Journal of Plant Biochemistry and Biotechnology

, Volume 27, Issue 4, pp 415–424 | Cite as

Genetic diversity changes in Indian lentils over the times

  • Jitendra KumarEmail author
  • Sunanda Gupta
  • Sonali Dubey
  • Priyanka Gupta
  • Debjyoti Sen Gupta
  • N P Singh
Original Article


The genetic diversity of 96 genotypes of lentil comprising 34 cultivars, 46 advanced breeding lines, and 16 germplasm lines were studied using 260 SSR markers. These markers generated a total of 749 alleles. The alleles/locus ranged from 2 to 16 with an average value of 2.87. Polymorphic information content varied from 0.02 to 0.91 with a mean of 0.30. Major allelic frequency ranged from 0.14 to 0.99 with a mean of 0.77. Studied genotypes were clustered into two groups according to their breeding history. Advanced breeding lines derived from exotic lines were clustered in one group, while another group accommodated most of the cultivars and advanced breeding lines with common cultivars in parentage. The germplasm lines were sub-clustered within first group. Cumulatively, first three principal components contributed 21.2% to the total variability. Advanced breeding lines showed higher number of alleles/locus and gene diversity (He) than other sets of genetic materials. In present study, no significant differences were observed between cultivars developed in different decadal groups for both NA and He. Moreover, genetic diversity changes between small and large seeded lentil cultivars were also found non-significant in this study. These findings showed that the use of alien genes can help to diversify active gene pool for developing improved new cultivars in lentil.


Genetic diversity Allelic diversity Gene diversity AMOVA SSR markers UPGMA Principal coordinate analysis 



Authors thank to Indian Council of Agricultural Research, New Delhi for research support. This work is partially funded by Department of Agriculture Corporation and Farmers’ Welfare (DAC & FW), Government of India, New Delhi and Department of Biotechnology (Grant No.: BT/PR10921/AG11/106/943/2014), Govt. of India, New Delhi.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

13562_2018_450_MOESM1_ESM.docx (37 kb)
Supplementary material 1 (DOCX 37 kb)


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Copyright information

© Society for Plant Biochemistry and Biotechnology 2018

Authors and Affiliations

  • Jitendra Kumar
    • 1
    Email author
  • Sunanda Gupta
    • 1
  • Sonali Dubey
    • 1
  • Priyanka Gupta
    • 1
  • Debjyoti Sen Gupta
    • 1
  • N P Singh
    • 1
  1. 1.Division of Crop ImprovementICAR-Indian Institute of Pulses ResearchKanpurIndia

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