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Increased Blood Lead Level Alters the Heme Biosynthesis and Haematological Parameters of Spray Painters from Western Maharashtra, India

  • Mandakini S. Kshirsagar
  • Arun J. PatilEmail author
  • Jyotsna A. Patil
Original Article

Abstract

Objective

To assess the present status of blood lead levels and their effect on heme biosynthesis and hematological parameters of spray painters.

Methods

Forty two spray painters having the age range of 25–50 years were recruited for the study from Western Maharashtra, India and compared with age matched 50 healthy male subjects. Blood lead (PbB), erythrocytes d-ALDH, Urinary d-ALA, PBG and hematological parameters were measured.

Results

PbB levels (p<0.001, 458%) of spray painters were significantly increased and Non-activated δ-ALAD (p<0.001, -20.21%), activated δ-ALAD (p<0.001, -28.25%) were significantly decreased and ratio of activated to non-activated δ-ALAD (p<0.001, 16%) was significantly increased in spray painters as compared to control subjects. Urinary δ-ALA (p<0.001, 160%) and PBG (p<0.001, 62.75%) levels were significantly increased in spray painters as compared to the control group. Hematological parameters of spray painters i.e. Hb (p<0.001, -21.58%) PCV (p<0.05, -4.53%) MCV (p<0.01, -5.09%) MCH (p<0.01, -6.04), MCHC (p<0.01, - 2.31) and RBC (p<0.001, - 8.06 %) were significantly decreased while total WBC count (p<0.001, 34.63%) was significantly increased as compared to the controls.

Conclusion

Increased PbB level in spray painters may be due to more absorption of lead thereby inhibiting heme biosynthesis enzymes and resulting in more excretion of urinary δ-ALA, PBG and alteration of hematological parameters. Therefore it is essential to monitor these spray painters and also it is necessary to create awareness for using adequate protective measures. Also necessary training and regular screening is required to minimize adverse impact of lead exposure to them.

Keywords

Spray painters Blood lead Heme biosynthesis Hematological parameters 

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Notes

Acknowledgements

We express our deep gratitude to all the spray painters who consented to volunteer in the project. We also acknowledge the research facilities provided by the Krishna Institute of Medical Sciences “Deemed To Be University”, Karad. Authors are also grateful to authors/ editors/publishers of all those articles, journals and books from where the literature for this article has been reviewed and discussed. The funds and the all research facilities are provided by the Krishna Institute of Medical Sciences “Deemed To Be University”, Karad.

