Application of PCR based diagnostics in the exploration of Parastagonospora nodorum prevalence in wheat growing regions of Himachal Pradesh

  • S. Katoch
  • S. K. Rana
  • P. N. SharmaEmail author
Original Article


Stagonospora leaf and glume blotch (SLGB) of wheat caused by Parastagonospora nodorum (formerly Stagonospora nodorum) has recently emerged as a major problem in changing climatic conditions of Himachal Pradesh (HP), especially during delayed winter rains. In the present studies symptomatology, morpho-cultural as well as molecular marker based identification showed the prevalence of disease in the state and conclusively proved that leaf and glume blotch of wheat is caused by P. nodorum. The test pathogen showed 100% homology with other reported P. nodorum isolates by rDNA (ribosomal DNA) analysis. In addition, the amplification of rDNA region of 36 P. nodorum isolates representing various agro-ecological areas of HP and one infected wheat leaf sample generated an amplicon of ~ 449-bp with JB433 (5′-ACACTCAGTAGTTTACTACT-3′) and JB434 (5′-TGTGCTGCGCTTCAATA-3′) P. nodorum specific primer pair whereas no amplification was observed with the genomic DNA of Septoria titici, Stemphylium vesicarium and healthy wheat leaf sample. This study on integration of morpho-cultural and microscopic methods along with PCR based technique could form basis for routine diagnosis of the SLGB in wheat samples during early growth stages of crop in the seed production fields.


Glume blotch Wheat Prevalence Stagonospora nodorum PCR 



Stagonospora leaf and glume blotch


Minimal agar medium


Cetyl trimethylammonium bromide


Deoxyribonucleic acid


Polymerase chain reaction


Enzyme linked immunosorbent assay


National Centre for Biotechnology Information


Molecular Evolutionary Genetics Analysis


Internal transcribed spacer


Ribosomal DNA



The corresponding author is grateful to Department of Science and Technology, Govt of India for financial assistance.

Compliance with ethical standards

Conflict of Interest

All the authors declare that there has no conflict of interest.

Supplementary material

13562_2018_481_MOESM1_ESM.doc (58 kb)
Supplementary material 1 (DOC 58 kb)


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Copyright information

© Society for Plant Biochemistry and Biotechnology 2018

Authors and Affiliations

  1. 1.Department of Plant PathologyCSK HP Krishi VishvavidyalayaPalampurIndia

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