Advertisement

Toxicology and Environmental Health Sciences

, Volume 10, Issue 5, pp 261–267 | Cite as

The Effect of Antioxidant (Gallic acid) on the Testes of Lead Acetate Induced Wistar Rat

  • Taiwo Hassan Bello
  • Oladayo Amed IdrisEmail author
Original article
  • 3 Downloads

Abstract

Objective

Lead is considered one of the major causes of infertility among occupational workers in manufacturing companies such as electric storage batteries, glass and ceramic wares. This study was carried out to confirm the effect of Gallic acid as an antioxidant on Lead acetate treated male albino rats.

Methods

Twenty albino rats weighing between 140 g and 200 g were divided equally into four groups. Group A served as the control, group B was treated with 60 mg/kg body weight of Lead acetate, group C received 50 mg/kg body weight of Gallic acid and group D received both Lead acetate and Gallic acid for 14 days.

Results

The result showed slight increase in the body weights and testes of group B and D compared to the control group and there was a slight decrease in group C. Rats in group B showed a significant increase in MDA (3224.63±89.38 unit/mg protein) but a slight increase was observed in the level of MDA (1537.70± 53.92 unit/mg protein) in group D. The level of Lactate Dehydrogenase (LDH) in group B was 338.75±18.87 nmol min mL, which was the highest among the groups and significant.

Conclusion

Lead acetate is toxic to the gonad, it causes degeneration and reduction in spermatogenic activity in seminiferous tubules. However, the effect of Lead on the male reproductive organ can be reverse significantly by an antioxidant (gallic acid) due to its ability to scavenge for oxidative stress caused by Lead.

Keywords

Lead acetate Spermatogenesis Toxicity Gallic acid Testes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Vigeh, M., Smith, D. R. & Hsu, P.–C. How does lead induce male infertility? Iran. J. Reprod. Med. 9, 1–8 (2011).Google Scholar
  2. 2.
    Sallmén, M. International journal of occupational medicine and environmental health. Int. J. Occup. Med. Environ. Healt. 14, 219–222 (2001).Google Scholar
  3. 3.
    Adebayo Kehinde, B. & Ayoola Isaac, J. Effects of Heat and Lead on the Reproductive System of Male Wistar Rat. Anat. Sci. J. 11, 99–106 (2014).Google Scholar
  4. 4.
    Olayemi, F. O. A review on some causes of male infertility. Afr. J. Biotechnol. 9, 2834–2842 (2002).Google Scholar
  5. 5.
    Assi, M. A., Hezmee, M. N. M., Haron, A. W., Sabri, M. Y. M. & Rajion, M. A. The detrimental effects of lead on human and animal health. Vet. Worl. 9, 660–671 (2016).CrossRefGoogle Scholar
  6. 6.
    Queiroz, E. K. R. de & Waissmann, W. Occupational exposure and effects on the male reproductive system. Cad. Saude Public. 22, 485–493 (2006).CrossRefGoogle Scholar
  7. 7.
    AIT Hamadouche, N. Reproductive toxicity of lead acetate in adult male rats. Am. J. Sci. Res. 3, 38–50 (2009).Google Scholar
  8. 8.
    Sanders, T., Liu, Y., Buchner, V. & Tchounwou, P. B. Neurotoxic Effects and Biomarkers of Lead Exposure: A Review. Rev. Environ. Healt. 24, 15–45 (2009).CrossRefGoogle Scholar
  9. 9.
    Needleman, H. L. & Landrigan, P. J. What level of lead in blood is toxic for a child? Am. J. Public Healt. 94, 8 (2004).CrossRefGoogle Scholar
  10. 10.
    Bonde, J. P. et al. Sperm count and chromatin structure in men exposed to inorganic lead: lowest adverse effect levels. Occup. Environ. Med. 59, 234–242 (2002).CrossRefGoogle Scholar
  11. 11.
    Idris, O. A., Wintola, O. A. & Afolayan, A. J. Phytochemical and antioxidant activities of Rumex crispus L. in treatment of gastrointestinal helminths in Eastern Cape Province, South Africa. Asian Pac. J. Trop. Biomed. 7, 1071–1078 (2017).CrossRefGoogle Scholar
  12. 12.
    Fuchs–Tarlovsky, V. Role of antioxidants in cancer therapy. Nutritio. 29, 15–21 (2013).Google Scholar
  13. 13.
    Li, Z.–J. et al. Antifungal Activity of Gallic Acid In Vitro and In Vivo. Phyther. Res. 31, 1039–1045 (2017).CrossRefGoogle Scholar
  14. 14.
    Jin, L. et al. Gallic Acid Reduces Blood Pressure and Attenuates Oxidative Stress and Cardiac Hypertrophy in Spontaneously Hypertensive Rats. Sci. Rep. 7, doi: 10. 1038/s41598–017–15925–1 (2017).Google Scholar
  15. 15.
    Hassan, A. I. & Alam, S. S. Evaluation of mesenchymal stem cells in treatment of infertility in male rats. Stem Cell Res. Ther. 5, doi: 10.1186/scrt521 (2014).Google Scholar
  16. 16.
    Martinez–Saez, N. et al. A novel antioxidant beverage for body weight control based on coffee silverskin. Food Chem. 150, 227–234 (2014).CrossRefGoogle Scholar
  17. 17.
    Sujatha, K., Srilatha, C. H., Rao, T. S. C. & Amaravathi, P. Ultrastructural and histopathological studies in lead acetate induced neurotoxicity in wistar albino rats and its amelioration with Ocimum sanctum (os) a leaf extract. Int. J. Pharma Bio Sci. 2, 295–304 (2011).Google Scholar
  18. 18.
    Canbek, M. et al. The effect of gallic acid on kidney and liver after experimental renal ischemia/reperfusion injury in the rats. African J. Pharm. Pharmacol. 5, 1027–1033 (2011).Google Scholar
  19. 19.
    Kobyliak, N. M. et al. Antioxidative effects of cerium dioxide nanoparticles ameliorate age–related male infertility: optimistic results in rats and the review of clinical clues for integrative concept of men health and fertility. EPMA J. 6, doi: 10.1186/s13167–015–0034–2 (2015).Google Scholar
  20. 20.
    Varshney, R. & Kale, R. K. Effects of Calmodulin Antagonists on Radiation–induced Lipid Peroxidation in Microsomes. Int. J. Radiat. Biol. 58, 733–743 (1990).CrossRefGoogle Scholar
  21. 21.
    Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951).Google Scholar
  22. 22.
    Kumar, V. & Gill, K. D. in Basic Concepts in Clinical Biochemistry: A Practical Guide (Springer, Singapore, 2018).CrossRefGoogle Scholar

Copyright information

© Korean Society of Environmental Risk Assessment and Health Science and Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of Basic Medical Science, Obafemi Awolowo College of Health SciencesOlabisi Onabanjo UniversityOgun StateNigeria
  2. 2.Medicinal Plants and Economic Development (MPED) Research Centre, Department of BotanyUniversity of Fort HareAliceSouth Africa

Personalised recommendations