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Microwave-assisted pretreatment using alkali metal salt in combination with orthophosphoric acid for generation of enhanced sugar and bioethanol

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Abstract

The microwave-assisted alkali salt (NaCl, MgCl2, and KCl) in combination with H3PO4 pretreatment of rice straw followed by enzymatic hydrolysis with in-house and commercial enzyme cocktail (cellulase and xylanase) was investigated. The effect of pretreatment on the structure and composition of RS was investigated using XRD and FTIR analysis, and the result suggested strategic structural changes in the RS structure. The maximum CrI of 51.3% was obtained with KCl–H3PO4 pretreatment system which is in line with maximum saccharification per biomass of 79% using commercial xylanase and cellulase enzyme cocktail (ComCK–XylCell). Comparable saccharification per biomass of 74% was obtained with in-house xylanase and cellulase enzyme cocktail (CrCK–XylCell). The simultaneous saccharification and fermentation by KCl–H3PO4 pretreated system of RS resulted the maximum sugar yield of 30.6 and 26.9% and ethanol yield of 12.2 and 11.9 g/L using ComCK–XylCell and CrCK–XylCell cocktail, respectively, along with Saccharomyces cerevisiae MTCC 173. The hydrolysis of pretreated RS pulp using in-house enzyme cocktail resulted in high sugar and ethanol yield suggesting that the studied alkali metal in combination with weak acid such as H3PO4 can serve as a cost-effective and environment friendly pretreatment method for high production of sugar and ethanol.

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Acknowledgments

The authors acknowledge the support for instrumentation facility FTIR from Sophisticated Analytical Instrument Facility (SAIF), Indian Institute of Technology, Bombay, XRD from Department of Physics, Central University of Rajasthan (CURAJ), HPLC from School of Chemical Sciences, Central University of Rajasthan (CURAJ). Authors are also thankful to DST-FIST (SR/FST/LSI-676/2016) for necessary infrastructure facility generated at Department of Microbiology, CURAJ. NB acknowledges University Grants Commission for providing Rajiv Gandhi National Fellowship for doctoral studies. BK acknowledges Jawaharlal Nehru Memorial Fund, New Delhi, CSIR-SRF for providing funding for Doctoral Studies.

Funding

This work was financially supported by the Department of Biotechnology, Government of India through Project BT/304/NE/TBP/2012 and BT/PR7333/PBD/26/373/2012.

Author information

Pradeep Verma (PV) has played a vital role in conceptualization of research idea. Nisha Bhardwaj (NB) has conducted the laboratory work and prepared the rough draft of MS. Bikash Kumar (BK) has performed the formal analysis of the results and writing of the MS. The experimental, writing, and formal analysis was supervised by PV. In addition, PV’s role has key in acquisition of the financial supports for the project leading to this publication. All authors have given approval to the final version of the manuscript.

Correspondence to Pradeep Verma.

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Bhardwaj, N., Kumar, B. & Verma, P. Microwave-assisted pretreatment using alkali metal salt in combination with orthophosphoric acid for generation of enhanced sugar and bioethanol. Biomass Conv. Bioref. (2020). https://doi.org/10.1007/s13399-020-00640-1

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Keywords

  • Microwave-assisted pretreatment
  • Alkali metals
  • Orthophosphoric acid
  • Ethanol