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Human Cell

, Volume 32, Issue 2, pp 185–192 | Cite as

Diversity of cell phenotypes among MT-2 cell lines affects the growth of U937 cells and cytokine production

  • Hajime Nomura
  • Kunihiko UmekitaEmail author
  • Yuuki Hashikura
  • Kazumi Umeki
  • Ikuo Yamamoto
  • Yatsuki Aratake
  • Mineki Saito
  • Hiroo Hasegawa
  • Katsunori Yanagihara
  • Akihiko Okayama
Research Article
  • 30 Downloads

Abstract

We previously reported the diversity of structure and integration sites of human T-cell leukemia virus type 1 (HTLV-1) provirus among different MT-2 cell lines. This raised the question as to whether cell phenotypes also differed among MT-2 cell lines. The influence of two different MT-2 cell lines (MT-2J and MT-2B) on the growth of the promonocytic leukemia cell line, U937, was investigated. Protein levels and mRNA expression of cytokines were also investigated. In addition, Western blot analysis of HTLV-1 regulatory proteins, Tax and HBZ, was also performed. Culture supernatant from MT-2B, but not MT-2J, cells showed marked suppressive effects on U937 cell growth. MT-2B showed high tumor necrosis factor (TNF)-α, TNF-β, and interferon (IFN)-γ both in protein levels of the culture supernatant and mRNA levels of the cells. Analysis using recombinant cytokines indicated that the suppressive effects of MT-2B were due, at least in part, to high levels of TNF-β and its synergic effects with IFN-γ in the culture supernatant. Protein levels of HTLV-1 Tax and HBZ were higher in MT-2B than those in MT-2J cells. These molecules have been reported to affect the cytokine production of HTLV-1 infected cells; therefore, the difference in these molecules may have accounted for the differences in cytokine production between MT-2J and MT-2B cells. Furthermore, because MT-2 cells showed a large variation of integrated HTLV-1 proviruses as well as cell phenotypes, it is important to exercise caution in the assessment and interpretation of experimental data from MT-2 cells.

Keywords

HTLV-1 Cytokine production MT-2 cell lines Tumor necrosis factor Interferon gamma 

Notes

Acknowledgements

The authors would like to thank Ms Y. Kaseda (Miyazaki University) for her technical support and Dr Y. Ohkuwa (Miyazaki University) for his instruction concerning the statistical analysis. The authors would like to thank Enago (http://www.enago.jp) for the English language review.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving human participants and/or animals

This article does not contain any studies with human participants or animals performed by any of the authors.

Informed consent

Not applicable.

Supplementary material

13577_2018_231_MOESM1_ESM.doc (92 kb)
Supplementary material 1 (DOC 92 KB)

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Copyright information

© Japan Human Cell Society and Springer Japan KK, part of Springer Nature 2018

Authors and Affiliations

  • Hajime Nomura
    • 1
  • Kunihiko Umekita
    • 1
    Email author
  • Yuuki Hashikura
    • 1
  • Kazumi Umeki
    • 1
  • Ikuo Yamamoto
    • 1
  • Yatsuki Aratake
    • 2
  • Mineki Saito
    • 3
  • Hiroo Hasegawa
    • 4
  • Katsunori Yanagihara
    • 4
  • Akihiko Okayama
    • 1
  1. 1.Department of Rheumatology, Infectious Diseases and Laboratory Medicine, Faculty of MedicineUniversity of MiyazakiKiyotakeJapan
  2. 2.Department of Life MedicalKyushu University of Health and WelfareNobeokaJapan
  3. 3.Department of MicrobiologyKawasaki Medical SchoolKurashikiJapan
  4. 4.Department of Laboratory MedicineNagasaki University Graduate School of Biomedical SciencesNagasakiJapan

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