References

  1. 1.
    The Editors of Encyclopedia Britannica. Lead. Chemical element, https://www.britannica.com/science/lead-chemical-element.pdf (2018).Google Scholar
  2. 2.
    Carocci, A., Catalano, A., Lauria, G., Sinicropi, M. S. & Genchi, G. Lead toxicity, antioxidant defense and environment. Rev. Environ. Contam. Toxicol. 238, 45–67 (2016).Google Scholar
  3. 3.
    Johnson, S., Saikia, N. & Sahu, R. Lead in Paints, http://indiaenvironmentportal.org.in/files/lead_paints.pdf (2009).Google Scholar
  4. 4.
    Van Alphen, M., Lead in Paints and Water in India. In Lead Poisoning Prevention & Treatment: Implementing a National Program in Developing Countries A.M. George (ed.) Banglore, India: The George Foundation, 265–272 (1999).Google Scholar
  5. 5.
    Clark, C. S. et al. Lead in paints and soil in Karnataka and Gujarat, India. J. Occup. Environ. Hyg. 2, 38–44 (2005).CrossRefGoogle Scholar
  6. 6.
    Buchet, J. P., Roels, H., Bernard, A. & Lauwerys, R. Assessment of renal function of workers exposed to inorganic lead, calcium or mercury vapor. J. Occup. Med. 22, 741–750 (1980).Google Scholar
  7. 7.
    Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological profile for lead. Atlanta, GA: US Department of Health and Human Services. CDC, Agency for Toxic Substances and Disease Registry. 2007.Google Scholar
  8. 8.
    Vitayavirasuk, B., Junhom, S. & Tantisaeranee, P. Exposure to lead, cadmium and chromium among spray painters in automobile body repair shops. J. Occup. Health 47, 518–522 (2005).CrossRefGoogle Scholar
  9. 9.
    Katharina Oginawati., Hermiranti Dwilestari. & Niko Junianto. Hematology Analysis of Lead Exposure on Painting Workers (Case Study: Informal Automobile Painting Industries in Karasak, Bandung). KnE Life Sciences. International Conference of Occupational Health and Safety, 674–686. https://www.knepublishing.com/index.php/Kne-Life/article/view/2597/5565 (2017).Google Scholar
  10. 10.
    Patil, A. J. et al. Occupational lead exposure in battery manufacturing workers, silver jewellery workers and spray painters in Western Maharashtra (India): effect on liver and kidney functions. J. Basic Clin. Physiol. Pharmacol. 18, 87–100 (2007).CrossRefGoogle Scholar
  11. 11.
    Stauber, J. L., Florence, T. M., Gulson, B. L. & Dale, L. S. Percutaneous absorption of inorganic lead compounds. Sci. Total Environ. 145, 55–70 (1994).CrossRefGoogle Scholar
  12. 12.
    Al-Modhefer, A. J. A., Bradbury, M. W. B. & Simmons, T. J. B. Observations on the chemical nature of lead in human blood serum. Clin. Sci. 81, 823–829 (1991).CrossRefGoogle Scholar
  13. 13.
    Agency for Toxic Substances and Disease Registry (ATSDR). Case studies in environmental medicine. Lead Toxicity. Course: WB2832; 9-161, https://www.atsdr.cdc.gov/csem/lead/docs/CSEM-Lead_toxicity_508.pdf (2017).Google Scholar
  14. 14.
    Agency for Toxic Substances and Disease Registry (ATSDR). Toxicological Profile for Cadmium, https://www.atsdr.cdc.gov/toxprofiles/tp5.pdf (2012).Google Scholar
  15. 15.
    IPCS, INCHEM, Environmental Health Criteria 165, Inorganic lead, World Health Organization 165, http://www.inchem.org/documents/ehc/ehc/ehc165.htm (1995).Google Scholar
  16. 16.
    Patil, A. J. et al. Biochemical aspects of lead exposure in silver jewellery workers of Western Maharashtra (India). J. Basic Clin. Physiol. Pharmacol. 17, 213–229 (2006).CrossRefGoogle Scholar
  17. 17.
    Dresner, D. L. Modulation of bone marrow heme and protein synthesis by trace elements. Environ. Res. 28, 55–66 (1982).CrossRefGoogle Scholar
  18. 18.
    Raghavan, S. R. V., Dwight Culver, B. & Harvey, C. Gonick. Erythrocyte lead-binding protein after occupational exposure. II. Influence on lead inhibition of membrane Na+, K+-adenosinetriphosphatase. J. Toxicol. Environ. Health 7, 561–568 (1981).CrossRefGoogle Scholar
  19. 19.
    EPA. United State, Environmental Protection Agency, Air quality criteria for lead, https://cfpub.epa.gov/ncea/risk/recordisplay.cfm?deid=32647 (1986).Google Scholar
  20. 20.
    Paglia, D. E., Valentine, W. N. & Dahigren, J. G. Effects of low-level lead exposure on pyrimidine 5′nucleotidase and other erythrocyte enzymes: Possible role of pyrim-idine 5′-nucleotidase in the pathogenesis of lead-induced anemia. J. Clin. Invest. 56, 1164–1169 (1975).CrossRefGoogle Scholar
  21. 21.
    Paglia, D. E., Valentine, W. N. & Fink, K. Lead poisoning: Further observations on erythrocyte pyrimidine-nucleotidase deficiency and intracellular accumulation of pyrimidine nucleotides. J. Clin. Invest. 60, 1362–1366 (1977).CrossRefGoogle Scholar
  22. 22.
    Shannon, M. W. & Townsend, M. K. Adverse effects of reduced-dose d-penicillamine in children with mild to moderate lead poisoning. Ann. Pharmacother. 34, 15–18 (2000).CrossRefGoogle Scholar
  23. 23.
    Declaration of Helsinki, 1964. Amended by World Medical Assembly. Venice, Italy. British Medical Journal l313, 1448–1449 (1996).Google Scholar
  24. 24.
    LeadCare® II Blood Lead Analyzer User’s Guide, {rs http://www.cantonhealth.org/pdf/400-001-08-06A_Blood%20LeadII%20Users%20Guide.pdf} (2015).Google Scholar
  25. 25.
    Chisolm, J. J., Jr, Thomas, D. J. & Hamill, T. G. Eryth-rocyte porphobilinogen synthase activity as an indicator of lead exposure in children. Clin. Chem. 31, 601–605 (1985).Google Scholar
  26. 26.
    Osamu, W., Kohei, T., Gumpei, U., Yuzo, Y. & Kiku, N. A simple method for the quantitative analysis of urinary delta-aminol evulinic acid to evaluate lead absorption. Br. J. Ind. Med. 26, 240–243 (1969).Google Scholar
  27. 27.
    Mauzerall, D. & Granick, S. The occurrence and determination of d-aminolevulinic acid and porphobilinogen in urine. J. Biol. Chem. 219, 435–446 (1956).Google Scholar
  28. 28.
    Rimington, C. Quantitative Determination of Porpho-bilinogen and Porphyrins in Urine and Faeces. Assoc. Clin. Pathol., London (1961).Google Scholar
  29. 29.
    Trester, C. Modern Technology for Cellular Differentiation: Principles of Measurement in Sysmex Haematology Analyzers: Technology Update in Laboratory Haematology. Sysmex Scientific Semina, India (1999).Google Scholar

Copyright information

© The Korean Society of Environmental Risk Assessment and Health Science and Springer 2019

Authors and Affiliations

  • Mandakini S. Kshirsagar
    • 1
  • Arun J. Patil
    • 1
    Email author
  • Jyotsna A. Patil
    • 1
  1. 1.Department of BiochemistryKrishna Institute of Medical SciencesKaradIndia

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