Expression study of five genes involved in floral organ development in multiple seeded rice

  • Soumya Prakash Das
  • Debal Deb
  • Narottam DeyEmail author
Short Communication


Rice cultivar Jugal is a unique floral organ mutant from South Bengal and Odisa, the two sister states of Eastern India, carries more than one kernels in most of its spikelet. Most of the mature florets of this line possess more than one carpal which later developed into more than one kernels within a single grain on maturity. In order to study the role of floral organ development genes commonly involved, expression study of five selected floral organ developmental genes (OsMADS3, OsMADS13, OsMADS21, OsMADS58, and DL) were studied through real time based quantitative PCR for three consecutive flower organ developmental stages (Sp5, Sp6, and Sp7) with reference to a normal rice line (IR36). All the studied genes showed differential relative expression in respect to the reference gene both in mutant and normal rice lines for the studied genes and stages and individual distinct pattern except DL gene which was almost similar in both Jugal and IR36 at early stage of floral organ development viz Sp5 and Sp6 stage. However, after Sp6 stage the expression is reduced in the normal rice (IR36) but in case of the mutant rice (Jugal) the expression started to increase and at Sp7 the expression level was much higher in the mutant line. The information resulted from the investigation form the basic idea on regulatory aspects of floral organ development in rice.


Multiple seeded rice Floral organ mutant OsMADS DL Relative gene expression 





Crabs claw


Drooping leaf


Oryza sativa MADS-box



The work was financially supported by SERB, Department of Science and Technology, Government of India in form of a research project (Ref. No. SB/YS/LS-187/2013).


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Copyright information

© Society for Plant Biochemistry and Biotechnology 2019

Authors and Affiliations

  1. 1.Rice Biotechnology Laboratory, Department of BiotechnologyVisva-BharatiSantiniketanIndia
  2. 2.Centre for Interdisciplinary StudiesBasudha Biotechnology Laboratory for Conservation (Basudha Trust)KolkataIndia

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