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Journal of Plant Biochemistry and Biotechnology

, Volume 27, Issue 4, pp 393–400 | Cite as

A rice bZIP transcription factor, OsbZIP16, regulates abiotic stress tolerance when over-expressed in Arabidopsis

  • Agni Shekhar Pandey
  • Eshan Sharma
  • Nitin Jain
  • Brinderjit Singh
  • Naini Burman
  • Jitendra P. Khurana
Original Article
  • 201 Downloads

Abstract

Plants exposed to adverse environmental conditions are invariably compromised in their growth and development. The bZIP class of transcription factors (TF) form a large group among stress signalling components that regulate plant responses towards stress. We identified bZIP TF encoding genes that are expressed differentially in indica rice under stress and here we functionally characterize one such gene, OsbZIP16. Although, OsbZIP16 forms a clade with its orthologous monocot protein sequences, we find in our study that it can impart tolerance to abiotic stress in Arabidopsis. OsbZIP16 is expressed strongly upon dehydration, salt and ABA treatment in Oryza sativa cv. IR64 seedlings. It localizes in the cell nucleus and the gene product is capable of transcriptional activation, thus providing evidence for its capability as a functional TF. Upon overexpression in Arabidopsis, OsbZIP16ox plants show wild type morphology, however, these plants showed tolerance when subjected to drought stress at vegetative stage and set healthy seeds on recovery. The OsbZIP16ox seedlings showed reduced sensitivity to mannitol, ABA and sodium chloride during germination and also reduced ROS accumulation upon H2O2 exposure. Thus, OsbZIP16 regulates abiotic stress responses and is also a good candidate gene that can be utilised to impart tolerance in plants under water deficit conditions.

Keywords

bZIP ROS Drought Arabidopsis Rice 

Abbreviations

OsbZIP

Oryza sativa basic leucine zipper

ABA

Abscisic acid

ROS

Reactive oxygen species

TFs

Transcription factors

Notes

Acknowledgements

This work was financially supported by a grant (BT/PR12394/AGIII/103/891/2014) from the Department of Biotechnology, Government of India. ASP, ES and NB are grateful to CSIR and NJ to UGC for the award of research fellowships. The infrastructure support provided by the Department of Science and Technology, Government of India, and the University Grants Commission, New Delhi, is also acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare they have no competing financial conflicts of interest.

Supplementary material

13562_2018_448_MOESM1_ESM.pdf (759 kb)
Supplementary material 1 (PDF 759 kb)

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Copyright information

© Society for Plant Biochemistry and Biotechnology 2018

Authors and Affiliations

  • Agni Shekhar Pandey
    • 1
    • 2
  • Eshan Sharma
    • 1
    • 2
  • Nitin Jain
    • 1
    • 2
  • Brinderjit Singh
    • 1
    • 2
  • Naini Burman
    • 1
    • 2
  • Jitendra P. Khurana
    • 1
    • 2
  1. 1.Interdisciplinary Centre for Plant GenomicsUniversity of Delhi South CampusNew DelhiIndia
  2. 2.Department of Plant Molecular BiologyUniversity of Delhi South CampusNew DelhiIndia

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