Citric acid-based deep eutectic solvent for the anthocyanin recovery from Hibiscus sabdariffa through microwave-assisted extraction
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In the current study, Hibiscus sabdariffa has been extracted by microwave-assisted extraction (MAEX). Citric acid-based deep eutectic solvents (DES) have been specially designed, where a hydrogen bond donor (HBD) (glycerol and ethylene glycol) and a hydrogen bond acceptor (HBA) (citric acid) with a certain molar ratio (1/4) were used. After the best DES (citric acid/ethylene glycol) has been decided to extract the bioactive ingredients, operation conditions (power of microwave, volume of solvent water and content in the DES) of the MAEX for the relevant plant material have been optimized through Box-Behnken design (BBD) of response surface approach (RSA). The maximum yields of total phenolics (TP), total anthocyanins (TAA) and antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) have been found as 31.897 mg-GAE/g-DH, 2.961 mg-C3G/g-DH and 95.887% under the optimal conditions (35 mL DES including 50% water (v/v) at 550 W power of microwave). The differences between the experimental and estimated findings were lower than 2%. Three replicate test results obtained by in vitro experiments were also statistically analysed by using one-way analysis of variance (ANOVA) test.
KeywordsDeep eutectic solvent Anthocyanin Antioxidant activity Hibiscus sabdariffa Microwave-assisted extraction Box-Behnken
The authors thank the Research Fund of Istanbul University-Cerrahpaşa for the financial support for this research project (Project No: BYP-2019-34032).
Compliance with ethical standards
Conflict of interest
The authors declare that there is no conflict of interest.
- 6.Borrás-Linares I, Fernández-Arroyo S, Arráez-Roman D et al (2015) Characterization of phenolic compounds, anthocyanidin, antioxidant and antimicrobial activity of 25 varieties of Mexican Roselle (Hibiscus sabdariffa). Ind Crop Prod 69:385–394. https://doi.org/10.1016/j.indcrop.2015.02.053 CrossRefGoogle Scholar
- 12.Fernández-Arroyo S, Rodríguez-Medina IC, Beltrán-Debón R et al (2011) Quantification of the polyphenolic fraction and in vitro antioxidant and in vivo anti-hyperlipemic activities of Hibiscus sabdariffa aqueous extract. Food Res Int 44:1490–1495. https://doi.org/10.1016/j.foodres.2011.03.040 CrossRefGoogle Scholar
- 13.Almeida PS, Nascimento CCHC, Nascimento SF, Gomes ML, De Vasconcelos SDD, Azevedo LAC, Stephens PRS, Diré GF, Barreto AS (2018) Evaluation of the antioxidant, antimicrobial, cytotoxic and genotoxic activities of the aqueous extract of chalices of Hibiscus Sabdariffa Linn. Eur J Biomed Pharm Sci 5:31–44Google Scholar
- 26.Ateş F, Şahin S, İlbay Z, Kırbaşlar Şİ (2017) A green valorisation approach using microwaves and supercritical CO2 for high-added value ingredients from mandarin (Citrus deliciosa Tenore) leaf waste. Waste and Biomass Valorization 1–14. doi: https://doi.org/10.1007/s12649-017-0074-z CrossRefGoogle Scholar
- 27.Malik NSA, Bradford JM (2008) Recovery and stability of oleuropein and other phenolic compounds during extraction and processing of olive (Olea europaea L.) leaves. J Food Agric Environ 6:8–13Google Scholar
- 28.Lee J, Durst RW, Wrolstad RE (2005) Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. J AOAC Int 88:1269–1278Google Scholar
- 40.Elez Garofulić I, Dragović-Uzelac V, Režek Jambrak A, Jukić M (2013) The effect of microwave assisted extraction on the isolation of anthocyanins and phenolic acids from sour cherry Marasca (Prunus cerasus var. Marasca). J Food Eng 117:437–442. https://doi.org/10.1016/j.jfoodeng.2012.12.043 CrossRefGoogle Scholar
- 41.Zheng X, Liu B, Li L, Zhu X (2011) Microwave-assisted extraction and antioxidant activity of total phenolic compounds from pomegranate peel. J Med Plant Res 5:1004–1011Google Scholar