Human Cell

, Volume 32, Issue 1, pp 75–82 | Cite as

Establishment and characterization of human gingival squamous cell carcinoma cell line NOCS-1

  • Haruka TakahashiEmail author
  • Miho Watanabe
  • Akihiro Ohyama
  • Junko Toyomura
  • Toshiaki Tachibana
  • Akira Tanaka
  • Hiroshi Ishikawa
Cell Line


The NOCS-1 cell line was established from the left gingiva tumor in an 86-year-old Japanese man. Histopathological diagnosis of the original tumor was well-differentiated squamous cell carcinoma. NOCS-1 cells were adhesive epithelial cells with neoplastic or pleomorphic features and grew without contact inhibition. It has been subcultured 70 times during the past 26 months. From passage 3, melanin-containing cells began to be observed in the NOCS-1 cell line. The plating efficiencies were 25% and 23%, doubling times were 29 and 26 h, and saturation densities were 6.9 × 104/cm2 and 8.7 × 104/cm2, at passage 12 and 30, respectively. When NOCS-1 cells were xenotransplanted subcutaneously into SCID mice, they produced tumors that histopathologically resembled the original tumor. In addition, NOCS-1-XG cells derived from the xenotransplanted tumor were similar to NOCS-1 cells. We believe that this cell line may be a valuable tool to develop immunotherapy and chemotherapy regimens.


Cell line Gingival squamous cell carcinoma G establishment Xenograft Anticancer drug susceptibility tests 



We thank M. Arico from Edanz Group ( for editing a draft of this manuscript.

Compliance with ethical standards

Conflict of interest

No competing financial interests exist. The authors declare that there are no conflicts of interest related to the publication of this study.


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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Oral and Maxillofacial SurgeryNippon Dental University School of Life Dentistry at NiigataNiigataJapan
  2. 2.Department of NDU Life SciencesNippon Dental University School of Life Dentistry at TokyoTokyoJapan
  3. 3.Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of MedicineUniversity of TsukubaTsukubaJapan
  4. 4.Core Research FacilitiesThe Jikei University School of MedicineTokyoJapan

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