Estimation of nuclear genome size and characterization of Ty1-copia like LTR retrotransposon in Mesua ferrea L.
Mesua ferrea L. is a multipurpose versatile tree that is well known as a prospective feedstock biodiesel plant. The potentiality of M. ferrea as a sustainable source of feedstock for biodiesel industry is dependent on an extensive knowledge of the genome structure of the plant. However, to date, there has been no genomic research aimed at the exploitation of the biotechnological potential of this species. Flow cytometry with propidium iodide as the DNA stain was used to estimate the nuclear DNA content of Mesua and the 2C value is estimated to be 1.40 ± 0.02 pg. Somatic chromosome count from root-tip cells is found to be 2n = 30 corresponding to the diploid level (n = 15). Fold variation in genome size (1.14) is observed among the plants collected from different geographic locations in Assam and is attributed to reverse transcriptase-RNase H (RT-RH) domains of the Ty1-copia retrotransposons. Dot blot analysis revealed that Ty1-copia accounts for 2.5% of total haploid nuclear genome of Mesua and phylogenetic analyses showed that the RT-RH sequences are heterogeneous and resolved into 3 distinct groups. These results contribute to our understanding about genome organization of Mesua and will provide valuable information for its utilization in future.
KeywordsFlow cytometry Mesua ferrea Ty1-copia Nuclear DNA content
RD and RGS thank Ministry of Human Resources Development (MHRD), Government of India for student fellowship. Thanks also to Department of Biosciences and Bioengineering, IITG for instrumentation facility.
Conceived and designed the experiments: LR, RD, RGS, SM Performed the experiments: RD, RGS Analyzed the data: RD, RGS, SM Contributed reagents/materials/analysis tools: LR Manuscript preparation: LR, RD, RGS.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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