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Human Cell

, Volume 32, Issue 3, pp 326–333 | Cite as

MicroRNA-30e inhibits proliferation and invasion of non-small cell lung cancer via targeting SOX9

  • Yanwei Cui
  • Lei Zhao
  • Shilei Zhao
  • Tao Guo
  • Fengzhou Li
  • Zhuoshi Li
  • Lei Fang
  • Taihua WuEmail author
  • Chundong GuEmail author
Research Article

Abstract

Previous studies have reported that microRNA-30e (miR-30e) is dysregulated in multiple human cancers. However, the expression, functions and molecular mechanism of miR-30e in NSCLC remain unknown. In this study, we found that miR-30e was expressed at a low level in NSCLC tissues and cell lines. In NSCLC cell lines, enforced expression of miR-30e could inhibit cell proliferation and invasion in vitro. In addition, miR-30e negatively regulated SOX9 expression through directly binding to the 3′UTR of SOX9, and an inverse correlation was found between miR-30e and SOX9 mRNA expression in NSCLC tissues. Moreover, knockdown of SOX9 led to decreased proliferation and invasion of NSCLC cells. Taken together, miR-30e acts as a tumor suppressor in NSCLC, and inhibits cell proliferation and invasion possibly by directly targeting SOX9. These findings might provide novel therapeutic targets for NSCLC.

Keywords

MicroRNA-30e Proliferation Invasion SOX9 Non-small cell lung cancer 

Notes

Acknowledgements

This work was supported by a grant from the National Nature Science Foundation of China (181774078, 81773453, 81803886), National Nature Science Foundation of Liaoning Province, China (201602227, 20170540300).

Compliance with ethical standards

Conflict of interest

All the authors declared no conflicts of interest in this work.

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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2019

Authors and Affiliations

  • Yanwei Cui
    • 1
    • 2
  • Lei Zhao
    • 1
    • 2
  • Shilei Zhao
    • 1
    • 2
  • Tao Guo
    • 1
    • 2
  • Fengzhou Li
    • 1
    • 2
  • Zhuoshi Li
    • 1
    • 2
  • Lei Fang
    • 1
    • 2
  • Taihua Wu
    • 3
    Email author
  • Chundong Gu
    • 1
    • 2
    Email author
  1. 1.Department of Thoracic SurgeryThe First Affiliated Hospital of Dalian Medical UniversityDalianChina
  2. 2.Lung Cancer Diagnosis and Treatment Center of DalianThe First Affiliated Hospital of Dalian Medical UniversityDalianChina
  3. 3.Departments of Respiratory MedicineThe First Affiliated Hospital of Dalian Medical UniversityDalianChina

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