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Expression study of five genes involved in floral organ development in multiple seeded rice

  • Soumya Prakash Das
  • Debal Deb
  • Narottam DeyEmail author
Short Communication

Abstract

Rice cultivar Jugal is a unique floral organ mutant from South Bengal and Odisa, the two sister states of Eastern India, carries more than one kernels in most of its spikelet. Most of the mature florets of this line possess more than one carpal which later developed into more than one kernels within a single grain on maturity. In order to study the role of floral organ development genes commonly involved, expression study of five selected floral organ developmental genes (OsMADS3, OsMADS13, OsMADS21, OsMADS58, and DL) were studied through real time based quantitative PCR for three consecutive flower organ developmental stages (Sp5, Sp6, and Sp7) with reference to a normal rice line (IR36). All the studied genes showed differential relative expression in respect to the reference gene both in mutant and normal rice lines for the studied genes and stages and individual distinct pattern except DL gene which was almost similar in both Jugal and IR36 at early stage of floral organ development viz Sp5 and Sp6 stage. However, after Sp6 stage the expression is reduced in the normal rice (IR36) but in case of the mutant rice (Jugal) the expression started to increase and at Sp7 the expression level was much higher in the mutant line. The information resulted from the investigation form the basic idea on regulatory aspects of floral organ development in rice.

Keywords

Multiple seeded rice Floral organ mutant OsMADS DL Relative gene expression 

Abbreviations

AG

Agamous

CRC

Crabs claw

DL

Drooping leaf

OsMADS

Oryza sativa MADS-box

Notes

Acknowledgements

The work was financially supported by SERB, Department of Science and Technology, Government of India in form of a research project (Ref. No. SB/YS/LS-187/2013).

References

  1. Alvarez J, Smyth DR (1999) CRABS CLAW and SPATULA, two Arabidopsis genes that control carpel development in parallel with AGAMOUS. Development 126:2377–2386Google Scholar
  2. Bommert P, Satoh-Nagasawa N, Jackson D, Hirano HY (2005) Genetics and evolution of inflorescence and flower development in grasses. Plant Cell Physiol 46:69–78CrossRefGoogle Scholar
  3. Das SP, Deb D, Dey N (2018) Micromorphic and molecular studies of floral organs of a multiple seeded rice. Plant Mol Biol Report. 1:1.  https://doi.org/10.1007/s11105-018-1116-9 Google Scholar
  4. Dreni L, Kater MM (2014) MADS reloaded: evolution of the AGAMOUS subfamily genes. New Phytol 201:717–732CrossRefGoogle Scholar
  5. Dreni L, Pilatone A, Yun D, Erreni S, Pajoro A, Caporali E, Zhang D, Katera MM (2011) Functional analysis of all AGAMOUS subfamily members in rice reveals their roles in reproductive organ identity determination and meristem determinacy. Plant Cell 23:2850–2863CrossRefGoogle Scholar
  6. Hu Y, Liang W, Yin C, Yang X, Ping B, Li A, Jia R, Chen M, Luo Z, Cai Q, Zhao X, Dabing Zhang D, Yuan Z (2015) Interactions of OsMADS1 with floral homeotic genes in rice flower development. Mol Plant 8(9):1366–1384CrossRefGoogle Scholar
  7. Ikeda K, Sunohara H, Nagato Y (2004) Developmental course of inflorescence and spikelet in rice. Breed Sci 54:147–156CrossRefGoogle Scholar
  8. Kellogg EA (2001) Evolutionary history of the grasses. Plant Physiol 125:1198–1205CrossRefGoogle Scholar
  9. Kramer EM, Jaramillo MA, Di Stilio VS (2004) Patterns of gene duplication and functional evolution during the diversification of the AGAMOUS subfamily of MADS box genes in angiosperms. Genetics 166:1011–1023CrossRefGoogle Scholar
  10. Kyozuka J, Kobayashi T, Morita M, Shimamoto K (2000) Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B and C genes. Plant Cell Physiol 41:710–718CrossRefGoogle Scholar
  11. Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y (2003) SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development 130:705–718CrossRefGoogle Scholar
  12. Pandian RT, Thiyagarajan K (2004) Inheritance of floral traits in spontaneous mutant in rice (Oryza sativa L.). Curr Sci 87:1051–1052Google Scholar
  13. Parthasarathy N (1936) The inheritance of multiple pistils in rice. Proc Assoc Econ Biol Coimbatore 3:32–41Google Scholar
  14. Prain D (1903) Bengal Plants. Bot Surv India Calcutta 2:1184Google Scholar
  15. Priya A, Das SP, Goswami S, Adak MK, Deb D, Dey N (2015) An exploratory study on allelic diversity for five genetic loci associated with floral organ development in rice. Am J Plant Sci 6:1973–1980CrossRefGoogle Scholar
  16. Soltis DE, Chanderbali AS, Kim S, Buzgo SM, Soltis PS (2007) The ABC model and its applicability to basal angiosperms. Ann Bot 100:155–163CrossRefGoogle Scholar
  17. Yamaguchi T, Nagasawa Kawasaki S, Matsuoka M, Nagato Y, Hirano HY (2004) The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa. Plant Cell 16:500–509CrossRefGoogle Scholar
  18. Yamaguchi T, Lee DY, Miyao A, Hirochika H, An G, Hirano HY (2006) Functional diversification of the two C-class genes OsMADS3 and OsMADS58 in Oryza sativa. Plant Cell 18:15–28CrossRefGoogle Scholar
  19. Zanis MJ (2007) Grass spikelet genetics and duplicate gene comparisons. Int J Plant Sci 168:93–110CrossRefGoogle Scholar

Copyright information

© Society for Plant Biochemistry and Biotechnology 2019

Authors and Affiliations

  1. 1.Rice Biotechnology Laboratory, Department of BiotechnologyVisva-BharatiSantiniketanIndia
  2. 2.Centre for Interdisciplinary StudiesBasudha Biotechnology Laboratory for Conservation (Basudha Trust)KolkataIndia

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