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Biochemical estimation of Moringa oleifera leaf extract for synthesis of silver nanoparticle mediated drug delivery system

  • Samrat Paul
  • Piyali BasakEmail author
  • Ranabir Majumder
  • Anwesha Mukherjee
  • Joydeep Ghosh
  • Shamayita Patra
  • Nandan Kumar Jana
Original Article
  • 23 Downloads

Abstract

Breast tissue remodeling occurs throughout a women’s life from pre-menstrual to post menopausal time, hence there is a high risk of DNA damage. Estrogen metabolite generates free radical that causes DNA damage leading to cancer cell formation. Moringa oleifera leaf extract contain rich amount of Polyphenolic and Flavonoids compound which trap the free radical and thereby prevent cancer cell progression. Moreover anti-gonadotrophic hormonal activity of the leaf extract slows down the proliferation rate of T47D cell line. Hence our aim of study mainly focused to develop an anticancer drug delivery system using M. oleifera leaf extract. Silver nanoparticle (AgNp) is formed by green synthesis method using M. oleifera leaf extract. Reducing sugar, Flavonoids, Polyphenolic compound, and NADPH dependent dehydrogenase present in M. oleifera leaf extract are responsible for reduction of the silver ion (Ag+) followed by formation of metallic silver (Ag0). The formation of spherical and rectangular shaped AgNp with average size of 50 nm is reflected with sharp SPR band near 420 nm and from SEM, TEM analysis. The nanoparticle mediated polyvinyl alcohol composite film (nano-rod) is prepared for delivery of the purified anti-neoplastic phyto-compound from leaf extract. The large surface area of AgNp permits its coordination with purified compound molecule by metal ligand bonding. Therefore it provide duel effect, it acts as carrier molecule for phyto-compound and also creates cytotoxicity effect on cancer cells. Single dose treatment on HeLa cell indicates that the composite film is more effective in killing the cancer cell than the purified anti-neoplastic phyto-compound.

Keywords

Moringa oleifera Biochemical analysis Green synthesis PVA-AgNp composite Anti estrogenic hormonal screening Breast tissue remodeling 

Abbreviations

AgNp

Silver nanoparticle

PVA

Polyvinyl alcohol

SPR

Surface plasmon resonance

ROS

Reactive oxygen species

Notes

Acknowledgements

I convey my heartiest gratitude to Department of Physics, and School of material science and Nanotechnology, Jadavpur University for their kind of help and cooperation. I am grateful to Department of Science and Technology –West Bengal for Research support and Sponsoring the Project with a Grant No. 45(Sanc.)/ST/P/S&T/9G-6/2014.

Compliance with ethical standards

Conflict of interest

The authors declare there is no conflict of interest regarding the manuscript.

Supplementary material

13562_2019_517_MOESM1_ESM.doc (199 kb)
Supplementary material 1 (DOC 199 kb)

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Copyright information

© Society for Plant Biochemistry and Biotechnology 2019

Authors and Affiliations

  • Samrat Paul
    • 1
  • Piyali Basak
    • 1
    Email author
  • Ranabir Majumder
    • 1
  • Anwesha Mukherjee
    • 2
  • Joydeep Ghosh
    • 1
  • Shamayita Patra
    • 1
  • Nandan Kumar Jana
    • 2
  1. 1.School of Bioscience and EngineeringJadavpur UniversityKolkataIndia
  2. 2.Department of BiotechnologyHeritage Institute of TechnologyKolkataIndia

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