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Association of apolipoprotein A-V with mRNA expression of IL-6 and NF-κB genes in type 2 diabetes with hypertriglyceridemia: a possible link with inflammation

  • Devesh Sharma
  • Seema GargEmail author
  • Mohit Mehndiratta
  • S. V. Madhu
  • Dinesh Puri
Original Article
  • 5 Downloads

Abstract

Background

Hypertriglyceridemia and inflammation are implicated in cardiovascular complications in type 2 diabetes mellitus (T2DM). While some studies have evaluated apolipoprotein A-V protein (Apo A-V) in diabetes and its effect on triglycerides (TG) levels, only a few have explored its relation with inflammatory markers.

Objectives

To evaluate the expression of mRNA genes involved in inflammatory response (IL-6 and NF-κB) and to study their association with Apo A-V in patients of T2DM, with and without hypertriglyceridemia.

Methods

Two groups of T2DM patients, comprising of 40 participants each, were constituted according to NCEP ATP III criteria for CVD risk: group 1/controls (TG ≤ 1.65 mmol/l) and group 2/cases (TG ≥ 2.2 mmol/l). Serum levels of Apo A-V, free fatty acids (FFA) and IL-6 were estimated. Fold change in mRNA expression of IL-6 and NF-κB (p65) gene in blood was calculated by ΔΔCT method.

Observations

The mRNA expression of IL-6 and p65 was higher in cases. Significant inverse association of Apo A-V levels was observed with expression of p65 gene (p = 0.000) and IL-6 gene (p = 0.003) in all subjects. FFA levels also correlated inversely with the expression of p65 (p = 0.001) and IL-6 (p = 0.005) mRNA. The association of FFA levels and ApoA-V with mRNA expression of IL-6 and p65 genes was independent of each other.

Conclusion

This study highlights that inflammatory pathways are unregulated in hypertriglyceridemia in T2DM. Also, apart from its association with TG levels, Apo A-V may have an anti-inflammatory role as evident from its inverse association with the expression of IL-6 and NF-κB expression. Thus, Apo A-V may provide an important link between TGs, inflammation, and vascular complications in T2DM.

Keywords

Apolipoprotein av., human Hypertriglyceridemia Type 2 diabetes mellitus Free fatty acids Nf kappa b p65 il6 

Notes

Acknowledgements

The authors convey their gratitude to the patients who participated in the study and to the technical staff of department of the Biochemistry and hospital lab services.

Glossary

Apo A-V Apolipoprotein A-V protein

Apo A5 Apolipoprotein A5 gene

TG Triglycerides

T2DM Type 2 diabetes mellitus

IL-6 Interleukin 6

NF-κB Nuclear factor-kappa B

P65 Nuclear factor-kappa B gene

FFA Free fatty acids

Funding information

Project was funded by the intramural grant obtained from parent institution, University College of Medical Sciences.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Statement of informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Research Society for Study of Diabetes in India 2019

Authors and Affiliations

  • Devesh Sharma
    • 1
  • Seema Garg
    • 2
    Email author
  • Mohit Mehndiratta
    • 1
  • S. V. Madhu
    • 3
  • Dinesh Puri
    • 1
  1. 1.Department of BiochemistryUniversity College of Medical Sciences, University of DelhiDelhiIndia
  2. 2.Department of BiochemistryAll India Institute of Medical SciencesNagpurIndia
  3. 3.Department of MedicineUniversity College of Medical Sciences, University of DelhiDelhiIndia

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