Validation of QTL for grain weight using MAS-derived pairs of NILs in bread wheat (Triticum aestivum L.)

  • Supriya Kumari
  • Reyazul Rouf Mir
  • Sandhya Tyagi
  • Harindra Singh Balyan
  • Pushpendra Kumar GuptaEmail author
Original Article


Near isogenic lines (NILs) are ideal material for a variety of genetic studies including validation of specific QTL. In the present study, eight pairs of NILs for grain weight were developed, seven in the background of Raj3765, and one in the background of K9107. For this purpose, marker-assisted selection (MAS) was used for the transfer of three grain weight QTL (QGw.ccsu-1A.2, QGw.ccsu-1A.3 and QGw.ccsu-1B.1) that were earlier identified in our laboratory. Two genotypes of each of the eight pairs of NILs, differed for QTL alleles (QTLHgw derived from the donor parent and the QTLLgw derived from the recipient parent). Each pair of NILs involved a solitary QTL except one NIL, which differed for all the three QTL. The difference in thousand grain weight (TGW) in two NILs of an individual pair ranged from 2.8 to 7.5 g, thus validating the effect of the QTL for TGW, although the quantum of difference did not always match the phenotypic variance of the corresponding QTL. As expected, the NILs which involved all the three QTL had the maximum difference of 7.5 g in TGW, and the NILs which involved QTL, QGw.ccsu-1A.2 had minimum average difference of 2.8 g for TGW. The NILs produced during the present study may be used in future for MAS and for fine mapping of TGW QTL.


Near isogenic line Thousand grain weight Quantitative trait loci Triticum aestivum L. SSR marker 



Back crossed


Chandrashekhar Azad University of Agriculture and Technology


Doubled haploid


Days to maturity


Harvest index per plant


Marker-assisted selection


Near isogenic line


Phenotypic variance explained


Quantitative trait loci


Donor parent high GW QTL allele


Recipient parent low GW QTL allele


Rajasthan Agriculture University



The authors like to thank The Head, Department of Genetics and Plant Breeding, CCS University (Meerut, India) for providing facilities. PKG and HSB were each awarded the position of INSA Senior Scientist. PKG was also awarded a National Academy of Sciences India (NASI) Senior Scientist Platinum Jubilee Fellowship during the tenure of this research work. SK was awarded a JRF/SRF in a research project funded by the Department of Biotechnology, Government of India, New Delhi. Thanks are also due to Mr. Saripalli Gautam for his help in preparing the figures.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13562_2018_485_MOESM1_ESM.docx (260 kb)
Supplementary material 1 (DOCX 261 kb)


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Copyright information

© Society for Plant Biochemistry and Biotechnology 2019

Authors and Affiliations

  • Supriya Kumari
    • 1
  • Reyazul Rouf Mir
    • 2
  • Sandhya Tyagi
    • 2
  • Harindra Singh Balyan
    • 1
  • Pushpendra Kumar Gupta
    • 1
    Email author
  1. 1.Molecular Biology Laboratory, Department of Genetics and Plant BreedingChaudhary Charan Singh UniversityMeerutIndia
  2. 2.Division of Genetics and Plant BreedingSher-e-Kashmir University of Agricultural Sciences and Technology of KashmirSrinagarIndia

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