Isolation and expression analysis of eight MADS-box genes in peach (Prunus persica var. nectarina ‘Luxing’)
- 160 Downloads
The MADS-box transcription factor (TF) plays a crucial regulatory role in plant vegetative growth, flower and fruit development. Eight MADS-box genes (designated as PpMADS15, 16, 17, 26, 27, 36, 37, 38; GenBank accession nos. KU559581, KU559582, KU559583, KU559592, KU559593, KU559602, KU559603, KU559604, respectively) were isolated from ‘Luxing’ (Prunus persica var. nectarina ‘Luxing’) peach by homologous comparison and RT-PCR, which contained open reading frames (ORF) of 597, 750, 1062, 615, 699, 1 107, 678 and 564 bp, respectively. The results of phylogenetic analysis revealed that PpMADS15 belonged to the AG subgroup, PpMADS16 to the SEP subgroup, PpMADS17 to the MIKC* group, PpMADS26, 27, and 38 to the Mα group, and PpMADS36 and 37 to the Mγ group. The results of the prediction for subcellular localization showed that eight PpMADS proteins were located in the nucleus. The results of promoter analysis indicated that there were multiple putative cis-acting elements that were involved in responsiveness to the following variables: light, defense and stress, low-temperature, heat stress, wound, fungal elicitor, anaerobic induction, MeJA, gibberellin, ABA, auxin, and SA. RT-PCR results showed that PpMADS15 was expressed in leaves, stems, roots, sepals, ovaries, stamens, petals, during flower and fruit development. PpMADS16 was expressed in stems, sepals, ovaries, stamens, petals, during flower and fruit development. PpMADS17 was expressed in stems, sepals, ovaries, stamens, petals, during flower and fruit development (except for 30 d). All members in the Mα and Mγ subgroups were expressed in roots, stems, leaves, sepals, ovaries, stamens, petals and during flower development, but PpMADS27 was expressed only during fruit development. These results suggested that eight PpMADS genes played a crucial regulatory role in vegetative growth, flower and fruit development of peaches.
Keywords‘Luxing’ peach MADS-box Transcription factor Gene cloning Expression analysis Bioinformatics
Opening reading frame
Quantitative real-time PCR
This study was supported by National Natural Science Foundation of China (Grant No. 31501742), Shandong Agricultural Good Cultivar Project (Grant No. 2016LZGC034), and Key Research and Development Plan (Major Key Technologies) of Shandong Province (Grant No. 2016ZDJS10A01). I would like to thank Professor Thomas Alan Gavin, Cornell University, and Xu Yi, for help with editing the English in this paper.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Dong QL, Yu XM, Liu DD, Wang HR, An M, Yao YX, Wang CJ (2013) Cloning of NAD-malic enzymes and their expression analysis during tissues and fruit development of apple. Acta Hortic Sin 40(4):739–748Google Scholar
- Giménez E, Pineda B, Capel J, Antón MT, Atarés A, Pérez-Martín F, García-Sogo B, Angosto T, Moreno V, Lozano R (2010) Functional analysis of the Arlequin mutant corroborates the essential role of the Arlequin/TAGL1 gene during reproductive development of tomato. PLoS ONE 5(12):e14427CrossRefGoogle Scholar
- Gu YB, Ji ZR, Chi FM, Qiao Z, Xu CN, Zhang JX, Zhou ZS, Dong QL (2016) Genome-wide identification and expression analysis of the WRKY gene family in peach. Hereditas (Beijing) 38(3):254–270Google Scholar
- Liu JH, Xu BY, Zhang J, Jin ZQ (2010) The interaction of MADS-box transcription factors and manipulating fruit development and ripening. Hereditas (Beijing) 32(9):893–902Google Scholar
- Ma J, Sun W, Wan J, Mu S, Li M (2014) Cloning and expression analysis of a late embryogenesis abundant protein gene CpLEA from Chimonanthus praecox. Acta Hortic Sin 41(8):1663–1672Google Scholar
- Paenicová L, de-Folter S, Kieffer M, Horner DS, Favalli C, Busscher J, Cook HE, Ingram RM, Kater MM, Davies B, Angenent GC, Colombo L (2013) Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in Arabidopsis: new openings to the MADS world. Plant Cell 15(7):1538–1551CrossRefGoogle Scholar
- Verde I, Abbott AG, Scalabrin S, Jung S, Shu S, Marroni F, Zhebentyayeva T, Dettori MT, Grimwood J, Cattonaro F, Zuccolo A, Rossini L, Jenkins J, Vendramin E, Meisel LA, Decroocq V, Sosinski B, Prochnik S, Mitros T, Policriti A, Cipriani G, Dondini L, Ficklin S, Goodstein DM, Xuan P, del Fabbro C, Aramini V, Copetti D, Gonzalez S, Horner DS, Falchi R, Lucas S, Mica E, Maldonado J, Lazzari B, Bielenberg D, Pirona R, Miculan M, Barakat A, Testolin R, Stella A, Tartarini S, Tonutti P, Arús P, Orellana A, Wells C, Main D, Vizzotto G, Silva HS, Alamini F, Schmutz J, Morgante M, Rokhsar MD (2013) The high-quality draft genome of peach (Prunus persica) identifies unique patterns of genetic diversity, domestication and genome evolution. Nat Genet 45(5):487–493CrossRefGoogle Scholar
- Wang CZ, Yu XM, Dong QL, Zhang AN, Liu W, Dong F, Wang SZ, Wang CJ (2015) Bioinformatic and expression analysis on the known MADS-box transcription factors at different development stages of flower in peach. J Nucl Agric Sci 29(5):849–858Google Scholar