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Human Cell

, Volume 32, Issue 3, pp 390–396 | Cite as

miR-376a inhibits the proliferation and invasion of osteosarcoma by targeting FBXO11

  • Qiaolong Xu
  • Li Cheng
  • Jianyang ChenEmail author
  • Wenjie Lu
  • Peinian Wang
Research Article
  • 59 Downloads

Abstract

Dysregulation of microRNAs (miRNAs) is frequently found in the tumorigenesis of osteosarcoma (OS). miR-376a was found to play tumor suppressive roles in human cancers. However, the role of miR-376a in OS remains unclear. The expression of miR-376a was analyzed by quantitative real-time PCR (qRT-PCR) in OS cell lines. Cell proliferation assay, cell invasion assay, and cell apoptosis assay were performed to detect the biological function of miR-376a after synthetic miRNA transfection. The target of miR-376a was predicted by TargetScan and miRDB, and further validated by luciferase activity reporter assay and western blot. miR-376a expression was revealed to be decreased in OS cell lines. In vitro experiments showed that overexpression of miR-376a inhibits OS cell proliferation and invasion but promotes apoptosis. Luciferase activity reporter assay and western blot assay showed F-box protein 11 (FBXO11) was a direct target of miR-376a. Furthermore, FBXO11 mediated the role of miR-376a on the proliferation, invasion, and apoptosis of OS cells. Collectively, these results revealed miR-376a functions as a tumor suppressor by targeting FBXO11 in OS. It may be developed as a therapeutic target for OS patients.

Keywords

miR-376a FBXO11 Osteosarcoma Tumor suppressive miRNA 

Notes

Author contributions

All the authors carried out the study and the data statistics. All the authors participated in study design, scientific discussion of the data, and drafted the manuscript.

Funding

None.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Data availability

All data are included in this published article. Any additional information related to this study is available from the author for correspondence upon reasonable request.

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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Qiaolong Xu
    • 1
  • Li Cheng
    • 1
  • Jianyang Chen
    • 1
    Email author
  • Wenjie Lu
    • 1
  • Peinian Wang
    • 1
  1. 1.Department of OrthopedicThe People’s Hospital of CixiCixiPeople’s Republic of China

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