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Isolation and functional analysis of FLOWERING LOCUS T orthologous gene from Vanda hybrid

  • Kanokwan Panjama
  • Eriko Suzuki
  • Masahiro Otani
  • Masaru Nakano
  • Norikuni Ohtake
  • Takuji Ohyama
  • Weenun Bundithya
  • Kuni Sueyoshi
  • Soraya RuamrungsriEmail author
Original Article
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Abstract

Vanda is an important ornamental tropical orchid in Thailand and shows great potential in international markets. Since there has been limited research on flowering processes and regulations, flower production programs cannot be properly managed. To understand the genetic mechanisms in the Vanda flowering process, the ortholog of the FLOWERING LOCUS T (FT) gene (VaFT) was isolated and characterized by using Vanda ‘Ratchaburi-Fusch Katsura’. An open reading frame of 531 bp, translating a protein of 176 amino acids (AAs), was obtained from VaFT. The AA sequence alignment of VaFT indicated that it contains a conserved domain, distinctive to the phosphatidylethanolamine-binding proteins (PEBPs) superfamily, and shares high homology with other orchid FT proteins: 93% of PaFT from Phalaenopsis aphrodite, 91% of CgFT from Cymbidium goeringii and 89% of OnFT from Oncidium Gower Ramsey. Ectopic expression of VaFT in transgenic Arabidopsis resulted in activation of floral meristem identity gene APETALA1 (AP1) and early flowering with fewer rosette leaves than non-transgenic Arabidopsis. Our data suggests that VaFT is apparently a PEBPs gene in orchids that conducts the transition of flowering.

Keywords

Cloning FLOWERING LOCUS T (FT) Gene expression Orchid Vanda 

Abbreviations

FT

FLOWERING LOCUS T

PEBPs

Phosphatidylethanolamine-binding proteins

RT-PCR

Reverse transcription PCR

RACE

The rapid amplification of cDNA ends

AAs

Amino acids

Notes

Acknowledgements

We appreciate the contribution of Magic Farm, Ratchaburi Province, Thailand, which provided plant materials. We would also like to thank the Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University and H.M. the King’s Initiative Centre for Flower and Fruit Propagation, Thailand for providing accommodations.

Compliance with ethical standards

Conflicts of interest

The authors declare that we have no conflicts of interest.

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Copyright information

© Society for Plant Biochemistry and Biotechnology 2019

Authors and Affiliations

  • Kanokwan Panjama
    • 1
    • 2
  • Eriko Suzuki
    • 1
  • Masahiro Otani
    • 1
  • Masaru Nakano
    • 1
  • Norikuni Ohtake
    • 1
  • Takuji Ohyama
    • 1
  • Weenun Bundithya
    • 2
  • Kuni Sueyoshi
    • 1
  • Soraya Ruamrungsri
    • 2
    Email author
  1. 1.Graduate School of Science and TechnologyNiigata UniversityNiigataJapan
  2. 2.Department of Plant and Soil Sciences, Faculty of AgricultureChiang Mai UniversityChiang MaiThailand

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