A rice bZIP transcription factor, OsbZIP16, regulates abiotic stress tolerance when over-expressed in Arabidopsis
- 282 Downloads
Plants exposed to adverse environmental conditions are invariably compromised in their growth and development. The bZIP class of transcription factors (TF) form a large group among stress signalling components that regulate plant responses towards stress. We identified bZIP TF encoding genes that are expressed differentially in indica rice under stress and here we functionally characterize one such gene, OsbZIP16. Although, OsbZIP16 forms a clade with its orthologous monocot protein sequences, we find in our study that it can impart tolerance to abiotic stress in Arabidopsis. OsbZIP16 is expressed strongly upon dehydration, salt and ABA treatment in Oryza sativa cv. IR64 seedlings. It localizes in the cell nucleus and the gene product is capable of transcriptional activation, thus providing evidence for its capability as a functional TF. Upon overexpression in Arabidopsis, OsbZIP16ox plants show wild type morphology, however, these plants showed tolerance when subjected to drought stress at vegetative stage and set healthy seeds on recovery. The OsbZIP16ox seedlings showed reduced sensitivity to mannitol, ABA and sodium chloride during germination and also reduced ROS accumulation upon H2O2 exposure. Thus, OsbZIP16 regulates abiotic stress responses and is also a good candidate gene that can be utilised to impart tolerance in plants under water deficit conditions.
KeywordsbZIP ROS Drought Arabidopsis Rice
Oryza sativa basic leucine zipper
Reactive oxygen species
This work was financially supported by a grant (BT/PR12394/AGIII/103/891/2014) from the Department of Biotechnology, Government of India. ASP, ES and NB are grateful to CSIR and NJ to UGC for the award of research fellowships. The infrastructure support provided by the Department of Science and Technology, Government of India, and the University Grants Commission, New Delhi, is also acknowledged.
Compliance with ethical standards
Conflict of interest
The authors declare they have no competing financial conflicts of interest.
- Huang XS, Liu JH, Chen XJ (2010) Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes. BMC Plant Biol. https://doi.org/10.1186/1471-2229-10-230 CrossRefPubMedPubMedCentralGoogle Scholar
- Nakagawa H, Ohmiya K, Hattori T (1996) A rice bZIP protein, designated OSBZ8, is rapidly induced by abscisic acid. Plant J 9:217–227. https://doi.org/10.1046/J.1365-313x.1996.09020217.X CrossRefPubMedGoogle Scholar
- Pandey AS (2015) Functional characterization of some rice bZIP transcription factor genes involved in stress tolerance and seed development (PhD thesis). University of DelhiGoogle Scholar
- Todaka D, Shinozaki K, Yamaguchi-Shinozaki K (2015) Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants. Front Plant Sci 6:84. https://doi.org/10.3389/fpls.2015.00084 CrossRefPubMedPubMedCentralGoogle Scholar
- Xiang Y, Tang N, Du H et al (2008) Characterization of OsbZIP23 as a key player of the basic leucine zipper transcription factor family for conferring abscisic acid sensitivity and salinity and drought tolerance in rice. Plant Physiol 148:1938–1952. https://doi.org/10.1104/pp.108.128199 CrossRefPubMedPubMedCentralGoogle Scholar