

Keywords

MiR-202-5p MATN2 Tregs AR Differentiation 

Notes

Acknowledgements

None.

Funding

The study was supported by the National Natural Science Foundation of China (81670907).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

The procedures were approved by the human ethics committee of Yuhuangding Hospital (#YHDH1305LC148).

Informed consent

Informed consent has been derived from each participant.

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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Otorhinolaryngology Head and Neck SurgeryYuhuangding HospitalYantaiChina
  2. 2.Department of Cardiovascular MedicineYuhuangding HospitalYantaiChina

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